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The map label for this gene is dnaB [H]

Identifier: 157368551

GI number: 157368551

Start: 356933

End: 358288

Strand: Direct

Name: dnaB [H]

Synonym: Spro_0302

Alternate gene names: 157368551

Gene position: 356933-358288 (Clockwise)

Preceding gene: 157368550

Following gene: 157368552

Centisome position: 6.55

GC content: 53.61

Gene sequence:

>1356_bases
ATGAACCAGACAACATCCAATGTCCCTTATTCTTCCGATGCTGAGCAGGCAGTTCTCGGCGGTTTGATGCTGGATAATGA
GCGGTGGGATGATGTTCTCCCATTGGTTGGAGAGCGTGATTTTCATCTGGCGGTACATCGCCGAATTTTCCAGGCCATGG
CTCGCCTGATGGCTGTTCAGCAGCCGATTGATCTGATCACGCTCCATGAATCACTGGAACAAAATGGTGAGCTCGAGCAA
GTGGGCGGTTTTGCCTATTTGGCTGAATTGTCTAAAAACACGCCGAGTGCAGCCAATATCGTCGCCTATGCGGAAATTGT
GGCCGAACGCAGTCGTCTGCGTAGCCTGTTGCAACTCGGTCGTGAATTAAGCGCGGATGCTGCCAATCCCCGCGCTGAGT
CTTCTGCCCTGACCGAACGGGCAGAACAGCAATTGTTCCAGCTCTCGGAGCAAACCCAGTCAGAGCAGAATGTCAGCCTG
ACGGTTGGGTTGAATGCTGTCGTTACCTATCTTGAGACTGCGAATGGCGGCAATGGTATCACCGGCACCCCGACCGGTTT
CTCACGGCTTGATGAGATGACGTGTGGTTTACAGCCTGGTGATTTGATTTTGATTGCCGCACGGCCGTCGATGGGTAAAA
CCGCCTTTGCGTTGAGCACCTACACTGGCGCGCTTCGTGGCACGGAGAAACCTGCATTCTTTTTCAGCCTGGAAATGCCG
CGCGAGCAGTTGCTTCAACGCCTTATCGCTGTGGATGGACGTGTTGAACTTTCGCGTCTACGCAGTGGCCAGTTGGAAGA
TGAAGATTGGGCCCGAATTTCCAACGCGATGGAGCGGTTGTTGCCATTGGAAAACCGCCTGATTATTGACGATGAGTCAT
TTTTGACACCCGCTCTGTTGCGTTCCAGGGCACGGCGCTACACCCGGTTGTACGGGAAGCCTGGTTTAATCATGGTCGAT
TACCTGCAGCTGATGCGCTGCCCAGGGCAGGAAAATCGCACTCAGGAAATTGCGGAAATCTCCCGCAGCCTGAAAGCGTT
GGCCAAAGAACTTGATTGTCCCGTCGTGGCTCTATCTCAGTTGAACCGTCAGTTGGAAAGCCGTGCTGATAAACGGCCCT
TTAATGGTGATCTGCGTGACTCCGGTGCACTGGAGCAGGATGCTGATGTGATCGCGTTCATCTACCGTGATGAGGTTTAT
TACGCTGATTCCGATGATGCCGGCACGGCAGAAATTATCATTGGTAAACAGCGGCAGGGGGCGACGGGAACCGTTCGCGT
CAAGTTTGAAGGGCAATTCACCGCATTCAGCGACCTCCACGACGGTCAGTATGACCAATACTCGGGGAGGGCGTAA

Upstream 100 bases:

>100_bases
CGGCAGCCGCTGCCACTATGCACATGTTGGCCTGTGAGTTGTTCCCTCAGTGGCAATCAAACTTTGATGCGCTACTGGCA
GGGAAATCGGAGGTGCAGCC

Downstream 100 bases:

>100_bases
ACATGGCGCGTCCTAAAACCAAGATCCTCGATCTGAACAGTGCTTTGCTCCAGCAGGGCAAGACTGCAGCAGGCACACAG
CCTTTAGCCCCCGTGGTGGC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNQTTSNVPYSSDAEQAVLGGLMLDNERWDDVLPLVGERDFHLAVHRRIFQAMARLMAVQQPIDLITLHESLEQNGELEQ
VGGFAYLAELSKNTPSAANIVAYAEIVAERSRLRSLLQLGRELSADAANPRAESSALTERAEQQLFQLSEQTQSEQNVSL
TVGLNAVVTYLETANGGNGITGTPTGFSRLDEMTCGLQPGDLILIAARPSMGKTAFALSTYTGALRGTEKPAFFFSLEMP
REQLLQRLIAVDGRVELSRLRSGQLEDEDWARISNAMERLLPLENRLIIDDESFLTPALLRSRARRYTRLYGKPGLIMVD
YLQLMRCPGQENRTQEIAEISRSLKALAKELDCPVVALSQLNRQLESRADKRPFNGDLRDSGALEQDADVIAFIYRDEVY
YADSDDAGTAEIIIGKQRQGATGTVRVKFEGQFTAFSDLHDGQYDQYSGRA

Sequences:

>Translated_451_residues
MNQTTSNVPYSSDAEQAVLGGLMLDNERWDDVLPLVGERDFHLAVHRRIFQAMARLMAVQQPIDLITLHESLEQNGELEQ
VGGFAYLAELSKNTPSAANIVAYAEIVAERSRLRSLLQLGRELSADAANPRAESSALTERAEQQLFQLSEQTQSEQNVSL
TVGLNAVVTYLETANGGNGITGTPTGFSRLDEMTCGLQPGDLILIAARPSMGKTAFALSTYTGALRGTEKPAFFFSLEMP
REQLLQRLIAVDGRVELSRLRSGQLEDEDWARISNAMERLLPLENRLIIDDESFLTPALLRSRARRYTRLYGKPGLIMVD
YLQLMRCPGQENRTQEIAEISRSLKALAKELDCPVVALSQLNRQLESRADKRPFNGDLRDSGALEQDADVIAFIYRDEVY
YADSDDAGTAEIIIGKQRQGATGTVRVKFEGQFTAFSDLHDGQYDQYSGRA
>Mature_451_residues
MNQTTSNVPYSSDAEQAVLGGLMLDNERWDDVLPLVGERDFHLAVHRRIFQAMARLMAVQQPIDLITLHESLEQNGELEQ
VGGFAYLAELSKNTPSAANIVAYAEIVAERSRLRSLLQLGRELSADAANPRAESSALTERAEQQLFQLSEQTQSEQNVSL
TVGLNAVVTYLETANGGNGITGTPTGFSRLDEMTCGLQPGDLILIAARPSMGKTAFALSTYTGALRGTEKPAFFFSLEMP
REQLLQRLIAVDGRVELSRLRSGQLEDEDWARISNAMERLLPLENRLIIDDESFLTPALLRSRARRYTRLYGKPGLIMVD
YLQLMRCPGQENRTQEIAEISRSLKALAKELDCPVVALSQLNRQLESRADKRPFNGDLRDSGALEQDADVIAFIYRDEVY
YADSDDAGTAEIIIGKQRQGATGTVRVKFEGQFTAFSDLHDGQYDQYSGRA

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=443, Percent_Identity=56.2076749435666, Blast_Score=468, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50060; Mature: 50060

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQTTSNVPYSSDAEQAVLGGLMLDNERWDDVLPLVGERDFHLAVHRRIFQAMARLMAVQ
CCCCCCCCCCCCCHHHHHHCCEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
QPIDLITLHESLEQNGELEQVGGFAYLAELSKNTPSAANIVAYAEIVAERSRLRSLLQLG
CCHHHHHHHHHHHHCCCHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RELSADAANPRAESSALTERAEQQLFQLSEQTQSEQNVSLTVGLNAVVTYLETANGGNGI
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHCCCCCCC
TGTPTGFSRLDEMTCGLQPGDLILIAARPSMGKTAFALSTYTGALRGTEKPAFFFSLEMP
CCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEHHHHHHHHCCCCCCEEEEEECCC
REQLLQRLIAVDGRVELSRLRSGQLEDEDWARISNAMERLLPLENRLIIDDESFLTPALL
HHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHH
RSRARRYTRLYGKPGLIMVDYLQLMRCPGQENRTQEIAEISRSLKALAKELDCPVVALSQ
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LNRQLESRADKRPFNGDLRDSGALEQDADVIAFIYRDEVYYADSDDAGTAEIIIGKQRQG
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECEEEEECCCCCCCEEEEEECCCCC
ATGTVRVKFEGQFTAFSDLHDGQYDQYSGRA
CCCEEEEEECCCEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MNQTTSNVPYSSDAEQAVLGGLMLDNERWDDVLPLVGERDFHLAVHRRIFQAMARLMAVQ
CCCCCCCCCCCCCHHHHHHCCEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
QPIDLITLHESLEQNGELEQVGGFAYLAELSKNTPSAANIVAYAEIVAERSRLRSLLQLG
CCHHHHHHHHHHHHCCCHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RELSADAANPRAESSALTERAEQQLFQLSEQTQSEQNVSLTVGLNAVVTYLETANGGNGI
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHCCCCCCC
TGTPTGFSRLDEMTCGLQPGDLILIAARPSMGKTAFALSTYTGALRGTEKPAFFFSLEMP
CCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEHHHHHHHHCCCCCCEEEEEECCC
REQLLQRLIAVDGRVELSRLRSGQLEDEDWARISNAMERLLPLENRLIIDDESFLTPALL
HHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHH
RSRARRYTRLYGKPGLIMVDYLQLMRCPGQENRTQEIAEISRSLKALAKELDCPVVALSQ
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LNRQLESRADKRPFNGDLRDSGALEQDADVIAFIYRDEVYYADSDDAGTAEIIIGKQRQG
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECEEEEECCCCCCCEEEEEECCCCC
ATGTVRVKFEGQFTAFSDLHDGQYDQYSGRA
CCCEEEEEECCCEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]