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The map label for this gene is mdtL [H]

Identifier: 157163191

GI number: 157163191

Start: 3918787

End: 3919962

Strand: Direct

Name: mdtL [H]

Synonym: EcHS_A3924

Alternate gene names: 157163191

Gene position: 3918787-3919962 (Clockwise)

Preceding gene: 157163190

Following gene: 157163192

Centisome position: 84.39

GC content: 52.55

Gene sequence:

>1176_bases
ATGTCCCGCTTTTTAATTTGTAGTTTTGCCCTGGTTTTACTTTATCCCGCCGGGATTGATATGTACCTCGTTGGTTTACC
GCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTGCATATTGCGTTCTCCGTATATCTGGCGGGGATGGCAGCTG
CGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATT
GCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTG
TTGTTACGTAGTGGCGTTCGCCATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACG
GTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTC
TGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGTCCCCCGC
TGCTTCGGACAAACCACGAGAAAATAGCGAGTCGCTGCTTAATCGGTTTTTCCTCAGCCGTGTTGTTATCACCACCCTCA
GCGTTTCGGTGATCCTCACTTTCGTCAATACATCGCCGGTATTGCTGATGGAAATCATGGGTTTTGAGCGCGGAGAATAC
GCCACCATTATGGCGTTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCC
ACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTTGCAGCGGGGATCACCCTTGCCGTTTCACCTTCCCATGCGGTTT
CTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGCGTAGCGATGAGTCAGGCGTTAGGACCATTT
TCATTACGCGCGGGCGTAGCCAGCTCGACCTTAGGTATTGCGCAGGTTTGCGGTTCGTCACTGTGGATTTGGCTGGCAGC
GGTGGTTGGTATCGGCGCATGGAATATGCTGATCGGGATTCTGATTGCCTGTAGCATAGTGAGCCTGTTGCTGATTATGT
TCGTCGCGCCTGGACGCCCCGTTGCCGCTCATGAAGAAATCCATCACCACGCTTGA

Upstream 100 bases:

>100_bases
CTTGCCTGTCAGGAAATCTCTTTTGTCCAAATGGCAACTCGCCTGATTCTCCTTCACCACGTATGCTTTGCGTCACCTTA
CTATCAGGACGCTTTAGCCC

Downstream 100 bases:

>100_bases
TCTCAATCTGCTGCTCTGTCTGCAACTGCTGATGCAGGAGCGCAGCGTGACCAAAGCGGCGAAGCGGATGAACGTGACCC
CTTCAGCGGTGAGTAAGTCG

Product: multidrug efflux system protein MdtL

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAFSVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFII
ASVFCSLAETSTLFLAGRFLQGLGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVILTFVNTSPVLLMEIMGFERGEY
ATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQVLFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPF
SLRAGVASSTLGIAQVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA

Sequences:

>Translated_391_residues
MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAFSVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFII
ASVFCSLAETSTLFLAGRFLQGLGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVILTFVNTSPVLLMEIMGFERGEY
ATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQVLFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPF
SLRAGVASSTLGIAQVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA
>Mature_390_residues
SRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAFSVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIA
SVFCSLAETSTLFLAGRFLQGLGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLFW
AMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVILTFVNTSPVLLMEIMGFERGEYA
TIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQVLFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFS
LRAGVASSTLGIAQVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA

Specific function: Confers resistance to chloramphenicol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. MdtL (TC 2.A.1.2.22) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790146, Length=391, Percent_Identity=99.7442455242967, Blast_Score=772, Evalue=0.0,
Organism=Escherichia coli, GI1788509, Length=226, Percent_Identity=30.9734513274336, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI48994889, Length=242, Percent_Identity=29.3388429752066, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790794, Length=225, Percent_Identity=28.4444444444444, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082312, Length=343, Percent_Identity=26.8221574344023, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787065, Length=233, Percent_Identity=23.6051502145923, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI87081738, Length=148, Percent_Identity=31.0810810810811, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41506; Mature: 41375

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAFSVYLAGMAAAMLFAGK
CCCHHHHHHHHHHHHHCCCCEEEEECHHHHHCCCCCHHHEEHHHHHHHHHHHHHHHHHHH
VADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQGLGAGCCYVVAFAILRDTL
HHCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLFWAMAMMGIAVLMLSLFILKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
TRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVILTFVNTSPVLLMEIMGFERGEY
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCH
ATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQVLFLAAGITLAVSPSHAVSLFGITL
HHHEEECCCCEEEEEECCCEEEECCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHH
ICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIAQVCGSSLWIWLAAVVGIGAWNMLIGI
HHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHH
LIACSIVSLLLIMFVAPGRPVAAHEEIHHHA
HHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC
>Mature Secondary Structure 
SRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAFSVYLAGMAAAMLFAGK
CCHHHHHHHHHHHHHCCCCEEEEECHHHHHCCCCCHHHEEHHHHHHHHHHHHHHHHHHH
VADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQGLGAGCCYVVAFAILRDTL
HHCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLFWAMAMMGIAVLMLSLFILKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
TRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVILTFVNTSPVLLMEIMGFERGEY
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCH
ATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQVLFLAAGITLAVSPSHAVSLFGITL
HHHEEECCCCEEEEEECCCEEEECCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHH
ICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIAQVCGSSLWIWLAAVVGIGAWNMLIGI
HHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHH
LIACSIVSLLLIMFVAPGRPVAAHEEIHHHA
HHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA