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The map label for this gene is hpaH [H]

Identifier: 157163796

GI number: 157163796

Start: 4589205

End: 4590008

Strand: Reverse

Name: hpaH [H]

Synonym: EcHS_A4580

Alternate gene names: 157163796

Gene position: 4590008-4589205 (Counterclockwise)

Preceding gene: 157163797

Following gene: 157163795

Centisome position: 98.85

GC content: 58.46

Gene sequence:

>804_bases
ATGTTCGATAAACATACCCACACCCTGATCGCCCAGCGTCTGGATCAGGCAGAAAAACAGCGCGAACAGATCCGCGCGAT
CTCGCTGGATTACCCGGAGATCACCATCGAAGACGCTTACGCGGTGCAGCGTGAATGGGTTCGCCTGAAAATAGCCGAAG
GTCGCACGCTGAAAGGCCACAAAATCGGCCTGACCTCGAAAGCGATGCAGGCCAGCTCACAAATCAGCGAGCCGGATTAC
GGTGCCCTGCTGGATGACATGTTCTTCCACGATGGCAGCGATATCCCGACCGATCGCTTTATCGTGCCGCGCATTGAAGT
GGAGCTGGCTTTCGTGCTGGCAAAACCGCTGCGTGGGCCAAACTGCACGCTGTTCGACGTCTACAACGCCACGGACTACG
TGATCCCGGCGCTGGAGCTGATCGACGCTCGCTGCCACAACATCGATCCGGAAACCCAGCGCCCGCGCAAAGTGTTCGAC
ACCATTTCCGATAACGCCGCCAACGCCGGGGTGATCCTCGGCGGTCGTCCGATCAAACCAGACGAACTGGATCTGCGTTG
GATCTCCGCCCTGATGTATCGCAATGGCGTGATTGAAGAAACCGGCGTCGCCGCTGGTGTGCTGAATCATCCGGCAAACG
GCGTGGCCTGGCTGGCGAACAAACTCGCACCGTATGACGTACAACTGGAAGCCGGGCAAATCATTCTCGGCGGTTCGTTC
ACCCGCCCGGTTCCGGCGCGTAAGGGCGACACCTTCCACGTCGATTACGGCAACATGGGCTCCATTAGCTGCCGCTTTGT
TTAA

Upstream 100 bases:

>100_bases
GCCCGGTGGCGCTGCGCTTACCGGGCCTACAAAACCCCAAACCGTACACCGTAGGCCGGATAAGGCGCAGCCGCATCCGG
CAATGCCACAGGATATCGCT

Downstream 100 bases:

>100_bases
GGAGAGAACGATGGAAAACAGTTTTAAAGCGGCGCTGAAAGCAGGCCGTCCGCAGATTGGATTATGGCTGGGGCTGAGCA
GCAGCTACAGCGCGGAGTTA

Product: 2-oxo-hepta-3-ene-1,7-dioic acid hydratase

Products: NA

Alternate protein names: OHED hydratase [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQREWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDY
GALLDDMFFHDGSDIPTDRFIVPRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWLANKLAPYDVQLEAGQIILGGSF
TRPVPARKGDTFHVDYGNMGSISCRFV

Sequences:

>Translated_267_residues
MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQREWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDY
GALLDDMFFHDGSDIPTDRFIVPRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWLANKLAPYDVQLEAGQIILGGSF
TRPVPARKGDTFHVDYGNMGSISCRFV
>Mature_267_residues
MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQREWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDY
GALLDDMFFHDGSDIPTDRFIVPRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWLANKLAPYDVQLEAGQIILGGSF
TRPVPARKGDTFHVDYGNMGSISCRFV

Specific function: Transforms 2-oxo-hept-3-ene-1,7-dioate (OHED) into 2,4- dihydoxy-hept-2-ene-1,7-dioic acid (HHED) [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=263, Percent_Identity=34.2205323193916, Blast_Score=149, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012690 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: 4.2.1.-

Molecular weight: Translated: 29715; Mature: 29715

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQREWVRLKIAEGRTLKGH
CCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEECHHHHHHHEEEEEEECCCCEECCC
KIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIVPRIEVELAFVLAKPLRGP
EECCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCEEECEEHEEEEEEECCCCCCC
NCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFDTISDNAANAGVILGGRPIKP
CEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEECCCCCCC
DELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWLANKLAPYDVQLEAGQIILGGSF
CCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHHHHHCCCCCEEEEECCEEEECCCC
TRPVPARKGDTFHVDYGNMGSISCRFV
CCCCCCCCCCEEEEECCCCCCEEEEEC
>Mature Secondary Structure
MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQREWVRLKIAEGRTLKGH
CCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEECHHHHHHHEEEEEEECCCCEECCC
KIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIVPRIEVELAFVLAKPLRGP
EECCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCEEECEEHEEEEEEECCCCCCC
NCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFDTISDNAANAGVILGGRPIKP
CEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEECCCCCCC
DELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWLANKLAPYDVQLEAGQIILGGSF
CCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHHHHHCCCCCEEEEECCEEEECCCC
TRPVPARKGDTFHVDYGNMGSISCRFV
CCCCCCCCCCEEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7737515 [H]