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The map label for this gene is gnd [H]

Identifier: 157157026

GI number: 157157026

Start: 2282520

End: 2283926

Strand: Reverse

Name: gnd [H]

Synonym: EcE24377A_2320

Alternate gene names: 157157026

Gene position: 2283926-2282520 (Counterclockwise)

Preceding gene: 157158578

Following gene: 157154758

Centisome position: 45.87

GC content: 50.04

Gene sequence:

>1407_bases
ATGTCAAAGCAACAGATTGGCGTAGTCGGTATGGCAGTGATGGGGCGCAACCTTGCGCTCAACATTGAAAGCCGTGGTTA
TACCGTCTCTATTTTCAACCGTTCCCGTGAGAAAACGGAAGAAGTGATTGCCGAAAATCCAGACAAAAAACTGGTTCCTT
ACTATACGGTGAAAGAGTTCGTTGAATCTCTGGAAACGCCTCGTCGCATCCTGTTAATGGTGAAAGCAGGTGCAGGCACG
GATGCTGCTATTGATTCCCTCAAGCCATACCTCGATAAAGGTGACATCATCATTGATGGAGGTAACACCTTCTTCCAGGA
CACCATTCGTCGTAACCGTGAGCTTTCTGCCGAAGGCTTTAACTTCATTGGTACCGGTGTCTCCGGTGGTGAAGAAGGCG
CGCTGAAAGGTCCTTCCATTATGCCTGGTGGACAGAAAGAAGCCTATGAACTGGTTGCGCCGATCCTGACCAAAATCGCC
GCAGTGGCTGAAGACGGTGAGCCATGCGTTACCTATATTGGTGCCGATGGTGCAGGTCACTATGTGAAGATGGTTCACAA
CGGTATTGAATACGGCGATATGCAGCTGATTGCTGAAGCCTATTCTCTGCTTAAAGGTGGCCTGAATCTCACCAACGAAG
AACTGGCGCAGACCTTTACCGAGTGGAATAACGGTGAACTGAGCAGCTACCTTATCGACATCACCAAAGATATCTTCACC
AAAAAAGATGAAGACGGTAACTACCTGGTTGATGTGATCCTGGATGAAGCGGCTAACAAAGGTACCGGTAAATGGACCAG
CCAGAGCGCACTGGATCTCGGCGAACCGCTGTCGCTGATTACCGAGTCTGTGTTTGCACGTTATATCTCTTCTCTGAAAG
ATCAGCGTGTTGCCGCGTCTAAAGTTCTCTCTGGCCCGCAAGCGCAGCCAGCTGGCGACAAGGCTGAGTTCATCGAAAAA
GTTCGTCGTGCTCTGTATCTGGGCAAAATCGTTTCTTACGCCCAAGGCTTCTCTCAGCTGCGTGCTGCGTCTGAAGAGTA
CAACTGGGATCTGAACTACGGCGAAATCGCGAAGATTTTCCGTGCTGGCTGCATCATCCGTGCGCAGTTCCTGCAGAAAA
TCACTGATGCATATGCCGAAAATCCGCAGATCGCTAACCTGCTGCTGGCTCCGTACTTTAAACAAATTGCCGATGACTAT
CAGCAGGCGCTGCGTGATGTTGTTGCTTATGCAGTACAGAACGGTATCCCGGTTCCCACCTTCGCCGCTGCGGTTGCCTA
TTATGACAGCTACCGTGCCGCTGTTCTGCCTGCGAACCTGATCCAGGCACAGCGTGACTATTTCGGTGCACATACTTATA
AGCGCATTGATAAAGAAGGTGTGTTCCATACTGAATGGCTGGATTAA

Upstream 100 bases:

>100_bases
CATTTAAATTATGACTTTTATTCACGAAATCTACGTTCTAATACATAGAACTATCTGAACTATTATTTATGATAGTTTCT
CACCCTGACAGGAGTAAACA

Downstream 100 bases:

>100_bases
TCTGACTTAACCAACAATAAATTGAGGCCCGGCATATATTGCTGGGCTTTTTTTGCCAATAATCTTATAGACTAAATTTC
ACTGCTTAATATTAACTTAA

Product: 6-phosphogluconate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIAENPDKKLVPYYTVKEFVESLETPRRILLMVKAGAGT
DAAIDSLKPYLDKGDIIIDGGNTFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQTFTEWNNGELSSYLIDITKDIFT
KKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEPLSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEK
VRRALYLGKIVSYAQGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPYFKQIADDY
QQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGAHTYKRIDKEGVFHTEWLD

Sequences:

>Translated_468_residues
MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIAENPDKKLVPYYTVKEFVESLETPRRILLMVKAGAGT
DAAIDSLKPYLDKGDIIIDGGNTFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQTFTEWNNGELSSYLIDITKDIFT
KKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEPLSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEK
VRRALYLGKIVSYAQGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPYFKQIADDY
QQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGAHTYKRIDKEGVFHTEWLD
>Mature_467_residues
SKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIAENPDKKLVPYYTVKEFVESLETPRRILLMVKAGAGTD
AAIDSLKPYLDKGDIIIDGGNTFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIAA
VAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQTFTEWNNGELSSYLIDITKDIFTK
KDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEPLSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKV
RRALYLGKIVSYAQGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPYFKQIADDYQ
QALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGAHTYKRIDKEGVFHTEWLD

Specific function: Catalyzes the oxidative decarboxylation of 6- phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH [H]

COG id: COG0362

COG function: function code G; 6-phosphogluconate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 6-phosphogluconate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40068518, Length=469, Percent_Identity=53.9445628997868, Blast_Score=494, Evalue=1e-140,
Organism=Escherichia coli, GI1788341, Length=468, Percent_Identity=99.5726495726496, Blast_Score=954, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17542558, Length=471, Percent_Identity=50.1061571125265, Blast_Score=490, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6321695, Length=459, Percent_Identity=50.1089324618736, Blast_Score=467, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6321977, Length=452, Percent_Identity=50.2212389380531, Blast_Score=448, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24639279, Length=470, Percent_Identity=51.2765957446809, Blast_Score=463, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR006114
- InterPro:   IPR006113
- InterPro:   IPR006115
- InterPro:   IPR006184
- InterPro:   IPR013328
- InterPro:   IPR012284
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF00393 6PGD; PF03446 NAD_binding_2 [H]

EC number: =1.1.1.44 [H]

Molecular weight: Translated: 51524; Mature: 51393

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00461 6PGD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIAENPDKKLVPYYTVKEF
CCCCHHHHHHHHHHCCCEEEEEECCCCEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHH
VESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGNTFFQDTIRRNRELSAEGF
HHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCCCCCC
NFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIAAVAEDGEPCVTYIGADGAGH
CEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCH
YVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQTFTEWNNGELSSYLIDITKDIFT
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHC
KKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEPLSLITESVFARYISSLKDQRVAAS
CCCCCCCEEEEEEEHHHCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYAQGFSQLRAASEEYNWDLNYGEIAKIF
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
RAGCIIRAQFLQKITDAYAENPQIANLLLAPYFKQIADDYQQALRDVVAYAVQNGIPVPT
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
FAAAVAYYDSYRAAVLPANLIQAQRDYFGAHTYKRIDKEGVFHTEWLD
HHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIAENPDKKLVPYYTVKEF
CCCHHHHHHHHHHCCCEEEEEECCCCEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHH
VESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGNTFFQDTIRRNRELSAEGF
HHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCCCCCC
NFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIAAVAEDGEPCVTYIGADGAGH
CEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCH
YVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQTFTEWNNGELSSYLIDITKDIFT
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHC
KKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEPLSLITESVFARYISSLKDQRVAAS
CCCCCCCEEEEEEEHHHCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYAQGFSQLRAASEEYNWDLNYGEIAKIF
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
RAGCIIRAQFLQKITDAYAENPQIANLLLAPYFKQIADDYQQALRDVVAYAVQNGIPVPT
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
FAAAVAYYDSYRAAVLPANLIQAQRDYFGAHTYKRIDKEGVFHTEWLD
HHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7515042 [H]