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The map label for this gene is insK [C]

Identifier: 157368623

GI number: 157368623

Start: 423337

End: 424161

Strand: Direct

Name: insK [C]

Synonym: Spro_0376

Alternate gene names: 157368623

Gene position: 423337-424161 (Clockwise)

Preceding gene: 157368622

Following gene: 157368624

Centisome position: 7.77

GC content: 54.91

Gene sequence:

>825_bases
ATGTGTGGTGCGACCGGTCTGTCGCAACGTCGTGCCTGCAGGCTGACAGGTTTGTCCCTGTCGACCTGCCGCTATGAAGC
GCAGCGTCCGACGTCGGATGCGCATTTATCAGGGCGCATCACTGAACTGGCGCTTGAACGCAGGCGTTTTGGTTACCGGC
GCATCTGGCAGTTACTGCGTCGGGAGGGCGTTCACGTCAATCACAAGCGGGTATACCGCATTTACCATCTTAACAGCCTG
AGCGTAAAACGCAGACGACGTCGTAAAGGGCTGGCGACCGAGCGGTTTCCCCTTCTGCGCCCAGATGCGCCGAACCTGAC
CTGGTCGATGGATTTCGTCATGGACGCACTGGCCACCGGTCGCAGGATCAAGTGTCTGACCTGCGTGGATGATTTCACGA
AAGAGTGCCTGACGATCACCGCCGCTTTCGGGATTTCAGGCGTGCAGGTCATGCGTATTCTGGACAGCATTGCGCTGTTT
CGCGGCTATCCGGCGACGATAAGAACCGATCAGGGGCCGGAATTTACCTGCCGCGCATTGGAGCAATGGGCCTTTGAGCA
TGGCGTGGAGCTGCGGCTTATCCAGCCGGGCAAGCCGACCCAGAACGGATTTATTGAGAGTTTTAACGGACGCTTTCGCG
ATGAATGCCTGAATGAGCACTGGTTCAGCGATATTGTTCACGCCAGGAAAATCATTAATAACTGGCGACAAGATTATAAC
GAGTGCTGTCCTCATTCATCGCTGAACTACCAGACTCCAGCTGAATTTGCAGCTGGCTGGCGAAACAGGAAATATGAAGA
AAAACCAACCGACATTACTAACTGA

Upstream 100 bases:

>100_bases
AGAAGCTGCTCGCCGAAGCCATGCTCGATAAGGAGGCGCTTCAGGTGGCTCTTGGGCGAAAGTACTGACGACAGACCAGA
AGCGGGAAGCCGTGGAGTTG

Downstream 100 bases:

>100_bases
AGGTTGTATCTAATCCTGGGGGCAGGTCAATGAACCCATTGAGAAAAATTCTCATAAAAACCAGCATAGTTTAGTTTTGC
CTATTGGAACAATTACTGAG

Product: Integrase catalytic subunit

Products: NA

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MCGATGLSQRRACRLTGLSLSTCRYEAQRPTSDAHLSGRITELALERRRFGYRRIWQLLRREGVHVNHKRVYRIYHLNSL
SVKRRRRRKGLATERFPLLRPDAPNLTWSMDFVMDALATGRRIKCLTCVDDFTKECLTITAAFGISGVQVMRILDSIALF
RGYPATIRTDQGPEFTCRALEQWAFEHGVELRLIQPGKPTQNGFIESFNGRFRDECLNEHWFSDIVHARKIINNWRQDYN
ECCPHSSLNYQTPAEFAAGWRNRKYEEKPTDITN

Sequences:

>Translated_274_residues
MCGATGLSQRRACRLTGLSLSTCRYEAQRPTSDAHLSGRITELALERRRFGYRRIWQLLRREGVHVNHKRVYRIYHLNSL
SVKRRRRRKGLATERFPLLRPDAPNLTWSMDFVMDALATGRRIKCLTCVDDFTKECLTITAAFGISGVQVMRILDSIALF
RGYPATIRTDQGPEFTCRALEQWAFEHGVELRLIQPGKPTQNGFIESFNGRFRDECLNEHWFSDIVHARKIINNWRQDYN
ECCPHSSLNYQTPAEFAAGWRNRKYEEKPTDITN
>Mature_274_residues
MCGATGLSQRRACRLTGLSLSTCRYEAQRPTSDAHLSGRITELALERRRFGYRRIWQLLRREGVHVNHKRVYRIYHLNSL
SVKRRRRRKGLATERFPLLRPDAPNLTWSMDFVMDALATGRRIKCLTCVDDFTKECLTITAAFGISGVQVMRILDSIALF
RGYPATIRTDQGPEFTCRALEQWAFEHGVELRLIQPGKPTQNGFIESFNGRFRDECLNEHWFSDIVHARKIINNWRQDYN
ECCPHSSLNYQTPAEFAAGWRNRKYEEKPTDITN

Specific function: Involved in the transposition of the insertion sequence is150 [C]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1789981, Length=253, Percent_Identity=28.4584980237154, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1790724, Length=242, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789424, Length=242, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789224, Length=242, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788305, Length=242, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787669, Length=242, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786558, Length=242, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786462, Length=168, Percent_Identity=29.7619047619048, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 31901; Mature: 31901

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGATGLSQRRACRLTGLSLSTCRYEAQRPTSDAHLSGRITELALERRRFGYRRIWQLLR
CCCCCCCCHHHHHHHCCCCHHHHHHHCCCCCCCCHHCCHHHHHHHHHHHCCHHHHHHHHH
REGVHVNHKRVYRIYHLNSLSVKRRRRRKGLATERFPLLRPDAPNLTWSMDFVMDALATG
HCCCCCCHHEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCC
RRIKCLTCVDDFTKECLTITAAFGISGVQVMRILDSIALFRGYPATIRTDQGPEFTCRAL
CCEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHH
EQWAFEHGVELRLIQPGKPTQNGFIESFNGRFRDECLNEHWFSDIVHARKIINNWRQDYN
HHHHHHCCCEEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ECCPHSSLNYQTPAEFAAGWRNRKYEEKPTDITN
HHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MCGATGLSQRRACRLTGLSLSTCRYEAQRPTSDAHLSGRITELALERRRFGYRRIWQLLR
CCCCCCCCHHHHHHHCCCCHHHHHHHCCCCCCCCHHCCHHHHHHHHHHHCCHHHHHHHHH
REGVHVNHKRVYRIYHLNSLSVKRRRRRKGLATERFPLLRPDAPNLTWSMDFVMDALATG
HCCCCCCHHEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCC
RRIKCLTCVDDFTKECLTITAAFGISGVQVMRILDSIALFRGYPATIRTDQGPEFTCRAL
CCEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHH
EQWAFEHGVELRLIQPGKPTQNGFIESFNGRFRDECLNEHWFSDIVHARKIINNWRQDYN
HHHHHHCCCEEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ECCPHSSLNYQTPAEFAAGWRNRKYEEKPTDITN
HHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1718819 [H]