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The map label for this gene is ubiE

Identifier: 157368497

GI number: 157368497

Start: 278646

End: 279401

Strand: Direct

Name: ubiE

Synonym: Spro_0248

Alternate gene names: 157368497

Gene position: 278646-279401 (Clockwise)

Preceding gene: 157368496

Following gene: 157368498

Centisome position: 5.11

GC content: 56.61

Gene sequence:

>756_bases
ATGGCAGATCAACCGCAGGAAACCACCGACTTCGGTTTTCGCACCGTAGCTAGAGACGAGAAGCAGGCCATGGTGGCAGA
TGTTTTTCATTCGGTAGCTGCAAAGTATGACGTGATGAACGACCTGATGTCGTTTGGTATCCACCGTATCTGGAAGCGTT
TTACCATTGACTGCAGCGGCGTTCGCCGTGGGCAGCGGGTGTTGGATCTGGCCGGTGGTACCGGTGACCTGGCCGCCAAG
TTCTCCCGCATGGTCGGTGAGCAGGGGCAGGTGGTACTGGCGGACATTAACGATTCCATGCTGAAGATGGGGCGCGAGAA
GCTGCGCGACCGCGGCATTATCGGTAACGTCAACTATGTGCAGGCCAATGCCGAAGCGCTGCCGTTCCCGGATAACTATT
TTGACTGCATTACCATTTCCTTCGGCCTGCGCAACGTGACCGACAAAGACAAAGCGCTGCGTTCCATGTTCCGCGTGCTC
AAGCCGGGCGGCCGTTTGCTGGTGCTGGAGTTCTCCAAGCCGCTGCTGGCGCCGTTAAGTAAAGCCTATGACGCCTACTC
GTTCCACGTGCTGCCGAAAATCGGGGAGCTGGTGGTGAAGGATCCGGAAAGTTACCGTTATCTGGCTGAGTCGATCCGCA
TGCATCCCGATCAGGAAACCCTGAAAGGTATGATGGGGGCTGCCGGCTTTGACAACGTGACCTATTTTAACCTGACCGGC
GGTATTGTGGCCCTGCATCGCGGCTTCAAGTTCTGA

Upstream 100 bases:

>100_bases
CGCTCCGCATCGCAGGCATGACAGCGGCTGCGAGGTGGGGTATTGCGTAGGGTTCTGGTACACTCATCCACACTAAATTG
ACTGAATAGCAGGCAGGACA

Downstream 100 bases:

>100_bases
TATGGACATGCCGATGCTGTTAACCCCGTTGTTGACCGGCGTGCTGGAAACATCGCTCAACAGCCTGCTGTTCCGCGATC
GCAGCATGAAAGCCGCCCGT

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MADQPQETTDFGFRTVARDEKQAMVADVFHSVAAKYDVMNDLMSFGIHRIWKRFTIDCSGVRRGQRVLDLAGGTGDLAAK
FSRMVGEQGQVVLADINDSMLKMGREKLRDRGIIGNVNYVQANAEALPFPDNYFDCITISFGLRNVTDKDKALRSMFRVL
KPGGRLLVLEFSKPLLAPLSKAYDAYSFHVLPKIGELVVKDPESYRYLAESIRMHPDQETLKGMMGAAGFDNVTYFNLTG
GIVALHRGFKF

Sequences:

>Translated_251_residues
MADQPQETTDFGFRTVARDEKQAMVADVFHSVAAKYDVMNDLMSFGIHRIWKRFTIDCSGVRRGQRVLDLAGGTGDLAAK
FSRMVGEQGQVVLADINDSMLKMGREKLRDRGIIGNVNYVQANAEALPFPDNYFDCITISFGLRNVTDKDKALRSMFRVL
KPGGRLLVLEFSKPLLAPLSKAYDAYSFHVLPKIGELVVKDPESYRYLAESIRMHPDQETLKGMMGAAGFDNVTYFNLTG
GIVALHRGFKF
>Mature_250_residues
ADQPQETTDFGFRTVARDEKQAMVADVFHSVAAKYDVMNDLMSFGIHRIWKRFTIDCSGVRRGQRVLDLAGGTGDLAAKF
SRMVGEQGQVVLADINDSMLKMGREKLRDRGIIGNVNYVQANAEALPFPDNYFDCITISFGLRNVTDKDKALRSMFRVLK
PGGRLLVLEFSKPLLAPLSKAYDAYSFHVLPKIGELVVKDPESYRYLAESIRMHPDQETLKGMMGAAGFDNVTYFNLTGG
IVALHRGFKF

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2)

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family

Homologues:

Organism=Homo sapiens, GI116063536, Length=276, Percent_Identity=46.0144927536232, Blast_Score=232, Evalue=2e-61,
Organism=Escherichia coli, GI48994983, Length=251, Percent_Identity=82.0717131474104, Blast_Score=439, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17557039, Length=249, Percent_Identity=44.578313253012, Blast_Score=215, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6323526, Length=265, Percent_Identity=41.8867924528302, Blast_Score=180, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24641702, Length=259, Percent_Identity=45.1737451737452, Blast_Score=233, Evalue=9e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIE_SERP5 (A8G8B8)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001476486.1
- ProteinModelPortal:   A8G8B8
- STRING:   A8G8B8
- GeneID:   5606558
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0248
- eggNOG:   COG2226
- HOGENOM:   HBG559580
- OMA:   FDVYTIA
- ProtClustDB:   PRK00216
- BioCyc:   SPRO399741:SPRO_0248-MONOMER
- HAMAP:   MF_01813
- InterPro:   IPR004033
- PANTHER:   PTHR10108:SF24
- TIGRFAMs:   TIGR01934

Pfam domain/function: PF01209 Ubie_methyltran

EC number: 2.1.1.-

Molecular weight: Translated: 28087; Mature: 27956

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS01183 UBIE_1; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADQPQETTDFGFRTVARDEKQAMVADVFHSVAAKYDVMNDLMSFGIHRIWKRFTIDCSG
CCCCCCCCHHHCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VRRGQRVLDLAGGTGDLAAKFSRMVGEQGQVVLADINDSMLKMGREKLRDRGIIGNVNYV
HHCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCEEE
QANAEALPFPDNYFDCITISFGLRNVTDKDKALRSMFRVLKPGGRLLVLEFSKPLLAPLS
EECCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHH
KAYDAYSFHVLPKIGELVVKDPESYRYLAESIRMHPDQETLKGMMGAAGFDNVTYFNLTG
HHHCCCEEEECCCCCHHEEECCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEECC
GIVALHRGFKF
CHHHHHCCCCC
>Mature Secondary Structure 
ADQPQETTDFGFRTVARDEKQAMVADVFHSVAAKYDVMNDLMSFGIHRIWKRFTIDCSG
CCCCCCCHHHCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VRRGQRVLDLAGGTGDLAAKFSRMVGEQGQVVLADINDSMLKMGREKLRDRGIIGNVNYV
HHCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCEEE
QANAEALPFPDNYFDCITISFGLRNVTDKDKALRSMFRVLKPGGRLLVLEFSKPLLAPLS
EECCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHH
KAYDAYSFHVLPKIGELVVKDPESYRYLAESIRMHPDQETLKGMMGAAGFDNVTYFNLTG
HHHCCCEEEECCCCCHHEEECCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEECC
GIVALHRGFKF
CHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA