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The map label for this gene is manB [H]

Identifier: 157155689

GI number: 157155689

Start: 2304532

End: 2305902

Strand: Reverse

Name: manB [H]

Synonym: EcE24377A_2340

Alternate gene names: 157155689

Gene position: 2305902-2304532 (Counterclockwise)

Preceding gene: 157157123

Following gene: 157157993

Centisome position: 46.31

GC content: 54.7

Gene sequence:

>1371_bases
ATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGGAAATTAGGCGAAGAACTGAATGAAGATATCGCCTGGCG
CATTGGTCGCGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTGTTAGGCGGTGATGTCCGTCTCACCAGCGAAACCT
TAAAACTGGCGCTGGCAAAAGGTTTACAGGATGCGGGCGTTGACGTGCTGGATATCGGTATGTCCGGCACCGAAGAGATC
TATTTCGCCACGTTCCATCTCGGCGTGGATGGCGGCATTGAAGTCACCGCCAGCCATAATCCGATGGATTATAACGGCAT
GAAGCTGGTGCGCGAAGGGGCTCGCCCGATCAGCGGTGATACCGGACTGCGCGACGTCCAGCGTCTGGCTGAAGCCAACG
ACTTTCCTCCCGTCGATGAAACCAAACGCGGTCGCTATCAGCAAATCAACCTGCGTGACGCTTACGTTGATCACCTGTTC
GGTTATATCAATGTCAAAAATCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAATGGCGCAGCGGGTCCGGTGGTGGA
CGCCATTGAAGCCCGCTTTAAAGCCCTCGGCGCGCCCGTGGAATTAATCAAAGTACACAACACACCGGACGGCAATTTCC
CCAACGGTATTCCTAACCCGCTGCTACCGGAATGTCGCGACGACACCCGCAATGCGGTCATCAAACACGGCGCGGATATG
GGCATTGCCTTTGATGGCGATTTTGACCGCTGTTTCCTGTTTGACGAAAAAGGGCAGTTTATCGAGGGCTATTACATTGT
CGGCCTGCTGGCAGAAGCATTCCTCGAAAAAAATCCCGGCGCGAAGATCATCCACGATCCACGTCTCTCCTGGAACACCG
TTGATGTGGTGACTGCCGCAGGTGGCACGCCGGTAATGTCGAAAACCGGACACGCCTTTCTTAAAGAACGTATGCGCAAG
GAGGACGCCATCTACGGTGGCGAAATGAGCGCTCACCATTACTTCCGTGATTTCGCTTACTGCGACAGCGGCATGATCCC
GTGGCTACTGGTAACCGAACTGGTGTGCCTGAAAGAGAAAACGCTGGGCGAACTGGTACGCGACCGGATGGTGGCGTTTC
CGGCAAGCGGTGAGATCAACAGCAAACTGGCGCAACCCGCTGAGGCGATTAACCGCGTGGAACAGCATTTTAGCCGTGAG
GCGCTGGCGGTGGATCGTACCGATGGCATCAGTATGACCTTTGCCGACTGGCGTTTTAACCTGCGCTCCTCCAACACCGA
ACCGGTGGTGCGGTTGAATGTGGAATCGCGCGGTGATGTGCCGCTGATGGAAGCGCGAACGCGAACTCTGCTGACGTTGC
TGAACGAGTAA

Upstream 100 bases:

>100_bases
CGCATCAGGCAATGAATACGAAACCGAGATGTAAATAACGACAAAAACAAAATTGGCCGTTTCGGTCAGGGCCAACTATT
GCCTGAAAAAGGGTAACGAC

Downstream 100 bases:

>100_bases
TGTCGGATCTTCCCTTACCCCACTGCGGGTAAGGGGCTAATAACAGGAACAACGATGACAAATCTAAAAAAGCGCGAGCG
AGCGAAAACCAATGCATCGT

Product: phosphomannomutase

Products: NA

Alternate protein names: PMM [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLGGDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEI
YFATFHLGVDGGIEVTASHNPMDYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIPNPLLPECRDDTRNAVIKHGADM
GIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLEKNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFLKERMRK
EDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVTELVCLKEKTLGELVRDRMVAFPASGEINSKLAQPAEAINRVEQHFSRE
ALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTLLTLLNE

Sequences:

>Translated_456_residues
MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLGGDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEI
YFATFHLGVDGGIEVTASHNPMDYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIPNPLLPECRDDTRNAVIKHGADM
GIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLEKNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFLKERMRK
EDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVTELVCLKEKTLGELVRDRMVAFPASGEINSKLAQPAEAINRVEQHFSRE
ALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTLLTLLNE
>Mature_456_residues
MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLGGDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEI
YFATFHLGVDGGIEVTASHNPMDYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIPNPLLPECRDDTRNAVIKHGADM
GIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLEKNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFLKERMRK
EDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVTELVCLKEKTLGELVRDRMVAFPASGEINSKLAQPAEAINRVEQHFSRE
ALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTLLTLLNE

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS O antigen [H]

COG id: COG1109

COG function: function code G; Phosphomannomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphohexose mutase family [H]

Homologues:

Organism=Escherichia coli, GI1788361, Length=456, Percent_Identity=98.6842105263158, Blast_Score=931, Evalue=0.0,
Organism=Escherichia coli, GI1789566, Length=457, Percent_Identity=26.4770240700219, Blast_Score=85, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005844
- InterPro:   IPR016055
- InterPro:   IPR005845
- InterPro:   IPR005846
- InterPro:   IPR005843
- InterPro:   IPR016066
- InterPro:   IPR005841 [H]

Pfam domain/function: PF02878 PGM_PMM_I; PF02879 PGM_PMM_II; PF02880 PGM_PMM_III; PF00408 PGM_PMM_IV [H]

EC number: =5.4.2.8 [H]

Molecular weight: Translated: 50493; Mature: 50493

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00710 PGM_PMM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLGGDVRLTSETLKLALAK
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECHHHHHHHHHH
GLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPMDYNGMKLVREGARPISGD
CHHHCCCCEEEECCCCCCEEEEEEEEEECCCCEEEEECCCCCCCCHHHHHHHCCCCCCCC
TGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLFGYINVKNLTPLKLVINSGNG
CCHHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHHEEEECCCCEEEEEEECCCC
AAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIPNPLLPECRDDTRNAVIKHGADM
CCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCC
GIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLEKNPGAKIIHDPRLSWNTVDVVTAA
EEEECCCCCEEEEECCCCCEECHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEEEC
GGTPVMSKTGHAFLKERMRKEDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVTELVCLKEK
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
TLGELVRDRMVAFPASGEINSKLAQPAEAINRVEQHFSREALAVDRTDGISMTFADWRFN
HHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCEEEEEEEEEE
LRSSNTEPVVRLNVESRGDVPLMEARTRTLLTLLNE
ECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLGGDVRLTSETLKLALAK
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECHHHHHHHHHH
GLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPMDYNGMKLVREGARPISGD
CHHHCCCCEEEECCCCCCEEEEEEEEEECCCCEEEEECCCCCCCCHHHHHHHCCCCCCCC
TGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLFGYINVKNLTPLKLVINSGNG
CCHHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHHEEEECCCCEEEEEEECCCC
AAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIPNPLLPECRDDTRNAVIKHGADM
CCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCC
GIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLEKNPGAKIIHDPRLSWNTVDVVTAA
EEEECCCCCEEEEECCCCCEECHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEEEC
GGTPVMSKTGHAFLKERMRKEDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVTELVCLKEK
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
TLGELVRDRMVAFPASGEINSKLAQPAEAINRVEQHFSREALAVDRTDGISMTFADWRFN
HHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCEEEEEEEEEE
LRSSNTEPVVRLNVESRGDVPLMEARTRTLLTLLNE
ECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9673232; 10222209; 11206551; 11258796 [H]