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The map label for this gene is hpaI [H]

Identifier: 157163795

GI number: 157163795

Start: 4588406

End: 4589194

Strand: Reverse

Name: hpaI [H]

Synonym: EcHS_A4579

Alternate gene names: 157163795

Gene position: 4589194-4588406 (Counterclockwise)

Preceding gene: 157163796

Following gene: 157163794

Centisome position: 98.83

GC content: 59.95

Gene sequence:

>789_bases
ATGGAAAACAGTTTTAAAGCGGCGCTGAAAGCAGGCCGTCCGCAGATTGGATTATGGCTGGGGCTGAGCAGCAGCTACAG
CGCGGAGTTACTGGCCGGAGCAGGATTCGACTGGTTGTTGATCGACGGTGAGCACGCGCCGAATAACGTGCAAACCGTGC
TCACCCAGCTACAGGCGATTGCGCCCTACCCCAGCCAGCCGGTAGTACGTCCGTCGTGGAACGATCCGGTGCAAATCAAA
CAACTGCTGGACGTCGGCACACAAACCTTACTGGTGCCGATGGTTCAAAACGCTGACGAAGCCCGTGAAGCGGTACGCGC
CACCCGTTATCCCCCCGCCGGTATTCGCGGTGTGGGCAGTGCGCTGGCCCGCGCCTCGCGCTGGAATCGTATTCCTGATT
ACCTGCAAAAAGCCAACGATCAAATGTGCGTGCTGGTGCAGATCGAAACGCGTGAGGCAATGAAGAACTTACCGCAGATT
CTGGACGTGGAAGGAGTCGACGGCGTGTTTATCGGCCCGGCGGATCTGAGCGCCGATATGGGTTATGCCGGTAATCCACA
GCACCCGGAAGTACAGGCCGCCATTGAGCAGGCGATCGTGCAGATCCGCGAAGCGGGCAAAGCACCGGGGATCCTGATCG
CCAATGAGCAACTGGCAAAACGCTATCTGGAACTGGGCGCGCTGTTTGTCGCCGTCGGCGTTGACACCACCCTGCTCGCC
CGCGCCGCTGAAGCGCTGGCAGCACGGTTTGGTGCGCAGGCTACCGCCGTGAAGCCCGGCGTGTATTAA

Upstream 100 bases:

>100_bases
TCGTTCACCCGCCCGGTTCCGGCGCGTAAGGGCGACACCTTCCACGTCGATTACGGCAACATGGGCTCCATTAGCTGCCG
CTTTGTTTAAGGAGAGAACG

Downstream 100 bases:

>100_bases
TGCCGGATGCGCTGCGCTTATCCGGCCTACACTCGCACCAAATCGTAGGCCGGATAAGGCGTTACGCCGCATCCAGCAAA
AACCTTGTCGTACCCTACAA

Product: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGEHAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIK
QLLDVGTQTLLVPMVQNADEAREAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQLAKRYLELGALFVAVGVDTTLLA
RAAEALAARFGAQATAVKPGVY

Sequences:

>Translated_262_residues
MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGEHAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIK
QLLDVGTQTLLVPMVQNADEAREAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQLAKRYLELGALFVAVGVDTTLLA
RAAEALAARFGAQATAVKPGVY
>Mature_262_residues
MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGEHAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIK
QLLDVGTQTLLVPMVQNADEAREAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQLAKRYLELGALFVAVGVDTTLLA
RAAEALAARFGAQATAVKPGVY

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788578, Length=250, Percent_Identity=56.4, Blast_Score=291, Evalue=4e-80,
Organism=Escherichia coli, GI1789514, Length=248, Percent_Identity=48.3870967741936, Blast_Score=234, Evalue=6e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 28057; Mature: 28057

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGEHAPNNVQTVLTQLQAI
CCCHHHHHHHCCCCCEEEEEECCCCCCHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHH
APYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAREAVRATRYPPAGIRGVGS
CCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHH
ALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQILDVEGVDGVFIGPADLSADM
HHHHHHHCCCCHHHHHHCCCCEEEEEEECHHHHHHCCHHHCCCCCCCEEEECCCCCCCCC
GYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQLAKRYLELGALFVAVGVDTTLLA
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCHHHHH
RAAEALAARFGAQATAVKPGVY
HHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGEHAPNNVQTVLTQLQAI
CCCHHHHHHHCCCCCEEEEEECCCCCCHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHH
APYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAREAVRATRYPPAGIRGVGS
CCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHH
ALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQILDVEGVDGVFIGPADLSADM
HHHHHHHCCCCHHHHHHCCCCEEEEEEECHHHHHHCCHHHCCCCCCCEEEECCCCCCCCC
GYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQLAKRYLELGALFVAVGVDTTLLA
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCHHHHH
RAAEALAARFGAQATAVKPGVY
HHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12384590; 12704152 [H]