Click here to switch to the map view.

The map label for this gene is thiM

Identifier: 157155537

GI number: 157155537

Start: 2366015

End: 2366803

Strand: Reverse

Name: thiM

Synonym: EcE24377A_2392

Alternate gene names: 157155537

Gene position: 2366803-2366015 (Counterclockwise)

Preceding gene: 157155925

Following gene: 157154867

Centisome position: 47.53

GC content: 54.12

Gene sequence:

>789_bases
ATGCAAGTCGACCTGCTGAGTTCAGCGCAATCTGCGCACGCGTTACACCTTTTTCACCAACATTCCCCTCTTGTGCACTG
CATGACCAACGATGTGGTGCAAACCTTTACTGCCAATACCTTGCTGGTGCTCGGTGCATCGCCAGCGATGGTTATCGAAA
CCGAAGAGGCCAGTCAGTTTGCGGCTATTGCCAGTGCGTTGCTGATTAACGTTGGCACGCTGACGCAGCTATGCGCACAG
TCGATGTGTGCCGCCGTTGAGCAAGCAAAAAGCTCTCAAACACCCTGGACGCTTGATCCAGTAGCGGTTGGTGCACTCGA
TTATCGCCGCCGTTTTTGTCTGGAACTTCTGTCTCATAAGCCAACCGCCATACGTGGTAATGCTTCGGAAATCATGGCAT
TAGCTGGCATTGCTAATGGTGGACGGGGAGTGGATACCACTGACGCCGCAGCTAACGCGATACCCGCTGCACAAACACTG
GCACGGGAAACTGGCGCAATCGTCGTGGTCACTGGCGAGATGGATTATGTTACCGATGGACATCGTATCATTGGTATTCA
CGGTGGCGATCCGTTAATGACCAAAGTGGTAGGAACTGGCTGTGCATTATCGGCGGTTGTCGCTGCCTGCTGTGCGTTAC
CAGGCGATACGCTGGAAAATGTCGCATCTGCCTGTCACTGGATGAAACAAGCCGGAGAGCGCGCAGTCGCCAGAAGCGAG
GGGCCAGGCAGTTTTGTTCCACATTTCCTTGATGCGCTCTGGCAATTGACGCAGGAGGTGCAGGCATGA

Upstream 100 bases:

>100_bases
GGCTGAGAAATACCCGTATCACCTGATCTGGATAATGCCAGCGTAGGGAAGTCACGGACCACCAGGTCATTGCTTCTTCA
CGTTATGGCAGGAGCAAACT

Downstream 100 bases:

>100_bases
AACGAATTAACGCTCTGACGATTGCCGGTACTGATCCCAGTGGTGGTGCGGGAATTCAGGCCGATCTTAAAACCTTCTCG
GCACTTGGCGCTTATGGTTG

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLVLGASPAMVIETEEASQFAAIASALLINVGTLTQLCAQ
SMCAAVEQAKSSQTPWTLDPVAVGALDYRRRFCLELLSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTLENVASACHWMKQAGERAVARSE
GPGSFVPHFLDALWQLTQEVQA

Sequences:

>Translated_262_residues
MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLVLGASPAMVIETEEASQFAAIASALLINVGTLTQLCAQ
SMCAAVEQAKSSQTPWTLDPVAVGALDYRRRFCLELLSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTLENVASACHWMKQAGERAVARSE
GPGSFVPHFLDALWQLTQEVQA
>Mature_262_residues
MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLVLGASPAMVIETEEASQFAAIASALLINVGTLTQLCAQ
SMCAAVEQAKSSQTPWTLDPVAVGALDYRRRFCLELLSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTLENVASACHWMKQAGERAVARSE
GPGSFVPHFLDALWQLTQEVQA

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=262, Percent_Identity=96.1832061068702, Blast_Score=488, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6325042, Length=271, Percent_Identity=32.8413284132841, Blast_Score=109, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_ECO24 (A7ZNS3)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001463452.1
- ProteinModelPortal:   A7ZNS3
- SMR:   A7ZNS3
- STRING:   A7ZNS3
- EnsemblBacteria:   EBESCT00000018715
- GeneID:   5590734
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_2392
- NMPDR:   fig|331111.3.peg.134
- eggNOG:   COG2145
- GeneTree:   EBGT00050000010804
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- ProtClustDB:   PRK09355
- BioCyc:   ECOL331111:ECE24377A_2392-MONOMER
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099
- TIGRFAMs:   TIGR00694

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 27339; Mature: 27339

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: BINDING 50-50 BINDING 125-125 BINDING 171-171 BINDING 198-198

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLVLGASPAMVIETEEASQF
CCCCCHHCCHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCEEEEEECCCEEEEECCHHHHH
AAIASALLINVGTLTQLCAQSMCAAVEQAKSSQTPWTLDPVAVGALDYRRRFCLELLSHK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHCC
PTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTLARETGAIVVVTGEMDYVTDG
CCEECCCHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHCCEEEEEECCCCEEECC
HRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTLENVASACHWMKQAGERAVARSE
CEEEEEECCCHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
GPGSFVPHFLDALWQLTQEVQA
CCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLVLGASPAMVIETEEASQF
CCCCCHHCCHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCEEEEEECCCEEEEECCHHHHH
AAIASALLINVGTLTQLCAQSMCAAVEQAKSSQTPWTLDPVAVGALDYRRRFCLELLSHK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHCC
PTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTLARETGAIVVVTGEMDYVTDG
CCEECCCHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHCCEEEEEECCCCEEECC
HRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTLENVASACHWMKQAGERAVARSE
CEEEEEECCCHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
GPGSFVPHFLDALWQLTQEVQA
CCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA