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The map label for this gene is aceK [H]

Identifier: 157163482

GI number: 157163482

Start: 4247850

End: 4249586

Strand: Direct

Name: aceK [H]

Synonym: EcHS_A4251

Alternate gene names: 157163482

Gene position: 4247850-4249586 (Clockwise)

Preceding gene: 157163481

Following gene: 157163484

Centisome position: 91.48

GC content: 53.14

Gene sequence:

>1737_bases
ATGCCGCGTGGCCTGGAATTACTGATTGCTCAAACCATTTTGCAAGGCTTCGACGCTCAGTATGGTCGATTCCTCGAAGT
GACTTCCGGGGCGCAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTT
ACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTG
CGCGTTAAAGAGCATTACACGCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTA
CTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTA
CCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTG
CCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATTCATTACATCATTCGCCATCTGACGGAAACGCTGGGGACAGA
CAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTGGGCAAACTGA
TCACGCCTTCCGGCACGTTGCCCTTCTTACTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTG
ACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATGCACCGCTGCCCGCAGCGCT
GGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACTGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCA
AAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGA
ATGGTGATGTTGGTATTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAAGTTCGCGCCGCAGAAAGA
GATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGACCGTGTGGGCCGAATGGCGGACACCCAGG
AGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTCCAGGAAGCAGCGGAAAAA
ATCACCGATCTTGGTGAGCAGATTGTAATTCGCCATCTCTATATCGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGA
ACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTT
TTCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGGTTTTTTATGATTACGATGAAATTTGC
TACATGACGGAAGTGAATTTCCGCGACATCCCGCCGCCGCGCTATCCGGAAGACGAACTTGCCAGCGAACCGTGGTACAG
CGTCTCGCCGGGCGATGTTTTCCCGGAAGAGTTTCGCCACTGGCTATGCGCCGACCCGCGTATTGGTCCGCTGTTTGAAG
AGATGCACGCCGACCTGTTCCGCGCTGATTACTGGCGCGCACTACAAAACCGCATACGTGAAGGGCATGTGGAAGATGTT
TATGCGTATCGGCGCAGGCAAAGATTTAGCGTACGGTATGGGGAGATGCTTTTTTGA

Upstream 100 bases:

>100_bases
GCTTGCGCGTCTTATCATGCCTACAACCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCT
GCTCCTGATGAGGGCGCTAA

Downstream 100 bases:

>100_bases
GTAAAGCTTCCATATAATTTTTCTCCGCAATGTATCGAGGGTTATCCGTAAAGCCAAAGCTTTCAGCCATCTTATTTAAT
GTATTAAGGATTAATTCAGC

Product: bifunctional isocitrate dehydrogenase kinase/phosphatase protein

Products: NA

Alternate protein names: IDH kinase/phosphatase; IDHK/P [H]

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQQAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLL
RVKEHYTRLLPDYPRFEIAESFFNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSGTLPFLLPIHQTDDGELFIDTCL
TTTAEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRG
MVMLVFTLPGFDRVFKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMELLLQEAAEK
ITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEIC
YMTEVNFRDIPPPRYPEDELASEPWYSVSPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDV
YAYRRRQRFSVRYGEMLF

Sequences:

>Translated_578_residues
MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQQAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLL
RVKEHYTRLLPDYPRFEIAESFFNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSGTLPFLLPIHQTDDGELFIDTCL
TTTAEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRG
MVMLVFTLPGFDRVFKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMELLLQEAAEK
ITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEIC
YMTEVNFRDIPPPRYPEDELASEPWYSVSPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDV
YAYRRRQRFSVRYGEMLF
>Mature_577_residues
PRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQQAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLR
VKEHYTRLLPDYPRFEIAESFFNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDLP
LRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSGTLPFLLPIHQTDDGELFIDTCLT
TTAEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGM
VMLVFTLPGFDRVFKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMELLLQEAAEKI
TDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEICY
MTEVNFRDIPPPRYPEDELASEPWYSVSPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVY
AYRRRQRFSVRYGEMLF

Specific function: Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source

COG id: COG4579

COG function: function code T; Isocitrate dehydrogenase kinase/phosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AceK family [H]

Homologues:

Organism=Escherichia coli, GI1790446, Length=578, Percent_Identity=99.8269896193772, Blast_Score=1198, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010452
- ProDom:   PD043552 [H]

Pfam domain/function: PF06315 AceK [H]

EC number: =2.7.11.5 [H]

Molecular weight: Translated: 67671; Mature: 67540

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQQAMKNRIHLYDHHVGL
CCCCHHHHHHHHHHHHCCHHCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHH
VVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESFFNSVYCRLFDHRSLTPER
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
LFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDLPLRLRWQNKSRDIHYIIRHL
EEEECCCCHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHH
TETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSGTLPFLLPIHQTDDGELFIDTCL
HHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHEEECCCCCCCEEEEEEECCCCCEEEHHHH
TTTAEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYRE
HHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHCCCCCHHHHHHHHCCHHHCCHHHHHH
YLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRVFKVIKDKFAPQKEMSAAHVRACYQLV
HHHHHHCCCHHHHCCCCCCCEEEHEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
KEHDRVGRMADTQEFENFVLEKRHISPALMELLLQEAAEKITDLGEQIVIRHLYIERRMV
HHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEIC
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEECCCEE
YMTEVNFRDIPPPRYPEDELASEPWYSVSPGDVFPEEFRHWLCADPRIGPLFEEMHADLF
EEEECCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHH
RADYWRALQNRIREGHVEDVYAYRRRQRFSVRYGEMLF
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHCCCC
>Mature Secondary Structure 
PRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQQAMKNRIHLYDHHVGL
CCCHHHHHHHHHHHHCCHHCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHH
VVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESFFNSVYCRLFDHRSLTPER
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
LFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDLPLRLRWQNKSRDIHYIIRHL
EEEECCCCHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHH
TETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSGTLPFLLPIHQTDDGELFIDTCL
HHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHEEECCCCCCCEEEEEEECCCCCEEEHHHH
TTTAEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYRE
HHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHCCCCCHHHHHHHHCCHHHCCHHHHHH
YLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRVFKVIKDKFAPQKEMSAAHVRACYQLV
HHHHHHCCCHHHHCCCCCCCEEEHEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
KEHDRVGRMADTQEFENFVLEKRHISPALMELLLQEAAEKITDLGEQIVIRHLYIERRMV
HHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEIC
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEECCCEE
YMTEVNFRDIPPPRYPEDELASEPWYSVSPGDVFPEEFRHWLCADPRIGPLFEEMHADLF
EEEECCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHH
RADYWRALQNRIREGHVEDVYAYRRRQRFSVRYGEMLF
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]