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The map label for this gene is iraD [H]

Identifier: 157163772

GI number: 157163772

Start: 4561992

End: 4562393

Strand: Direct

Name: iraD [H]

Synonym: EcHS_A4554

Alternate gene names: 157163772

Gene position: 4561992-4562393 (Clockwise)

Preceding gene: 157163771

Following gene: 157163777

Centisome position: 98.24

GC content: 47.51

Gene sequence:

>402_bases
GTGCGCAAAATGATGCGCCAATCACTTCAGGCTGTTTTACCTGAAATTTCAGGCAATAAAACGTCATTGCTGCGTAAATC
GGTATGCAGCGATATCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCAGAGT
GGCAAGTGCATAACCAGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAG
CCACGGATTGCGGCGTTGCAGGTTAATTTTAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGCTGTA
CCACGACGGTGAACCGCTAACGTTTGATCTGGTCTGGGATAATGGCGGTTGGTGTAGTGCGATGCTGGAGAATGTCAGCT
AA

Upstream 100 bases:

>100_bases
ATGGATGTAATGAACAAAAGGGAATGGCTATGGAAAATGAGCATCAATACAGTGGTGCCCGGTGTTCAGGGCAAGCCGCA
TATGTTGCTAAACGTCAGGA

Downstream 100 bases:

>100_bases
GCACAATCTCCAGCTCGCGCAGTTCACGCCAGAAGCGTTCGGCAACTGGATTCATGCGGGTATTCATTCGGTATGCATAG
GCCTGAATCGGGATCACCAG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MRKMMRQSLQAVLPEISGNKTSLLRKSVCSDILTLFNSPHSALPSLLVSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHE
PRIAALQVNFKEAYCHTLAISLEIMLYHDGEPLTFDLVWDNGGWCSAMLENVS

Sequences:

>Translated_133_residues
MRKMMRQSLQAVLPEISGNKTSLLRKSVCSDILTLFNSPHSALPSLLVSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHE
PRIAALQVNFKEAYCHTLAISLEIMLYHDGEPLTFDLVWDNGGWCSAMLENVS
>Mature_133_residues
MRKMMRQSLQAVLPEISGNKTSLLRKSVCSDILTLFNSPHSALPSLLVSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHE
PRIAALQVNFKEAYCHTLAISLEIMLYHDGEPLTFDLVWDNGGWCSAMLENVS

Specific function: Inhibits RpoS proteolysis by regulating RssB activity, thereby increasing the stability of the sigma stress factor RpoS during oxidative stress. Its effect on RpoS stability is due to its interaction with RssB, which probably blocks the interaction of Rss

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GpW/Gp25 family. IraD subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082422, Length=130, Percent_Identity=94.6153846153846, Blast_Score=253, Evalue=2e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007048 [H]

Pfam domain/function: PF04965 GPW_gp25 [H]

EC number: NA

Molecular weight: Translated: 15173; Mature: 15173

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKMMRQSLQAVLPEISGNKTSLLRKSVCSDILTLFNSPHSALPSLLVSGMPEWQVHNQS
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEECCCC
DKHLQSWYCRQLRSALLFHEPRIAALQVNFKEAYCHTLAISLEIMLYHDGEPLTFDLVWD
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHEEEEEEEEECCCCEEEEEEEC
NGGWCSAMLENVS
CCCHHHHHHHCCC
>Mature Secondary Structure
MRKMMRQSLQAVLPEISGNKTSLLRKSVCSDILTLFNSPHSALPSLLVSGMPEWQVHNQS
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEECCCC
DKHLQSWYCRQLRSALLFHEPRIAALQVNFKEAYCHTLAISLEIMLYHDGEPLTFDLVWD
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHEEEEEEEEECCCCEEEEEEEC
NGGWCSAMLENVS
CCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA