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The map label for this gene is insQ [H]

Identifier: 157158236

GI number: 157158236

Start: 2137538

End: 2138701

Strand: Reverse

Name: insQ [H]

Synonym: EcE24377A_2166

Alternate gene names: 157158236

Gene position: 2138701-2137538 (Counterclockwise)

Preceding gene: 157157515

Following gene: 157155931

Centisome position: 42.95

GC content: 51.12

Gene sequence:

>1164_bases
GTGAAACGACTACAGGCATTTAAATTCCAGTTAAGACCCGGTGGTCAACAGGAGCGTGAAATGAGGCGCTTCGCAGGCGC
ATGTCGTTTCGTTTTCAATCGTGCTCTGGCACGTCAGAATGAGAATCATGAGGCCGGTAATAAATACATCCCTTACGGGA
AAATGGCTTCCTGGCTGGTTGAGTGGAAAAATGCCACTGAAACGCAATGGCTTAAAGATGCCCCGTCACAGCCATTGCAA
CAGTCACTGAAAGACCTTGAGCGGGCTTACAAAAACTTCTTCCAGAATCGGGCTGCTTTTCCCCGATTCAAAAAGCGGGG
ACAGAATGATGTATTCCGCTACCCGCAGGGCGTTAAGCTCGATCAGGAAAACAGCCGTATTTTTCTGCCGAAACTGGGCT
GGATGCGCTACCGGAACAGCCGTCAGGTCACGGGTGTTGTGAAAAATGTCACTGTCAGCCAGTCCTGCGGTAAGTGGTAC
ATCAGTATTCAGACAGAAAGTGAAGTATCAACTCCGGTTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGC
TAAACTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGAAGCTGGCGAGAC
TTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCC
TGTATCGCAAATATCCGCAGGGACTACCTTCACAAAGTCACAACGACCGTCAGCAAAAACCACGCAATGATAGTCATTGA
GGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAG
GTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCCAGCTTGCGTACAAGCAGCTCTGGCGTGGCGGT
CAGGTGCTTGCTGTTCCGCCAGCGTACACAAGCCAGCGTTGCGCGTGCTGTGGTCATACAGCGAAAGAAAACCGCCTGTC
ACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAACGGCGCTCGTAACATTTTAGCGGCGG
GGCACGCCGTTCTTGCCTGTGGAGGATGTCAACAAACAGGTTGA

Upstream 100 bases:

>100_bases
TCTGGTGACAAAGACCAGGTGAACATGCATCAGGAAAACACAATGCCTTCCACGCCAGATATCTGTTTCTTTTTTCACAG
ACCAAAGTATCATTTGGCCT

Downstream 100 bases:

>100_bases
ATTAACTCCGCGCCCTGGTTTTTGACATTACCTACCGCGCGCGATACCGGGTGCCAGGAGAACTGGTTTGCTGGCACACA
GCCACTGGCCGCGATTTCTG

Product: IS605 family transposase OrfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHEAGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQ
QSLKDLERAYKNFFQNRAAFPRFKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQKRKIQRLHS
CIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGG
QVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGGCQQTG

Sequences:

>Translated_387_residues
MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHEAGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQ
QSLKDLERAYKNFFQNRAAFPRFKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQKRKIQRLHS
CIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGG
QVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGGCQQTG
>Mature_387_residues
MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHEAGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQ
QSLKDLERAYKNFFQNRAAFPRFKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQKRKIQRLHS
CIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGG
QVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGGCQQTG

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG0675

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transposase 35 family [H]

Homologues:

Organism=Escherichia coli, GI288549314, Length=367, Percent_Identity=97.275204359673, Blast_Score=745, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001959
- InterPro:   IPR010095
- InterPro:   IPR021027 [H]

Pfam domain/function: PF12323 HTH_14; PF01385 Transposase_2; PF07282 Transposase_35 [H]

EC number: NA

Molecular weight: Translated: 43982; Mature: 43982

Theoretical pI: Translated: 11.01; Mature: 11.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHEAGNKYIPYGKMASWLV
CCCCHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
EWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQNRAAFPRFKKRGQNDVFRYPQGVKL
HHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHCCCCCCEE
DQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTESEVSTPVHPSASMV
CCCCCEEEEECCHHHHHCCCCHHHHHHHHCEECCCCCEEEEEEEECCCCCCCCCCCCCEE
GLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQKRKIQRLHS
ECHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
CIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSIL
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHH
DQGWYEMRRQLAYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTA
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHCCCCHHHHHHHHHHCEEEEECEEC
NADVNGARNILAAGHAVLACGGCQQTG
CCCCCHHHHHHHCCCHHEEECCCCCCC
>Mature Secondary Structure
MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHEAGNKYIPYGKMASWLV
CCCCHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
EWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQNRAAFPRFKKRGQNDVFRYPQGVKL
HHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHCCCCCCEE
DQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTESEVSTPVHPSASMV
CCCCCEEEEECCHHHHHCCCCHHHHHHHHCEECCCCCEEEEEEEECCCCCCCCCCCCCEE
GLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQKRKIQRLHS
ECHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
CIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSIL
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHH
DQGWYEMRRQLAYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTA
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHCCCCHHHHHHHHHHCEEEEECEEC
NADVNGARNILAAGHAVLACGGCQQTG
CCCCCHHHHHHHCCCHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]