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The map label for this gene is rfbC [H]

Identifier: 157158061

GI number: 157158061

Start: 2292512

End: 2293060

Strand: Reverse

Name: rfbC [H]

Synonym: EcE24377A_2329

Alternate gene names: 157158061

Gene position: 2293060-2292512 (Counterclockwise)

Preceding gene: 157157449

Following gene: 157156098

Centisome position: 46.05

GC content: 39.71

Gene sequence:

>549_bases
ATGAACGTAATTAAAACTGAAATTCCAGACGTACTGATTTTTGAACCGAAAGTTTTTGGTGATGAGCGTGGTTTCTTTTT
TGAGAGCTTTAACCAGAAGGTTTTTGAGGAAGCTGTAGGCCGCAAAGTTGAATTTGTCCAGGATAACCATTCGAAGTCTT
GTAAAGGTGTCTTACGCGGATTGCATTATCAGTTAGAACCTTATGCACAAGGGAAATTGGTGCGCTGCGTTGTTGGTGAA
GTTTTTGATGTTGCGGTTGATATTCGTAAATCGTCACCAACTTTTGGCAAATGGGTTGGGGTGAATTTATCTGCTGAGAA
CAAGCGCCAGTTGTGGATACCTGAAGGATTTGCACATGGTTTTTTGGTGCTGAGTGAGACTGCGGAGTTTGTATATAAAA
CTACAAATTATTATCATCCGGAAAGTGATAGAGGGATCCGATGGGATGATGAGGAATTATCTATTACGTGGCCTAGAGGA
AATGAACTAAACTTATCAAATAAAGATCAGCAACATCCGAGCTTTCAAAACTGTCAGTGTTTTGAATAA

Upstream 100 bases:

>100_bases
CGGTTTATTGATGCTGAGCAGGTGAAAGTATTAGCCGAACCGCTGAAGAAAAATGCTTATGGTCAGTATCTGCTCAAAAT
GATTAAAGGTTATTAATAAA

Downstream 100 bases:

>100_bases
CTGTTTCGAGGAGATATTTTATGACGTAAAATATCTCCTAATAATTGGTTAGGTATTGATAAATACAATGTCTCGCAAAA
ATAATTCTGTTGACATAGTG

Product: dTDP-4-dehydrorhamnose 3,5-epimerase

Products: NA

Alternate protein names: Thymidine diphospho-4-keto-rhamnose 3,5-epimerase; dTDP-4-keto-6-deoxyglucose 3,5-epimerase; dTDP-6-deoxy-D-xylo-4-hexulose 3,5-epimerase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVEFVQDNHSKSCKGVLRGLHYQLEPYAQGKLVRCVVGE
VFDVAVDIRKSSPTFGKWVGVNLSAENKRQLWIPEGFAHGFLVLSETAEFVYKTTNYYHPESDRGIRWDDEELSITWPRG
NELNLSNKDQQHPSFQNCQCFE

Sequences:

>Translated_182_residues
MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVEFVQDNHSKSCKGVLRGLHYQLEPYAQGKLVRCVVGE
VFDVAVDIRKSSPTFGKWVGVNLSAENKRQLWIPEGFAHGFLVLSETAEFVYKTTNYYHPESDRGIRWDDEELSITWPRG
NELNLSNKDQQHPSFQNCQCFE
>Mature_182_residues
MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVEFVQDNHSKSCKGVLRGLHYQLEPYAQGKLVRCVVGE
VFDVAVDIRKSSPTFGKWVGVNLSAENKRQLWIPEGFAHGFLVLSETAEFVYKTTNYYHPESDRGIRWDDEELSITWPRG
NELNLSNKDQQHPSFQNCQCFE

Specific function: Catalyzes the epimerization of the C3' and C5'positions of dTDP-6-deoxy-D-xylo-4-hexulose, forming dTDP-6-deoxy-L-lyxo-4- hexulose [H]

COG id: COG1898

COG function: function code M; dTDP-4-dehydrorhamnose 3,5-epimerase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose 3,5-epimerase family [H]

Homologues:

Organism=Escherichia coli, GI1788350, Length=171, Percent_Identity=70.1754385964912, Blast_Score=249, Evalue=8e-68,
Organism=Caenorhabditis elegans, GI17550412, Length=174, Percent_Identity=51.7241379310345, Blast_Score=167, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR000888
- InterPro:   IPR014710
- ProDom:   PD001462 [H]

Pfam domain/function: PF00908 dTDP_sugar_isom [H]

EC number: =5.1.3.13 [H]

Molecular weight: Translated: 21058; Mature: 21058

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVEFVQDNHSKSCKGVLRG
CCCCCCCCCCEEEECCEECCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHH
LHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVNLSAENKRQLWIPEGFAHG
HCEEECCCCCCCEEEEHHHHHHHHEEEEECCCCCCCEEEEEEECCCCCEEEECCCCCCCE
FLVLSETAEFVYKTTNYYHPESDRGIRWDDEELSITWPRGNELNLSNKDQQHPSFQNCQC
EEEEEHHHHHHHHCCCEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCC
FE
CC
>Mature Secondary Structure
MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVEFVQDNHSKSCKGVLRG
CCCCCCCCCCEEEECCEECCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHH
LHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVNLSAENKRQLWIPEGFAHG
HCEEECCCCCCCEEEEHHHHHHHHEEEEECCCCCCCEEEEEEECCCCCEEEECCCCCCCE
FLVLSETAEFVYKTTNYYHPESDRGIRWDDEELSITWPRGNELNLSNKDQQHPSFQNCQC
EEEEEHHHHHHHHCCCEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCC
FE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8170390; 8157605; 12384590; 12704152 [H]