Click here to switch to the map view.

The map label for this gene is wza

Identifier: 157158902

GI number: 157158902

Start: 2319209

End: 2320348

Strand: Reverse

Name: wza

Synonym: EcE24377A_2355

Alternate gene names: 157158902

Gene position: 2320348-2319209 (Counterclockwise)

Preceding gene: 157155494

Following gene: 157158537

Centisome position: 46.6

GC content: 53.25

Gene sequence:

>1140_bases
ATGATGAAATCCAAAATGAAATTGATGCCATTATTGGTGTCAGTAACCTTGATAAGCGGTTGCACAGTACTTCCGGGCAG
CAATATGTCGACGATGGGCAAAGACGTCATCAAACAGCAGGACGCTGATTTCGATCTCGACAAAATGGTGAATGTTTATC
CGTTGACCCCGCGCCTGATTGATCAATTACGTCCACGACCCAACGTAGCGCGCCCCAATATGACGCTGGAAAGTGAGATC
GCGAATTACCAGTATCGCGTCGGGCCGGGGGACGTTCTGAATGTCACCGTCTGGGATCACCCGGAACTCACCACGCCAGC
CGGTCAGTACCGCAGCTCCAGTGACACCGGCAACTGGGTACAGCCTGACGGCACTATGTTTTACCCGTATATCGGCAAGG
TTCACGTAGTCGGGAAAACGCTCGCTGAAATCCGCAGTGATATTACCGGGCGCTTAGCGACGTACATCGCTGACCCGCAG
GTGGACGTTAATATCGCCGCCTTCCGCTCACAAAAGGCCTATATCTCCGGACAGGTGAATAAATCCGGTCAACAGGCGAT
CACCAACGTACCACTGACCATTCTCGACGCCATCAACGCCGCAGGTGGCCTGACCGACACCGCTGACTGGCGCAACGTGG
TGCTAACACACAATGGTCGTGAAGAACGCATTTCTTTGCAGGCGCTGATGCAAAACGGCGACCTCAATCAGAACCGACTG
CTTTATCCCGGCGATATTCTCTACGTGCCGCGTAATGATGATCTGAAAGTGTTTGTGATGGGTGAAGTGAAGAAACAGAG
CACCCTGAAAATGGACTTTAGCGGCATGACCCTGACTGAAGCCCTGGGCAATGCCGAAGGTATCGACATGACCACCTCCA
ACGCCAGCGGCATCTTTGTCATTCGTCCGCTGAAAGGCGAGGGCGGGCGTAATGGCAAGATTGCCAATATCTACCAGCTG
GATATGTCCGATGCGACGTCGCTGGTGATGGCGACAGAATTCCGCCTGCAACCTTATGACGTGGTGTATGTCACCACCGC
CCCGGTTTCCCGCTGGAACCGTCTGATCAATCAGTTGCTGCCAACTATTAGCGGTGTCCGTTACATGACGGATACAGCCA
GCGACATTCATAACTGGTAA

Upstream 100 bases:

>100_bases
AGTTAAATTTGTATTCAATTAACTGCAAGGTAAATGTGCATGGCGACATTGCTATTAATAGTGCACAGGATAATTACTCT
GCCAAAGTGATAAATAAACA

Downstream 100 bases:

>100_bases
TCGTCATGTTTAACAACATCTTAGTTGTCTGTGTCGGCAATATTTGCCGTTCCCCGACGGCGGAACGCTTACTGCAACGT
TATCACCCGGAGCTGAAAGT

Product: polysaccharide biosynthesis/export protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADFDLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEI
ANYQYRVGPGDVLNVTVWDHPELTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHNGREERISLQALMQNGDLNQNRL
LYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGMTLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQL
DMSDATSLVMATEFRLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW

Sequences:

>Translated_379_residues
MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADFDLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEI
ANYQYRVGPGDVLNVTVWDHPELTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHNGREERISLQALMQNGDLNQNRL
LYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGMTLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQL
DMSDATSLVMATEFRLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW
>Mature_379_residues
MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADFDLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEI
ANYQYRVGPGDVLNVTVWDHPELTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHNGREERISLQALMQNGDLNQNRL
LYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGMTLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQL
DMSDATSLVMATEFRLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW

Specific function: Probably involved in the export of the extracellular polysaccharide colanic acid from the cell to medium

COG id: COG1596

COG function: function code M; Periplasmic protein involved in polysaccharide export

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BexD/CtrA/VexA family

Homologues:

Organism=Escherichia coli, GI1788376, Length=379, Percent_Identity=100, Blast_Score=780, Evalue=0.0,
Organism=Escherichia coli, GI1787218, Length=379, Percent_Identity=64.1160949868074, Blast_Score=518, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): WZA_ECO57 (P0A931)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C90987
- PIR:   F85832
- RefSeq:   NP_288568.1
- RefSeq:   NP_310894.1
- ProteinModelPortal:   P0A931
- SMR:   P0A931
- EnsemblBacteria:   EBESCT00000026690
- EnsemblBacteria:   EBESCT00000059598
- GeneID:   913884
- GeneID:   956750
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3227
- KEGG:   ecs:ECs2867
- GeneTree:   EBGT00050000009572
- HOGENOM:   HBG505123
- OMA:   NWVHSDG
- ProtClustDB:   PRK15078
- BioCyc:   ECOL83334:ECS2867-MONOMER
- InterPro:   IPR003715
- InterPro:   IPR019554

Pfam domain/function: PF02563 Poly_export; PF10531 SLBB

EC number: NA

Molecular weight: Translated: 41911; Mature: 41911

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADFDLDKMVNVYPLTPRLI
CCCCHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHCCCCCCHHHCCEEEECCHHHH
DQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPELTTPAGQYRSSSDTGNWV
HHCCCCCCCCCCCCEEHHHHCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEE
QPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQVDVNIAAFRSQKAYISGQVN
CCCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEECCCCEEEEECCC
KSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHNGREERISLQALMQNGDLNQNRL
CCCHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCEE
LYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGMTLTEALGNAEGIDMTTSNASGIFV
ECCCCEEEEECCCCEEEEEEECCCCCCEEEEECCCCCHHHHHCCCCCCEEECCCCCCEEE
IRPLKGEGGRNGKIANIYQLDMSDATSLVMATEFRLQPYDVVYVTTAPVSRWNRLINQLL
EEECCCCCCCCCCEEEEEEECCCCCCEEEEEEEEECCCEEEEEEEECCHHHHHHHHHHHH
PTISGVRYMTDTASDIHNW
HHHCCCEEEECCHHHHCCC
>Mature Secondary Structure
MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADFDLDKMVNVYPLTPRLI
CCCCHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHCCCCCCHHHCCEEEECCHHHH
DQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPELTTPAGQYRSSSDTGNWV
HHCCCCCCCCCCCCEEHHHHCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEE
QPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQVDVNIAAFRSQKAYISGQVN
CCCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEECCCCEEEEECCC
KSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHNGREERISLQALMQNGDLNQNRL
CCCHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCEE
LYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGMTLTEALGNAEGIDMTTSNASGIFV
ECCCCEEEEECCCCEEEEEEECCCCCCEEEEECCCCCHHHHHCCCCCCEEECCCCCCEEE
IRPLKGEGGRNGKIANIYQLDMSDATSLVMATEFRLQPYDVVYVTTAPVSRWNRLINQLL
EEECCCCCCCCCCEEEEEEECCCCCCEEEEEEEEECCCEEEEEEEECCHHHHHHHHHHHH
PTISGVRYMTDTASDIHNW
HHHCCCEEEECCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796