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The map label for this gene is serB

Identifier: 157163835

GI number: 157163835

Start: 4626886

End: 4627854

Strand: Direct

Name: serB

Synonym: EcHS_A4623

Alternate gene names: 157163835

Gene position: 4626886-4627854 (Clockwise)

Preceding gene: 157163832

Following gene: 157163836

Centisome position: 99.64

GC content: 56.14

Gene sequence:

>969_bases
ATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGA
AGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCC
AATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCA
GGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCT
GCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGG
CCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGC
CGTGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAAC
GCAACTGGTGCTCAAGCTGGAAACGCTGGGCTGGAAAGTGGCGATTGCCTCCGGCGGCTTTACTTTCTTTGCTGAATATC
TGCGCGACAAGCTGCGCCTGACCGCCGTGGTAGCCAATGAACTGGAGATCATGGACGGTAAATTTACCGGCAATGTGATC
GGCGACATCGTAGACGCGCAGTACAAAGCGAAAACTTTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGAC
CGTGGCGATTGGCGATGGAGCTAATGACCTGCCGATGATCAAAGCAGCAGGGCTGGGGATTGCTTACCATGCCAAGCCAA
AAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCTGGCAGCCTGAAT
CAGAAGTAA

Upstream 100 bases:

>100_bases
ACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATGCGTTATTTTCCCTGCTTCGA
ACGATTTTACAGGAGCCTTA

Downstream 100 bases:

>100_bases
TTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTG
TAATGAATGCGGGGCCGATT

Product: phosphoserine phosphatase

Products: NA

Alternate protein names: PSP; PSPase; O-phosphoserine phosphohydrolase

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYGRGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLA
GSLTARATRLAHEAQLDVAPLGKIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKLRLTAVVANELEIMDGKFTGNVI
GDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGANDLPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLN
QK

Sequences:

>Translated_322_residues
MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYGRGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLA
GSLTARATRLAHEAQLDVAPLGKIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKLRLTAVVANELEIMDGKFTGNVI
GDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGANDLPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLN
QK
>Mature_321_residues
PNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYGRGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAG
SLTARATRLAHEAQLDVAPLGKIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRSR
VATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKLRLTAVVANELEIMDGKFTGNVIG
DIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGANDLPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQ
K

Specific function: Unknown

COG id: COG0560

COG function: function code E; Phosphoserine phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serB family

Homologues:

Organism=Homo sapiens, GI46249388, Length=182, Percent_Identity=31.8681318681319, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1790849, Length=322, Percent_Identity=100, Blast_Score=657, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534454, Length=236, Percent_Identity=29.6610169491525, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321647, Length=247, Percent_Identity=38.4615384615385, Blast_Score=162, Evalue=8e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SERB_ECO57 (P0AGB1)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B91297
- RefSeq:   NP_291001.1
- RefSeq:   NP_313373.1
- ProteinModelPortal:   P0AGB1
- SMR:   P0AGB1
- EnsemblBacteria:   EBESCT00000025251
- EnsemblBacteria:   EBESCT00000058437
- GeneID:   913496
- GeneID:   959667
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5989
- KEGG:   ecs:ECs5346
- GeneTree:   EBGT00050000011712
- HOGENOM:   HBG645339
- OMA:   DYQVVRL
- ProtClustDB:   PRK11133
- BioCyc:   ECOL83334:ECS5346-MONOMER
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006383
- InterPro:   IPR023190
- InterPro:   IPR004469
- Gene3D:   G3DSA:3.40.50.1000
- Gene3D:   G3DSA:1.10.150.210
- TIGRFAMs:   TIGR01488
- TIGRFAMs:   TIGR00338

Pfam domain/function: PF00702 Hydrolase; SSF56784 SSF56784

EC number: =3.1.3.3

Molecular weight: Translated: 35043; Mature: 34912

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: ACT_SITE 116-116 ACT_SITE 118-118 BINDING 125-125 BINDING 161-161 BINDING 249-249 BINDING 275-275

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYGRGLDKQRLTQYQSKLG
CCCCEECCCCCCCCCCCCCCEECCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHH
AAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLGKIPHLRTPGLLVMDMDST
HHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCC
AIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRSRVATLKGADANILQQVRENL
EEEEEEHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCC
PLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKLRLTAVVANELEIMDGKFTGNVI
CCCCCHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHH
GDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGANDLPMIKAAGLGIAYHAKPKVNEKAE
HHHHCCHHHHHHHHHHHHHHCCCHHHEEEECCCCCCCCEEEECCCEEEEECCCCCCCCEE
VTIRHADLMGVFCILSGSLNQK
EEEEHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYGRGLDKQRLTQYQSKLG
CCCEECCCCCCCCCCCCCCEECCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHH
AAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLGKIPHLRTPGLLVMDMDST
HHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCC
AIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRSRVATLKGADANILQQVRENL
EEEEEEHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCC
PLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKLRLTAVVANELEIMDGKFTGNVI
CCCCCHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHH
GDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGANDLPMIKAAGLGIAYHAKPKVNEKAE
HHHHCCHHHHHHHHHHHHHHCCCHHHEEEECCCCCCCCEEEECCCEEEEECCCCCCCCEE
VTIRHADLMGVFCILSGSLNQK
EEEEHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796