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The map label for this gene is gntT

Identifier: 157162893

GI number: 157162893

Start: 3590900

End: 3592216

Strand: Direct

Name: gntT

Synonym: EcHS_A3613

Alternate gene names: 157162893

Gene position: 3590900-3592216 (Clockwise)

Preceding gene: 157162892

Following gene: 157162896

Centisome position: 77.33

GC content: 53.3

Gene sequence:

>1317_bases
ATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGC
TCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTG
TCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGC
GCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTAC
CGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATA
TTCCACTGCTGTATGTTGGTGTACCAATGGCAGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCG
ACTGCGATTGCCACCATTTTCAATGCCGATATGGGTAAAACCCTGCTGTACGGTACTATTCTGGCAATCCCGACCGTGAT
TCTCGCCGGTCCGGTTTACGCTCGCGTGTTGAAAGGTATCGATAAGCCAATTCCGGAAGGTCTGTACAGCGCGAAAACCT
TCAGCGAAGAAGAGATGCCGAGCTTTGGCGTCAGCGTCTGGACCTCTCTGGTGCCGGTAGTACTGATGGCGATGCGTGCG
ATTGCCGAAATGATCCTGCCGAAAGGTCACGCTTTCCTGCCGGTAGCGGAGTTCCTCGGTGACCCGGTAATGGCAACGCT
GATTGCCGTGCTGATTGCGATGTTCACCTTTGGTCTAAACCGTGGTCGTTCAATGGATCAGATTAACGACACGCTGGTTT
CTTCCATCAAAATCATTGCGATGATGCTGTTGATCATCGGTGGTGGCGGTGCGTTCAAGCAGGTGCTGGTAGACAGCGGC
GTGGACAAATACATTGCTTCCATGATGCACGAAACCAACATTTCTCCGCTGCTGATGGCCTGGTCGATTGCTGCCGTACT
GCGTATCGCGCTGGGTTCCGCAACCGTTGCGGCAATCACTGCGGGTGGTATCGCGGCACCGCTGATTGCAACGACGGGTG
TTAGCCCGGAGCTGATGGTTATTGCGGTTGGTTCCGGTAGTGTGATTTTCTCTCATGTGAACGATCCGGGCTTCTGGCTG
TTCAAAGAGTACTTTAACCTGACTATCGGCGAGACCATCAAATCCTGGTCGATGCTGGAAACCATTATCTCGGTGTGCGG
TCTGGTAGGCTGTCTGCTGCTGAATATGGTGATTTGA

Upstream 100 bases:

>100_bases
CAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGATAATTAGAGTTTGTCGTCAGAAAATTGACGTTACCCATAACA
AATGAAAGGCCAGGTAAATC

Downstream 100 bases:

>100_bases
TAACACTGACTGCCGGATGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTC
CAAATCCTTCAGCAACTTGT

Product: high-affinity gluconate transporter

Products: Proton [Cytoplasm]; gluconate [Cytoplasm] [C]

Alternate protein names: Gluconate permease; Gnt-I system

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLDKVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGG
AQRIATTLIAKFGKKHIQWAVVLTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEEMPSFGVSVWTSLVPVVLMAMRA
IAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSG
VDKYIASMMHETNISPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDPGFWL
FKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI

Sequences:

>Translated_438_residues
MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLDKVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGG
AQRIATTLIAKFGKKHIQWAVVLTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEEMPSFGVSVWTSLVPVVLMAMRA
IAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSG
VDKYIASMMHETNISPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDPGFWL
FKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI
>Mature_437_residues
PLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLDKVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGA
QRIATTLIAKFGKKHIQWAVVLTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGPT
AIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEEMPSFGVSVWTSLVPVVLMAMRAI
AEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGV
DKYIASMMHETNISPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDPGFWLF
KEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI

Specific function: Part of the gluconate utilization system Gnt-I; high- affinity intake of gluconate

COG id: COG2610

COG function: function code GE; H+/gluconate symporter and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gntP permease family

Homologues:

Organism=Escherichia coli, GI48994935, Length=438, Percent_Identity=100, Blast_Score=857, Evalue=0.0,
Organism=Escherichia coli, GI1790716, Length=438, Percent_Identity=61.8721461187215, Blast_Score=544, Evalue=1e-156,
Organism=Escherichia coli, GI2367370, Length=449, Percent_Identity=49.2204899777283, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1790776, Length=438, Percent_Identity=40.6392694063927, Blast_Score=348, Evalue=3e-97,
Organism=Escherichia coli, GI1788707, Length=440, Percent_Identity=36.8181818181818, Blast_Score=274, Evalue=1e-74,
Organism=Escherichia coli, GI48994939, Length=434, Percent_Identity=36.405529953917, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1789097, Length=442, Percent_Identity=34.6153846153846, Blast_Score=227, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GNTT_ECOLI (P39835)

Other databases:

- EMBL:   U18997
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   M32793
- PIR:   JC4988
- RefSeq:   AP_004375.1
- RefSeq:   YP_026217.1
- ProteinModelPortal:   P39835
- STRING:   P39835
- EnsemblBacteria:   EBESCT00000000854
- EnsemblBacteria:   EBESCT00000000855
- EnsemblBacteria:   EBESCT00000000856
- EnsemblBacteria:   EBESCT00000016485
- GeneID:   947924
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5690
- KEGG:   eco:b3415
- EchoBASE:   EB2282
- EcoGene:   EG12380
- eggNOG:   COG2610
- GeneTree:   EBGT00050000009137
- HOGENOM:   HBG649941
- OMA:   MIRFKLN
- ProtClustDB:   PRK14984
- BioCyc:   EcoCyc:GNTT-MONOMER
- Genevestigator:   P39835
- InterPro:   IPR003474
- TIGRFAMs:   TIGR00791

Pfam domain/function: PF02447 GntP_permease

EC number: NA

Molecular weight: Translated: 45967; Mature: 45836

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x17392db0)-; HASH(0x173bbae4)-; HASH(0x1705e014)-; HASH(0x17a4cfa4)-; HASH(0x181e5a94)-; HASH(0x17b7fc44)-; HASH(0x17662b54)-; HASH(0x1417ab70)-; HASH(0x173dc6f4)-; HASH(0x17b9c65c)-; HASH(0x1792ed64)-; HASH(0x181d0f04)-; HASH(0x18173be8)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLDKVIGSIKAGVGGTLGS
CCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHH
LALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVVLTGFTVGFALFYEVGFVL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGPTAIATIFNADMGKTLLYGTI
HHHHHHHHHCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHH
LAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEEMPSFGVSVWTSLVPVVLMAMRA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHH
IAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLNRGRSMDQINDTLVSSIKIIA
HHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
MMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNISPLLMAWSIAAVLRIALGSATVAAIT
HHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
AGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDPGFWLFKEYFNLTIGETIKSWSMLE
HCCHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHH
TIISVCGLVGCLLLNMVI
HHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
PLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLDKVIGSIKAGVGGTLGS
CHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHH
LALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVVLTGFTVGFALFYEVGFVL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGPTAIATIFNADMGKTLLYGTI
HHHHHHHHHCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHH
LAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEEMPSFGVSVWTSLVPVVLMAMRA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHH
IAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLNRGRSMDQINDTLVSSIKIIA
HHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
MMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNISPLLMAWSIAAVLRIALGSATVAAIT
HHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
AGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDPGFWLFKEYFNLTIGETIKSWSMLE
HCCHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHH
TIISVCGLVGCLLLNMVI
HHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; gluconate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + gluconate [Periplasm] = Proton [Cytoplasm] + gluconate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 2845225; 7984428; 3040894; 9045817