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The map label for this gene is coaBC

Identifier: 157163120

GI number: 157163120

Start: 3842312

End: 3843532

Strand: Direct

Name: coaBC

Synonym: EcHS_A3848

Alternate gene names: 157163120

Gene position: 3842312-3843532 (Clockwise)

Preceding gene: 157163115

Following gene: 157163121

Centisome position: 82.75

GC content: 53.32

Gene sequence:

>1221_bases
ATGAGCCTGGCCGGTAAAAAAATCGTTCTCGGCGTTAGCGGCGGTATTGCTGCCTATAAAACCCCTGAACTGGTGCGTCG
TTTGCGCGATCGCGGGGCCGACGTCCGCGTAGCCATGACCGAAGCGGCAAAAGCCTTTATCACCCCACTTAGCTTGCAGG
CGGTTTCTGGTTATCCCGTTTCCGACAGTTTGCTGGACCCGGCAGCCGAAGCCGCTATGGGCCATATTGAGCTGGGTAAA
TGGGCTGATTTAGTGATTCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGT
ATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCA
CGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGAT
ATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTGGCGCATTTTTCGCCCGTCAACGACCTGAA
ACATCTGAACATTATGATTACCGCCGGTCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTAATCACAGCTCCG
GCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTCCAGTTTCACTA
CCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTCTGTACAGCA
GCAAAATATTTTTATCGGTTGCGCCGCCGTGGCGGATTATCGCGCAGCTACCGTGGCCCCAGAAAAAATCAAAAAGCAGG
CCACGCAGGGTGATGAATTAACAATAAAAATGGTTAAAAACCCCGATATCGTCGCAGGCGTTGCCGCACTAAAAGACCAT
CGACCCTACGTCGTTGGATTTGCCGCCGAAACAAATAATGTGGAAGAATACGCCCGGCAAAAACGTATCCGTAAAAACCT
TGATCTGATCTGCGCGAACGATGTTTCCCAGCCAACTCAAGGATTTAACAGCGACAACAACGCATTACACCTTTTCTGGC
AGGACGGAGATAAAGTCTTACCGCTTGAGCGCAAAGAGCTCCTTGGCCAATTATTACTCGACGAGATCGTGACCCGTTAT
GATGAAAAAAATCGACGTTAA

Upstream 100 bases:

>100_bases
GGAAAGCGCCTCGCAAAGTGAATCTCAGGTGAAGGCACGACAACAAAAGTATTGTGATAAAATCGCCAACTTCTGGTGTC
ACCCTACAGGAAAAATCAAC

Downstream 100 bases:

>100_bases
GATTCTGGACCCGCGCGTTGGGAAGGAATTTCCGCTCCCGACTTATGCCACCTCTGGCTCTGCCGGACTTGACCTGCGTG
CCTGTCTCGACGACGCCGTA

Product: bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate synthase

Products: NA

Alternate protein names: DNA/pantothenate metabolism flavoprotein; Phosphopantothenoylcysteine decarboxylase; PPCDC; CoaC; Phosphopantothenate--cysteine ligase; CoaB; PPC synthetase; PPCS; Phosphopantothenoylcysteine synthase

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAKAFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGK
WADLVILAPATADLIARVAAGMANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGFAIAAAAARRGANVTLVSGPVSL
PTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAAVADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDH
RPYVVGFAAETNNVEEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLLLDEIVTRY
DEKNRR

Sequences:

>Translated_406_residues
MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAKAFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGK
WADLVILAPATADLIARVAAGMANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGFAIAAAAARRGANVTLVSGPVSL
PTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAAVADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDH
RPYVVGFAAETNNVEEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLLLDEIVTRY
DEKNRR
>Mature_405_residues
SLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAKAFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKW
ADLVILAPATADLIARVAAGMANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGDI
GPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGFAIAAAAARRGANVTLVSGPVSLP
TPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAAVADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHR
PYVVGFAAETNNVEEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLLLDEIVTRYD
EKNRR

Specific function: Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine

COG id: COG0452

COG function: function code H; Phosphopantothenoylcysteine synthetase/decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PPC synthetase family

Homologues:

Organism=Homo sapiens, GI71725351, Length=175, Percent_Identity=32.5714285714286, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI87082301, Length=406, Percent_Identity=100, Blast_Score=829, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17560194, Length=191, Percent_Identity=30.8900523560209, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322762, Length=185, Percent_Identity=27.027027027027, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24657262, Length=191, Percent_Identity=30.3664921465969, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COABC_ECOL6 (P0ABQ1)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_756325.2
- ProteinModelPortal:   P0ABQ1
- SMR:   P0ABQ1
- EnsemblBacteria:   EBESCT00000044598
- GeneID:   1038234
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c4463
- GeneTree:   EBGT00050000011616
- HOGENOM:   HBG523890
- OMA:   VTSGPTH
- ProtClustDB:   PRK05579
- BRENDA:   4.1.1.36
- BRENDA:   6.3.2.5
- InterPro:   IPR005252
- InterPro:   IPR007085
- InterPro:   IPR003382
- Gene3D:   G3DSA:3.40.50.10300
- Gene3D:   G3DSA:3.40.50.1950
- TIGRFAMs:   TIGR00521

Pfam domain/function: PF04127 DFP; PF02441 Flavoprotein; SSF102645 DNA/pantothenate-metab_flavo_C; SSF52507 Flavoprotein

EC number: =4.1.1.36; =6.3.2.5

Molecular weight: Translated: 43439; Mature: 43307

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: ACT_SITE 158-158 BINDING 125-125 BINDING 279-279 BINDING 289-289 BINDING 341-341 BINDING 345-345

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAKAFITPLSLQAVSGYPV
CCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHCCCEEEEECCCCC
SDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGMANDLVSTICLATPAPVAV
CHHHHCHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGDIGPGRMLDPLTIVDMAVAHF
HHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
SPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGFAIAAAAARRGANVTLVSGPVSL
CCCCCCCEEEEEEEECCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEECCCCC
PTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAAVADYRAATVAPEKIKKQATQGDEL
CCCHHHHHHHHHHHHHHHHHHCCCEECCEEEEEEEHHHCCHHHHCCHHHHHHHCCCCCCE
TIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNVEEYARQKRIRKNLDLICANDVSQPTQ
EEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCCCC
GFNSDNNALHLFWQDGDKVLPLERKELLGQLLLDEIVTRYDEKNRR
CCCCCCCEEEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAKAFITPLSLQAVSGYPV
CCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHCCCEEEEECCCCC
SDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGMANDLVSTICLATPAPVAV
CHHHHCHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGDIGPGRMLDPLTIVDMAVAHF
HHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
SPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGFAIAAAAARRGANVTLVSGPVSL
CCCCCCCEEEEEEEECCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEECCCCC
PTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAAVADYRAATVAPEKIKKQATQGDEL
CCCHHHHHHHHHHHHHHHHHHCCCEECCEEEEEEEHHHCCHHHHCCHHHHHHHCCCCCCE
TIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNVEEYARQKRIRKNLDLICANDVSQPTQ
EEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCCCC
GFNSDNNALHLFWQDGDKVLPLERKELLGQLLLDEIVTRYDEKNRR
CCCCCCCEEEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157