Click here to switch to the map view.

The map label for this gene is rtcR [H]

Identifier: 157162899

GI number: 157162899

Start: 3602543

End: 3604135

Strand: Direct

Name: rtcR [H]

Synonym: EcHS_A3619

Alternate gene names: 157162899

Gene position: 3602543-3604135 (Clockwise)

Preceding gene: 157162896

Following gene: 157162903

Centisome position: 77.58

GC content: 55.18

Gene sequence:

>1593_bases
ATGCGTAAAACAGTGGCTTTTGGCTTTGTCGGTACCGTGCTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCGAAATG
GCTTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTATTGCATGACGCCCGCTCGCTAT
TTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGCATAACCCG
TGGGATTTCGAAGAGGTTTACGCTTGCCTGCATGATTTCGCCCGTGGTTACGAGTTTCAGCCAGAAAAAGAAGACTATTT
AATTCACATTACCACCGGCACCCACGTCGCGCAGATTTGCTGGTTTCTGCTGGCGGAAGCGCGTTACCTGCCCGCCCGGC
TGATACAATCTTCACCTCCGCGCAAAAAAGAACAGCCGCGCGGCGCAGGTGAAGTGACGATTATCGATCTCGATTTAAGC
CGTTATAACGCCATCGCCAGCCGCTTTGCCGAGGAACGCCAGCAAACGCTTGATTTTCTTAAGTCCGGCATTGCCACGCG
TAACCCCCACTTCAACCGCATGATTGAGCAGATCGAAAAAGTGGCGATCAAATCCCGCGCGCCGATTCTGCTTAACGGTC
CAACCGGCGCGGGCAAGTCATTTCTGGCGCGACGCATCTTCGAGTTAAAACAGGCGCGGCATCAGTTTAGCGGCGCGTTT
GTGGAAGTGAACTGCGCCACCCTGCGCGGCGATACCGCCATGTCGACGCTGTTTGGTCATGTAAAAGGCGCGTTTACCGG
GGCGCGGGAATCTCGTGAAGGTTTATTACGCAGCGCCAACGGCGGAATGTTGTTTCTTGATGAGATTGGCGAACTGGGCG
CAGACGAACAAGCAATGCTGCTAAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCAGGTGAGCAGT
GATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAGCTGGTTGCCGAAGGCAAATTCCGCGAAGATCTGTACGC
GCGGATCAATCTCTGGACCTTCACCCTGCCGGGGCTACGCCAGCGCCAGGAAGATATTGAACCTAACCTGGATTATGAAG
TGGAGCGCCACGCCTCACTCACCGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGTGCCTGGCTGGCCTTTGCAACA
TCTCCTCAGGCGACATGGCGCGGTAACTTTCGCGAGCTTTCTGCCAGCGTCACGCGGATGGCAACCTTTGCCACTAGCGG
ACGCATCACTCTGGACGTGGTTGAAGATGAGATAAACCGTCTGCGCTATAACTGGCAGGAGAGCCGTCCCTCCGCGCTTA
CGCAACTGCTGGGCGCAGAGGCAGAAAACATCGATCTCTTCGACCGTATGCAACTGGAACACGTTATCGCTATCTGCCGC
CAGGCAAAGTCGCTTTCTGCTGCCGGACGACAGCTTTTTGATGTTTCGCGCCAGGGCAAAGCCAGCGTCAATGACGCGGA
TCGGCTACGCAAATACCTGGCGCGTTTTGGTCTGACCTGGGAAGCATTGCAGGATCAGCACAGCTCCAGTTGA

Upstream 100 bases:

>100_bases
TTGTTTTTAAATAGGTTAAATCTTTACGTCCGTAAACGGAGATTTTCCACAAAACCAATTCACCGATAATGAAATATCGT
CTTTTATAAGGATATCTAAG

Downstream 100 bases:

>100_bases
ATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCTGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCT
GCCCATATTGACCATCGCGT

Product: sigma-54 dependent transcriptional regulator RtcR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MRKTVAFGFVGTVLDYAGRGSQRWSKWLPTLCLCQQESLVIDRLELLHDARSLFETLKRDIASVSPETEVVSVEIELHNP
WDFEEVYACLHDFARGYEFQPEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLDLS
RYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSGAF
VEVNCATLRGDTAMSTLFGHVKGAFTGARESREGLLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSS
DFQLIAGTVRDLRQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTEARRAWLAFAT
SPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQESRPSALTQLLGAEAENIDLFDRMQLEHVIAICR
QAKSLSAAGRQLFDVSRQGKASVNDADRLRKYLARFGLTWEALQDQHSSS

Sequences:

>Translated_530_residues
MRKTVAFGFVGTVLDYAGRGSQRWSKWLPTLCLCQQESLVIDRLELLHDARSLFETLKRDIASVSPETEVVSVEIELHNP
WDFEEVYACLHDFARGYEFQPEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLDLS
RYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSGAF
VEVNCATLRGDTAMSTLFGHVKGAFTGARESREGLLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSS
DFQLIAGTVRDLRQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTEARRAWLAFAT
SPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQESRPSALTQLLGAEAENIDLFDRMQLEHVIAICR
QAKSLSAAGRQLFDVSRQGKASVNDADRLRKYLARFGLTWEALQDQHSSS
>Mature_530_residues
MRKTVAFGFVGTVLDYAGRGSQRWSKWLPTLCLCQQESLVIDRLELLHDARSLFETLKRDIASVSPETEVVSVEIELHNP
WDFEEVYACLHDFARGYEFQPEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLDLS
RYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSGAF
VEVNCATLRGDTAMSTLFGHVKGAFTGARESREGLLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSS
DFQLIAGTVRDLRQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTEARRAWLAFAT
SPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQESRPSALTQLLGAEAENIDLFDRMQLEHVIAICR
QAKSLSAAGRQLFDVSRQGKASVNDADRLRKYLARFGLTWEALQDQHSSS

Specific function: Transcriptional repressor of the rtcAB genes. Interacts with sigma-54 [H]

COG id: COG4650

COG function: function code KT; Sigma54-dependent transcription regulator containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789828, Length=532, Percent_Identity=98.6842105263158, Blast_Score=1075, Evalue=0.0,
Organism=Escherichia coli, GI1788905, Length=275, Percent_Identity=36, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI1788550, Length=248, Percent_Identity=38.7096774193548, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1790437, Length=256, Percent_Identity=39.453125, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1789233, Length=264, Percent_Identity=35.6060606060606, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI87082117, Length=302, Percent_Identity=33.112582781457, Blast_Score=145, Evalue=7e-36,
Organism=Escherichia coli, GI1789087, Length=254, Percent_Identity=36.2204724409449, Blast_Score=142, Evalue=7e-35,
Organism=Escherichia coli, GI1787583, Length=244, Percent_Identity=36.0655737704918, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI87082152, Length=247, Percent_Identity=36.4372469635627, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1790299, Length=241, Percent_Identity=35.6846473029046, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1786524, Length=239, Percent_Identity=35.9832635983264, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI87081872, Length=254, Percent_Identity=33.0708661417323, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI87081858, Length=242, Percent_Identity=29.7520661157025, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR002078
- InterPro:   IPR009715
- InterPro:   IPR017183 [H]

Pfam domain/function: PF06956 RtcR; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 60093; Mature: 60093

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTVAFGFVGTVLDYAGRGSQRWSKWLPTLCLCQQESLVIDRLELLHDARSLFETLKRD
CCCEEHHHHHHHHHHHCCCCHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
IASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQPEKEDYLIHITTGTHVAQIC
HHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHH
WFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLDLSRYNAIASRFAEERQQTLDFL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
KSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSGAF
HHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCE
VEVNCATLRGDTAMSTLFGHVKGAFTGARESREGLLRSANGGMLFLDEIGELGADEQAML
EEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEHHHCCCCCHHHHH
LKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDLRQLVAEGKFREDLYARINLWTFTLPGLR
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEEEECCCHH
QRQEDIEPNLDYEVERHASLTGDSVRFNTEARRAWLAFATSPQATWRGNFRELSASVTRM
HHHHCCCCCCCCCHHHHCCCCCCCEEECHHHHHEEEEEECCCCCHHCCCHHHHHHHHHHH
ATFATSGRITLDVVEDEINRLRYNWQESRPSALTQLLGAEAENIDLFDRMQLEHVIAICR
HHHCCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
QAKSLSAAGRQLFDVSRQGKASVNDADRLRKYLARFGLTWEALQDQHSSS
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCC
>Mature Secondary Structure
MRKTVAFGFVGTVLDYAGRGSQRWSKWLPTLCLCQQESLVIDRLELLHDARSLFETLKRD
CCCEEHHHHHHHHHHHCCCCHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
IASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQPEKEDYLIHITTGTHVAQIC
HHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHH
WFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLDLSRYNAIASRFAEERQQTLDFL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
KSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSGAF
HHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCE
VEVNCATLRGDTAMSTLFGHVKGAFTGARESREGLLRSANGGMLFLDEIGELGADEQAML
EEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEHHHCCCCCHHHHH
LKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDLRQLVAEGKFREDLYARINLWTFTLPGLR
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEEEECCCHH
QRQEDIEPNLDYEVERHASLTGDSVRFNTEARRAWLAFATSPQATWRGNFRELSASVTRM
HHHHCCCCCCCCCHHHHCCCCCCCEEECHHHHHEEEEEECCCCCHHCCCHHHHHHHHHHH
ATFATSGRITLDVVEDEINRLRYNWQESRPSALTQLLGAEAENIDLFDRMQLEHVIAICR
HHHCCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
QAKSLSAAGRQLFDVSRQGKASVNDADRLRKYLARFGLTWEALQDQHSSS
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 3045764; 7984428; 7920643; 9738023 [H]