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The map label for this gene is sgbE

Identifier: 157163061

GI number: 157163061

Start: 3779389

End: 3780084

Strand: Direct

Name: sgbE

Synonym: EcHS_A3787

Alternate gene names: 157163061

Gene position: 3779389-3780084 (Clockwise)

Preceding gene: 157163060

Following gene: 157163063

Centisome position: 81.39

GC content: 57.33

Gene sequence:

>696_bases
ATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGG
CAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGTACGACGTGATGACCGCCG
ACGATATGGTGGTGGTTGAGATAGCCAGCGGTAAGGTGGTGGAAGGCAGCAAAAAACCCTCTTCCGATACACCAACGCAT
CTGGCGCTCTACCGTCGCTATGCCGAAATTGGCGGTATTGTGCATACCCACTCGCGCCACGCCACCATCTGGTCACAGGC
CGGGCTGGATCTCCCCGCCTGGGGCACCACCCACGCCGATTATTTTTACGGTGCCATCCCCTGCACGCGACAGATGACCG
CAGAGGAGATTAACGGCGAATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCG
GCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGC
CGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAAC
TGCTGGATAAGCACTACCTGCGTAAGCATGGGGCCAATGCCTATTACGGGCAGTAA

Upstream 100 bases:

>100_bases
TCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGT
ATGCAGGAGGCTGGATTTAT

Downstream 100 bases:

>100_bases
TCCCTCACGCCGGGGCTTCATCGCCCCGGCACTACGAATTGATATGTTCCTTGCTGTAACGCCGCTTCCACGCTGCTGGC
ATTAAACCAGTATGTTTCTG

Product: L-ribulose-5-phosphate 4-epimerase

Products: NA

Alternate protein names: Phosphoribulose isomerase

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPSGVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTH
LALYRRYAEIGGIVHTHSRHATIWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKHYLRKHGANAYYGQ

Sequences:

>Translated_231_residues
MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPSGVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTH
LALYRRYAEIGGIVHTHSRHATIWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKHYLRKHGANAYYGQ
>Mature_231_residues
MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPSGVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTH
LALYRRYAEIGGIVHTHSRHATIWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKHYLRKHGANAYYGQ

Specific function: Catalyzes the isomerization of L-ribulose 5-phosphate to D-xylulose 5-phosphate. May be involved in the utilization of 2,3- diketo-L-gulonate

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family. AraD/FucA subfamily

Homologues:

Organism=Escherichia coli, GI1790008, Length=231, Percent_Identity=100, Blast_Score=483, Evalue=1e-138,
Organism=Escherichia coli, GI1786247, Length=231, Percent_Identity=76.1904761904762, Blast_Score=370, Evalue=1e-104,
Organism=Escherichia coli, GI1790642, Length=230, Percent_Identity=61.304347826087, Blast_Score=293, Evalue=9e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SGBE_ECOLI (P37680)

Other databases:

- EMBL:   U00039
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   S47804
- RefSeq:   AP_004209.1
- RefSeq:   NP_418040.1
- ProteinModelPortal:   P37680
- SMR:   P37680
- STRING:   P37680
- EnsemblBacteria:   EBESCT00000002358
- EnsemblBacteria:   EBESCT00000002359
- EnsemblBacteria:   EBESCT00000014278
- GeneID:   948099
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3555
- KEGG:   eco:b3583
- EchoBASE:   EB2195
- EcoGene:   EG12287
- eggNOG:   COG0235
- GeneTree:   EBGT00050000009319
- HOGENOM:   HBG541069
- OMA:   PCTREMY
- ProtClustDB:   PRK12347
- BioCyc:   EcoCyc:EG12287-MONOMER
- BioCyc:   MetaCyc:EG12287-MONOMER
- Genevestigator:   P37680
- InterPro:   IPR001303
- InterPro:   IPR004661
- Gene3D:   G3DSA:3.40.225.10
- TIGRFAMs:   TIGR00760

Pfam domain/function: PF00596 Aldolase_II; SSF53639 Aldolase_II_N

EC number: =5.1.3.4

Molecular weight: Translated: 25561; Mature: 25561

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPSGVEYDVMTADDMVVVE
CCHHHHHHHHHHHHCCCCEEEEEEECCCEEECCCCCCEEEECCCCCEEEEEECCCEEEEE
IASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHATIWSQAGLDLPAWGTTHAD
ECCCEEEECCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCEEEHHHCCCCCCCCCCCCCC
YFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSPAQIPAVLVHSHGPFAWGKNA
CEEECCCCCHHHHHHHCCCEEEECCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCH
ADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKHYLRKHGANAYYGQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPSGVEYDVMTADDMVVVE
CCHHHHHHHHHHHHCCCCEEEEEEECCCEEECCCCCCEEEECCCCCEEEEEECCCEEEEE
IASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHATIWSQAGLDLPAWGTTHAD
ECCCEEEECCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCEEEHHHCCCCCCCCCCCCCC
YFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSPAQIPAVLVHSHGPFAWGKNA
CEEECCCCCHHHHHHHCCCEEEECCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCH
ADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKHYLRKHGANAYYGQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 11741871