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The map label for this gene is nfi [H]

Identifier: 157163467

GI number: 157163467

Start: 4228372

End: 4229043

Strand: Direct

Name: nfi [H]

Synonym: EcHS_A4232

Alternate gene names: 157163467

Gene position: 4228372-4229043 (Clockwise)

Preceding gene: 157163466

Following gene: 157163468

Centisome position: 91.06

GC content: 58.78

Gene sequence:

>672_bases
ATGGATCTCGCGTCATTACGCGCTCAACAAATCGAACTGGCTTCTTCTGTGATCCGCGAGGATCGACTCGATAAAGATCC
ACCGGATCTGATCGCCGGAGCCGATGTCGGGTTTGAGCAGGGCGGAGAAGTGACGCGAGCGGCGATGGTGCTGCTGAAAT
ATCCCTCGCTTGAGCTGGTGGAGTATAAAGTTGCCCGCATCGCCACCACCATGCCTTACATTCCAGGTTTTCTTTCCTTC
CGCGAATATCCTGCGCTGCTGGCAGCGTGGGAGATGCTGTCGCAAAAGCCGGATTTAGTGTTTGTCGATGGTCATGGGAT
CTCGCATCCTCGCCGTCTTGGCGTCGCCAGCCATTTTGGTTTGATGGTGGATGTGCCAACGATCGGCGTGGCGAAAAAAC
GGCTCTGCGGTAAATTCGAACCGCTCTCCAGCGAACCGGGCGCGCTGGCCCCACTGATGGATAAAGGCGAGCAGCTGGCC
TGGGTCTGGCGCAGCAAAGCGCGCTGTAACCCGTTGTTTATCGCTACCGGCCATCGGGTCAGCGTGGACAGCGCGCTGGC
GTGGGTACAACGCTGCATGAAAGGCTATCGTCTGCCGGAGCCAACGCGCTGGGCGGACGCGGTGGCCTCGGAACGTCCGG
CGTTCGTGCGCTATACAGCAAATCAGCCCTAA

Upstream 100 bases:

>100_bases
TGGTCACGGCATTCATCAGGATGTGCCGCCAGAACATGCTGGCGTGTTCGTGGAGGCAGTGCATCGACTGTCTGAACAGT
ATCACCGCTAAGGAGTGATT

Downstream 100 bases:

>100_bases
TTCAGGTAAACTGCGGATAATTTCCGTATTTGAGAACTCAACATGTTACAAAACCCAATTCATCTGCGTCTGGAGCGCCT
GGAAAGCTGGCAGCATGTCA

Product: endonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEVTRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSF
REYPALLAAWEMLSQKPDLVFVDGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRYTANQP

Sequences:

>Translated_223_residues
MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEVTRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSF
REYPALLAAWEMLSQKPDLVFVDGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRYTANQP
>Mature_223_residues
MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEVTRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSF
REYPALLAAWEMLSQKPDLVFVDGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRYTANQP

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair [H]

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family [H]

Homologues:

Organism=Homo sapiens, GI31542775, Length=199, Percent_Identity=36.6834170854271, Blast_Score=121, Evalue=5e-28,
Organism=Homo sapiens, GI257467548, Length=194, Percent_Identity=29.3814432989691, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI257467550, Length=126, Percent_Identity=35.7142857142857, Blast_Score=75, Evalue=5e-14,
Organism=Escherichia coli, GI87082357, Length=223, Percent_Identity=99.5515695067265, Blast_Score=455, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007581 [H]

Pfam domain/function: PF04493 Endonuclease_5 [H]

EC number: =3.1.21.7 [H]

Molecular weight: Translated: 24691; Mature: 24691

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEVTRAAMVLLKYPSLELV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCHHHH
EYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFVDGHGISHPRRLGVASHFG
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHCHHHHCC
LMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLAWVWRSKARCNPLFIATGHRV
EEEECCCCCHHHHHHCCCCCCCCCCCCCCCHHHHCCCEEEEEECCCCCCCEEEEECCCEE
SVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRYTANQP
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEECCCCC
>Mature Secondary Structure
MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEVTRAAMVLLKYPSLELV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCHHHH
EYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFVDGHGISHPRRLGVASHFG
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHCHHHHCC
LMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLAWVWRSKARCNPLFIATGHRV
EEEECCCCCHHHHHHCCCCCCCCCCCCCCCHHHHCCCEEEEEECCCCCCCEEEEECCCEE
SVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRYTANQP
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA