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The map label for this gene is nanC

Identifier: 157163754

GI number: 157163754

Start: 4543788

End: 4544504

Strand: Reverse

Name: nanC

Synonym: EcHS_A4536

Alternate gene names: 157163754

Gene position: 4544504-4543788 (Counterclockwise)

Preceding gene: 157163755

Following gene: 157163753

Centisome position: 97.87

GC content: 43.1

Gene sequence:

>717_bases
ATGAAAAAGGCTAAAATACTTTCTGGCGTATTATTACTGTGCTTTTCGTCCCCATTAATTTCTCAGGCTGCGACACTGGA
CGTACGTGGTGGATATCGTAGTGGAAGCCACGCCTATGAGACTCGACTCAAAGTCAGTGAGGGATGGCAAAATGGATGGT
GGGCAAGCATGGAAAGTAATACCTGGAATACCATTCATGATAATAAAAAGGAAAATGCCGCACTCAATGATGTTCAGGTT
GAAGTTAATTACGCGATTAAACTTGATGATCAATGGACGGTGCGCCCGGGAATGTTAACGCATTTTAGCAGCAACGGCAC
ACGCTACGGACCCTACGTAAAACTGTCCTGGGACGCGACAAAAGATCTTAATTTTGGCATTCGCTATCGTTACGACTGGA
AAGCTTACCGACAACAAGACTTATCCGGTGATATGTCTCGTGATAACGTTCATCGTTGGGATGGATATGTCACTTACCAT
ATTAATAGTGATTTCACCTTCGCATGGCAAACGACGCTATACAGCAAACAGAACGATTATCGCTATGCAAACCATAAGAA
ATGGGCGACGGAAAATGCATTTGTTCTACAATACCATATGACGCCCGATATTACGCCATACATAGAATATGACTACCTGG
ACCGTCAGGGTGTTTACAACGGCAGAGATAATTTATCGGAAAACAGTTATCGCATTGGTGTGTCATTTAAACTGTAG

Upstream 100 bases:

>100_bases
AGACACTATAAGCCTGGCAAAAAAATATTACACAACATAAATGCTAATTGTTTATGCGGGCTTTGTATTGCTTTCTGTAT
CCTACAAATGAGTGAAATTT

Downstream 100 bases:

>100_bases
TAGACAGGAGACAGTCACAATGAATAAAACAATAACGGCGCTTGCTATCATGATGGCTTCATTTGCCGCAAACGCGTCTG
TATTACCTGAAACTCCTGTG

Product: oligogalacturonate-specific porin protein (KdgM)

Products: NA

Alternate protein names: Porin nanC

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLKVSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQV
EVNYAIKLDDQWTVRPGMLTHFSSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGVYNGRDNLSENSYRIGVSFKL

Sequences:

>Translated_238_residues
MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLKVSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQV
EVNYAIKLDDQWTVRPGMLTHFSSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGVYNGRDNLSENSYRIGVSFKL
>Mature_238_residues
MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLKVSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQV
EVNYAIKLDDQWTVRPGMLTHFSSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGVYNGRDNLSENSYRIGVSFKL

Specific function: Outer membrane channel protein allowing the entry of N- acetylneuraminic acid (Neu5Ac or sialic acid) into the bacteria. NanC proteins form high-conductance channels which are open at low membrane potentials and which have a weak anion selectivity

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oligogalacturonate-specific porin kdgM (TC 1.B.35) family. NanC subfamily

Homologues:

Organism=Escherichia coli, GI87082420, Length=238, Percent_Identity=100, Blast_Score=496, Evalue=1e-142,
Organism=Escherichia coli, GI1790307, Length=235, Percent_Identity=25.1063829787234, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NANC_ECO57 (P69857)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- RefSeq:   NP_290927.1
- RefSeq:   NP_313297.2
- ProteinModelPortal:   P69857
- SMR:   P69857
- EnsemblBacteria:   EBESCT00000026327
- EnsemblBacteria:   EBESCT00000055708
- GeneID:   913696
- GeneID:   959742
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5907
- KEGG:   ecs:ECs5270
- GeneTree:   EBGT00050000008772
- HOGENOM:   HBG416127
- OMA:   SSDMSRD
- ProtClustDB:   CLSK880894
- BioCyc:   ECOL83334:ECS5270-MONOMER
- InterPro:   IPR009331
- InterPro:   IPR001702
- Gene3D:   G3DSA:2.40.160.10

Pfam domain/function: PF06178 KdgM

EC number: NA

Molecular weight: Translated: 27888; Mature: 27888

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLKVSEGWQNGWWASMESN
CCCHHHHHHHHHHHHCCCHHCCEEEEEECCCCCCCCCEEEEEEEEECCCCCCEEEEECCC
TWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHFSSNGTRYGPYVKLSWDAT
CCCCCCCCCCCCCEEEEEEEEEEEEEEECCCEEECCCEEEEECCCCCEECCEEEEEECCC
KDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYHINSDFTFAWQTTLYSKQNDY
CCCCCCEEEEECHHHHHHHCCCCCCCCCCCEECCCEEEEEECCCEEEEEEEEEECCCCCC
RYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGVYNGRDNLSENSYRIGVSFKL
EECCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEC
>Mature Secondary Structure
MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLKVSEGWQNGWWASMESN
CCCHHHHHHHHHHHHCCCHHCCEEEEEECCCCCCCCCEEEEEEEEECCCCCCEEEEECCC
TWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHFSSNGTRYGPYVKLSWDAT
CCCCCCCCCCCCCEEEEEEEEEEEEEEECCCEEECCCEEEEECCCCCEECCEEEEEECCC
KDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYHINSDFTFAWQTTLYSKQNDY
CCCCCCEEEEECHHHHHHHCCCCCCCCCCCEECCCEEEEEECCCEEEEEEEEEECCCCCC
RYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGVYNGRDNLSENSYRIGVSFKL
EECCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796