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The map label for this gene is arcA

Identifier: 157163849

GI number: 157163849

Start: 4641476

End: 4642192

Strand: Reverse

Name: arcA

Synonym: EcHS_A4637

Alternate gene names: 157163849

Gene position: 4642192-4641476 (Counterclockwise)

Preceding gene: NA

Following gene: 157163844

Centisome position: 99.97

GC content: 50.91

Gene sequence:

>717_bases
ATGCAGACCCCGCACATTCTTATCGTTGAAGACGAGTTGGTAACACGCAACACGTTGAAAAGTATTTTCGAAGCGGAAGG
CTATGATGTTTTCGAAGCGACAGATGGCGCGGAAATGCATCAGATCCTCTCTGAATATGACATCAACCTGGTGATCATGG
ATATCAATCTGCCGGGTAAGAACGGTCTTCTGTTAGCGCGTGAACTGCGCGAGCAGGCGAATGTTGCGTTGATGTTCCTG
ACTGGCCGTGACAACGAAGTCGATAAAATTCTCGGCCTCGAAATCGGTGCAGATGACTACATCACCAAACCGTTCAACCC
GCGTGAACTGACGATTCGTGCACGCAACCTGCTGTCCCGTACCATGAATCTGGGTACTGTCAGCGAAGAACGTCGTAGCG
TTGAAAGCTACAAGTTCAATGGTTGGGAACTGGACATCAACAGCCGTTCGTTGATCGGCCCTGATGGCGAGCAGTACAAG
CTGCCGCGCAGCGAGTTCCGCGCCATGCTTCACTTCTGTGAAAACCCAGGCAAAATTCAGTCTCGTGCTGAACTGCTGAA
GAAAATGACCGGCCGTGAGCTGAAACCACACGACCGTACTGTAGACGTGACGATCCGCCGTATTCGTAAACATTTCGAAT
CTACGCCGGATACGCCGGAAATCATCGCCACCATCCACGGTGAAGGTTATCGCTTCTGTGGTGATCTGGAAGATTAA

Upstream 100 bases:

>100_bases
GTCATGTTACGCCGATCATGTTAATTTGCAGCATGCATCAGGCAGGTCAGGGACTTTTGTACTTCCTGTTTCGATTTAGT
TGGCAATTTAGGTAGCAAAC

Downstream 100 bases:

>100_bases
TCGGCTTTACCACCGTCAAAAAAAACGGCGCTTTTTAGCGCCGTTTTTATTTTTCAACCTTATTTCCAGATACGTAATTG
ATCGTCCATTGTAACTTCTT

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILSEYDINLVIMDINLPGKNGLLLARELREQANVALMFL
TGRDNEVDKILGLEIGADDYITKPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDTPEIIATIHGEGYRFCGDLED

Sequences:

>Translated_238_residues
MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILSEYDINLVIMDINLPGKNGLLLARELREQANVALMFL
TGRDNEVDKILGLEIGADDYITKPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDTPEIIATIHGEGYRFCGDLED
>Mature_238_residues
MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILSEYDINLVIMDINLPGKNGLLLARELREQANVALMFL
TGRDNEVDKILGLEIGADDYITKPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDTPEIIATIHGEGYRFCGDLED

Specific function: Member of the two-component regulatory system ArcB/ArcA. Represses a wide variety of aerobic enzymes under anaerobic conditions. It also may be involved in the osmoregulation of envelope proteins. When activated by ArcB, it negatively regulates the expres

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1790863, Length=238, Percent_Identity=100, Blast_Score=489, Evalue=1e-140,
Organism=Escherichia coli, GI1787229, Length=233, Percent_Identity=42.4892703862661, Blast_Score=190, Evalue=7e-50,
Organism=Escherichia coli, GI1789809, Length=233, Percent_Identity=36.0515021459227, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1786599, Length=231, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1788394, Length=233, Percent_Identity=31.7596566523605, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI2367329, Length=225, Percent_Identity=32.4444444444444, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1790860, Length=235, Percent_Identity=31.9148936170213, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI1786911, Length=229, Percent_Identity=31.8777292576419, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI87082012, Length=229, Percent_Identity=31.0043668122271, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI1787375, Length=228, Percent_Identity=30.2631578947368, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786784, Length=226, Percent_Identity=28.7610619469027, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI145693140, Length=232, Percent_Identity=28.448275862069, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1789402, Length=226, Percent_Identity=29.646017699115, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790552, Length=234, Percent_Identity=30.3418803418803, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1788550, Length=212, Percent_Identity=25.9433962264151, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ARCA_ECO57 (P0A9Q3)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A86140
- PIR:   G91298
- RefSeq:   NP_291014.1
- RefSeq:   NP_313386.1
- ProteinModelPortal:   P0A9Q3
- SMR:   P0A9Q3
- EnsemblBacteria:   EBESCT00000026596
- EnsemblBacteria:   EBESCT00000058901
- GeneID:   913476
- GeneID:   959654
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z6004
- KEGG:   ecs:ECs5359
- GeneTree:   EBGT00070000031686
- HOGENOM:   HBG753323
- OMA:   FEAEGYI
- ProtClustDB:   PRK11173
- BioCyc:   ECOL83334:ECS5359-MONOMER
- GO:   GO:0005737
- GO:   GO:0006350
- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- SMART:   SM00448
- SMART:   SM00862

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C; SSF46894 Bipartite_resp_reg_C-effector; SSF52172 CheY_like

EC number: NA

Molecular weight: Translated: 27292; Mature: 27292

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILSEYDINLVIMDINLPGK
CCCCCEEEEECCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCEEEEEEEECCCC
NGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYITKPFNPRELTIRARNLLSR
CCEEHHHHHHHHCCEEEEEEECCCCCCHHEEEEEECCCCCCCCCCCCCEEEHHHHHHHHH
TMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYKLPRSEFRAMLHFCENPGKIQ
HHCCCCCHHHHHHHHHEECCCEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCHH
SRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDTPEIIATIHGEGYRFCGDLED
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEECCCCCC
>Mature Secondary Structure
MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILSEYDINLVIMDINLPGK
CCCCCEEEEECCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCEEEEEEEECCCC
NGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYITKPFNPRELTIRARNLLSR
CCEEHHHHHHHHCCEEEEEEECCCCCCHHEEEEEECCCCCCCCCCCCCEEEHHHHHHHHH
TMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYKLPRSEFRAMLHFCENPGKIQ
HHCCCCCHHHHHHHHHEECCCEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCHH
SRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDTPEIIATIHGEGYRFCGDLED
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796