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The map label for this gene is melB [H]

Identifier: 157163586

GI number: 157163586

Start: 4367390

End: 4368811

Strand: Direct

Name: melB [H]

Synonym: EcHS_A4361

Alternate gene names: 157163586

Gene position: 4367390-4368811 (Clockwise)

Preceding gene: 157163585

Following gene: 157163592

Centisome position: 94.05

GC content: 45.85

Gene sequence:

>1422_bases
ATGAGCATTTCAATGACTACAAAACTCAGTTATGGATTTGGAGCGTTCGGGAAGGATTTTGCGATCGGCATTGTGTATAT
GTACCTCATGTATTACTACACCGATGTCGTCGGGCTGTCTGTGGGTTTGGTCGGTACTTTGTTTCTGGTGGCGAGGATCT
GGGATGCTATTAACGATCCGATTATGGGATGGATTGTAAATGCTACGCGATCGCGATGGGGTAAGTTCAAACCCTGGATC
CTGATCGGTACGTTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCGCATCTGTTTGAAGGTACTACTCAGATTGT
CTTTGTTTGCGTGACCTACATCCTCTGGGGCATGACTTACACCATTATGGATATTCCCTTCTGGTCGCTGGTTCCAACCA
TCACGCTCGATAAACGTGAGCGCGAACAACTGGTTCCTTATCCGCGTTTTTTTGCCAGTCTGGCAGGCTTTGTTACGGCA
GGTGTGACGCTACCATTTGTTAATTATGTCGGCGGTGGCGATCGGGGATTTGGCTTTCAGATGTTCACTCTGGTACTGAT
CGCCTTTTTTATTGTTTCAACCATCATCACTCTGCGCAATGTGCATGAAGTCTTTTCGTCAGACAATCAACCGTCTGCTG
AAGGAAGCCATCTGACACTTAAAGCCATCGTTGCGCTAATTTATAAAAACGATCAGCTTTCATGCCTCTTGGGTATGGCT
CTTGCTTATAATGTAGCCAGCAACATTATTACCGGATTTGCTATCTATTATTTCTCATATGTTATCGGTGATGCGGATTT
GTTCCCCTATTATCTGTCGTATGCGGGAGCTGCTAACCTGGTGACGTTAGTATTCTTCCCACGCTTAGTTAAATCATTAT
CCCGACGCATTTTATGGGCCGGAGCATCTATTCTTCCGGTGTTAAGCTGTGGTGTTCTCCTGTTAATGGCATTAATGAGC
TATCACAACGTCGTCCTCATTGTGATTGCGGGTATTTTGCTGAATGTGGGAACGGCGCTTTTCTGGGTATTACAGGTCAT
CATGGTGGCAGATACCGTTGATTACGGTGAATATAAACTGCACGTACGCTGTGAAAGTATCGCTTACTCCGTGCAGACTA
TGGTGGTGAAGGGCGGTTCAGCCTTTGCGGCTTTTTTCATTGCGGTTGTGTTAGGGATGATTGGCTATGTACCGAATGTT
GAACAGTCTACGCAAGCCCTATTAGGTATGCAGTTTATTATGATTGCTCTACCAACTCTGTTTTTCATGGTAACGCTGAT
TCTCTACTTCCGTTTCTATCGCCTCAATGGCGACACGCTGCGCAGGATCCAGATTCATCTGCTGGATAAATATCGCAAAG
TACCGCCCGAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGA

Upstream 100 bases:

>100_bases
CGCGACTAAACGCTACTGCGCCGGGGGATTTATTCCGGCGCACACCTCTGACGATACCAATAACAGAAGGCGGGCGTTGG
TAACAGCGACCCGATACCCT

Downstream 100 bases:

>100_bases
CGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAA
ATTTTGTAGACCGGATAAGG

Product: melibiose:sodium symporter

Products: Li (I) [Cytoplasm]; melibiose [Cytoplasm]; Na (I) [Cytoplasm]; melibiose [Cytoplasm]; Proton [Cytoplasm]; melibiose [Cytoplasm] [C]

Alternate protein names: Melibiose permease; Melibiose transporter; Na+ (Li+)/melibiose symporter; Thiomethylgalactoside permease II [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWI
LIGTLANSVILFLLFSAHLFEGTTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHLTLKAIVALIYKNDQLSCLLGMA
LAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMS
YHNVVLIVIAGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGYVPNV
EQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDVKA

Sequences:

>Translated_473_residues
MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWI
LIGTLANSVILFLLFSAHLFEGTTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHLTLKAIVALIYKNDQLSCLLGMA
LAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMS
YHNVVLIVIAGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGYVPNV
EQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDVKA
>Mature_472_residues
SISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWIL
IGTLANSVILFLLFSAHLFEGTTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTAG
VTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHLTLKAIVALIYKNDQLSCLLGMAL
AYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSY
HNVVLIVIAGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGYVPNVE
QSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDVKA

Specific function: Responsible for melibiose transport. It is capable of using hydrogen, sodium, and lithium cations as coupling cations for cotransport, depending on the particular sugar transported (symport system) [H]

COG id: COG2211

COG function: function code G; Na+/melibiose symporter and related transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:galactoside symporter (TC 2.A.2) family [H]

Homologues:

Organism=Escherichia coli, GI1790561, Length=469, Percent_Identity=99.7867803837953, Blast_Score=944, Evalue=0.0,
Organism=Escherichia coli, GI87082306, Length=469, Percent_Identity=28.1449893390192, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1786466, Length=467, Percent_Identity=27.8372591006424, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI48994989, Length=448, Percent_Identity=26.1160714285714, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI145693206, Length=457, Percent_Identity=26.0393873085339, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1787902, Length=465, Percent_Identity=27.741935483871, Blast_Score=142, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016196
- InterPro:   IPR001927
- InterPro:   IPR018043 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 52625; Mature: 52494

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS00872 NA_GALACTOSIDE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDP
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEGTTQIVFVCVTYILWGMTY
HHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
TIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTAGVTLPFVNYVGGGDRGFGFQ
HHHHCCHHHHCCCEEECCHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHH
MFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHLTLKAIVALIYKNDQLSCLLGMA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
LAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANLVTLVFFPRLVKSLSRRILWA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHC
GASILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGTALFWVLQVIMVADTVDYGEYKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
HVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGYVPNVEQSTQALLGMQFIMIALPTL
EEEHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
FFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDVKA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDP
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC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HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Li (I) [Periplasm]; melibiose [Periplasm]; Na (I) [Periplasm]; melibiose [Periplasm]; Proton [Periplasm]; melibiose [Periplasm] [C]

Specific reaction: Li (I) [Periplasm] + melibiose [Periplasm] = Li (I) [Cytoplasm] + melibiose [Cytoplasm] Na (I) [Periplasm] + melibiose [Periplasm] = Na (I) [Cytoplasm] + melibiose [Cytoplasm] Proton [Periplasm] + melibiose [Periplasm] = Proton [Cytoplasm] + melibiose [Cy

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6323466; 7610040; 9278503; 1730719; 8672452; 1872836 [H]