Click here to switch to the map view.

The map label for this gene is nanM2 [H]

Identifier: 157163753

GI number: 157163753

Start: 4542662

End: 4543768

Strand: Reverse

Name: nanM2 [H]

Synonym: EcHS_A4535

Alternate gene names: 157163753

Gene position: 4543768-4542662 (Counterclockwise)

Preceding gene: 157163754

Following gene: 157163752

Centisome position: 97.85

GC content: 43.63

Gene sequence:

>1107_bases
ATGAATAAAACAATAACGGCGCTTGCTATCATGATGGCTTCATTTGCCGCAAACGCGTCTGTATTACCTGAAACTCCTGT
GCCATTTAAAAGTGGTACCGGAGCAATTGATAACGACACTGTCTACATTGGTTTAGGTAGCGCAGGTACGGCATGGTACA
AGCTGGATACACAGGCCAAAGATAAAAAATGGACAGCGTTAGCTGCATTCCCTGGTGGACCAAGAGATCAAGCAACCTCG
GCATTTATTGATGGCAATCTGTATGTGTTTGGCGGCATTGGCAAAAATAGCAAGGGCTTGACTCAGGTATTTAATGACGT
ACACAAATACAACCCCAAAACCAATAGTTGGGTTAAATTGATGTCGCATGCACCGATGGGCATGGCGGGTCATGTGACTT
TTGTACACAACGGCAAGGCTTATGTTACTGGCGGTGTTAACCAGAATATCTTCAATGGCTATTTTGAAGATCTCAACGAG
GCTGGAAAAGATTCAGCAACTATAGACAAGATCAATGCCCATTATTTTGACAAAAAAGCAGAAGATTATTTCTTCAATAA
GTTTCTGTTGTCTTTTGATCCCTCAACACAGCAATGGAGTTACGCTGGCGAATCTCCCTGGTACGGAACGGCTGGTGCGG
CGGTTGTGAATAAAGGTGATAAAACCTGGCTTATTAATGGCGAAGCCAAACCAGGATTGCGAACGGATGCCGTATTTGAA
CTTGATTTCACCGGTAATAATTTAAAATGGAATAAGCTTGCTCCCGTCTCATCACCAGATGGCGTCGCTGGCGGTTTTGC
GGGGATAAGCAATGATTCTCTTATATTTGCCGGAGGGGCCGGATTCAAAGGTTCACGAGAAAATTACCAGAACGGTAAGA
ACTATGCGCATGAAGGCCTAAAAAAATCATATAGCACTGATATTCATCTTTGGCATAACGGGAAATGGGATAAATCGGGT
GAATTATCGCAAGGTCGGGCCTACGGAGTATCATTGCCCTGGAATAATAGTCTATTGATTATTGGCGGTGAAACTGCAGG
CGGCAAAGCGGTGACGGATTCAGTTTTGATCTCTGTGAAGGATAATAAAGTCACAGTACAAAATTAA

Upstream 100 bases:

>100_bases
CTGGACCGTCAGGGTGTTTACAACGGCAGAGATAATTTATCGGAAAACAGTTATCGCATTGGTGTGTCATTTAAACTGTA
GTAGACAGGAGACAGTCACA

Downstream 100 bases:

>100_bases
CGCTTCAGGGCCCCGGTAAAGGGGCCCTGCTATCAATTTGTCATTTCAATTACGCGAAATTTATATGAACGCAATAATAT
CGCCCGATTATTACTATGTT

Product: kelch repeat-containing protein

Products: NA

Alternate protein names: N-acetylneuraminate mutarotase 2; Neu5Ac mutarotase 2; Sialic acid epimerase 2 [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIGLGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATS
AFIDGNLYVFGGIGKNSKGLTQVFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
AGKDSATIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNKGDKTWLINGEAKPGLRTDAVFE
LDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIFAGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSG
ELSQGRAYGVSLPWNNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN

Sequences:

>Translated_368_residues
MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIGLGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATS
AFIDGNLYVFGGIGKNSKGLTQVFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
AGKDSATIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNKGDKTWLINGEAKPGLRTDAVFE
LDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIFAGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSG
ELSQGRAYGVSLPWNNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN
>Mature_368_residues
MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIGLGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATS
AFIDGNLYVFGGIGKNSKGLTQVFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
AGKDSATIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNKGDKTWLINGEAKPGLRTDAVFE
LDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIFAGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSG
ELSQGRAYGVSLPWNNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN

Specific function: Converts alpha-N-acetylneuranimic acid (Neu5Ac) to the beta-anomer, accelerating the equilibrium between the alpha- and beta-anomers. Probably facilitates sialidase-negative bacteria to compete sucessfully for limited amounts of extracellular Neu5Ac, whic

COG id: COG3055

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 7 Kelch repeats [H]

Homologues:

Organism=Escherichia coli, GI87082419, Length=368, Percent_Identity=98.9130434782609, Blast_Score=741, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015915
- InterPro:   IPR006652
- InterPro:   IPR019936 [H]

Pfam domain/function: PF01344 Kelch_1 [H]

EC number: NA

Molecular weight: Translated: 39557; Mature: 39557

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIGLGSAGTAWYKLDTQAK
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCC
DKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSKGLTQVFNDVHKYNPKTNSWVKL
CCCEEEEEECCCCCCCCCCEEEECCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHH
MSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNEAGKDSATIDKINAHYFDKKA
HHCCCCCEEEEEEEEECCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCHHCCCCCC
EDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNKGDKTWLINGEAKPGLRTDAVFE
HHHEEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCCCCCEEEE
LDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIFAGGAGFKGSRENYQNGKNYAHEGL
EEECCCCEEECEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHCCCHHHHHHHH
KKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPWNNSLLIIGGETAGGKAVTDSVLISVK
HHCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCEEECEEEEEEE
DNKVTVQN
CCEEEECC
>Mature Secondary Structure
MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIGLGSAGTAWYKLDTQAK
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCC
DKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSKGLTQVFNDVHKYNPKTNSWVKL
CCCEEEEEECCCCCCCCCCEEEECCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHH
MSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNEAGKDSATIDKINAHYFDKKA
HHCCCCCEEEEEEEEECCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCHHCCCCCC
EDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNKGDKTWLINGEAKPGLRTDAVFE
HHHEEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCCCCCEEEE
LDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIFAGGAGFKGSRENYQNGKNYAHEGL
EEECCCCEEECEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHCCCHHHHHHHH
KKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPWNNSLLIIGGETAGGKAVTDSVLISVK
HHCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCEEECEEEEEEE
DNKVTVQN
CCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]