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The map label for this gene is dam

Identifier: 157162863

GI number: 157162863

Start: 3559422

End: 3560258

Strand: Reverse

Name: dam

Synonym: EcHS_A3583

Alternate gene names: 157162863

Gene position: 3560258-3559422 (Counterclockwise)

Preceding gene: 157162864

Following gene: 157162862

Centisome position: 76.67

GC content: 49.58

Gene sequence:

>837_bases
ATGAAGAAAAATCGCGCTTTTTTGAAGTGGGCAGGGGGCAAGTATCCCCTGCTTGATGATATTAAACGGCATTTGCCCAA
GGGCGAATGTCTGGTTGAGCCTTTTGTAGGTGCCGGGTCGGTGTTTCTCAACACCGACTTTTCTCGTTATATCCTTGCCG
ATATCAATAGCGACCTGATCAGTCTCTATAACATTGTGAAGATGCGTACTGATGAGTACGTACAGGCCGCTCGCGAGCTG
TTTGTTCCCGAAACAAATTGCGCCGAGGTTTACTATCAGTTCCGCGAAGAGTTCAACAAAAGCCAGGATCCGTTCCGTCG
GGCGGTACTGTTTTTATATTTGAACCGCTACGGTTACAACGGCCTGTGTCGTTACAATCTGCGCGGTGAGTTTAACGTGC
CGTTCGGCCGCTACAAAAAACCCTATTTCCCGGAAGCAGAGTTGTATCACTTCGCTGAAAAAGCGCAGAATGCCTTTTTC
TATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCAGATGATGCATCCGTCGTCTATTGCGATCCGCCTTATGCACCGCT
GTCTGCGACCGCCAACTTTACGGCGTATCACACAAACAGTTTTACGCTTGAACAACAAGCGCATCTGGCGGAGATCGCCG
AAGGTCTGGTTGAGCGCCATATTCCTGTGCTGATCTCCAATCACGATACGATGTTAACGCGTGAGTGGTATCAGCGCGCA
AAATTGCATGTCGTCAAAGTTCGACGCAGTATAAGCAGCAACGGCGGCACACGTAAAAAGGTGGACGAACTGCTGGCTTT
GTACAAACCAGGAGTCGTTTCACCCGCGAAAAAATAA

Upstream 100 bases:

>100_bases
TTATCTCCCGCAATGGTTTATCGTTGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAA
GGTGTAATTAGTTAGTCAGC

Downstream 100 bases:

>100_bases
TTCTCAAGGAGAAGCGGATGAAACAGTATTTGATTGCCCCCTCAATTCTGTCGGCTGATTTTGCCCGCCTGGGTGAAGAT
ACCGCAAAAGCCCTGGCAGC

Product: DNA adenine methylase

Products: NA

Alternate protein names: DNA adenine methyltransferase; Deoxyadenosyl-methyltransferase

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLNTDFSRYILADINSDLISLYNIVKMRTDEYVQAAREL
FVPETNCAEVYYQFREEFNKSQDPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVERHIPVLISNHDTMLTREWYQRA
KLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPAKK

Sequences:

>Translated_278_residues
MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLNTDFSRYILADINSDLISLYNIVKMRTDEYVQAAREL
FVPETNCAEVYYQFREEFNKSQDPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVERHIPVLISNHDTMLTREWYQRA
KLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPAKK
>Mature_278_residues
MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLNTDFSRYILADINSDLISLYNIVKMRTDEYVQAAREL
FVPETNCAEVYYQFREEFNKSQDPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVERHIPVLISNHDTMLTREWYQRA
KLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPAKK

Specific function: Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplica

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family

Homologues:

Organism=Escherichia coli, GI1789789, Length=278, Percent_Identity=100, Blast_Score=580, Evalue=1e-167,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DMA_ECO57 (P0AEE9)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C86003
- PIR:   E91157
- RefSeq:   NP_289926.1
- RefSeq:   NP_312256.1
- ProteinModelPortal:   P0AEE9
- SMR:   P0AEE9
- MINT:   MINT-1230135
- REBASE:   5029
- REBASE:   5592
- EnsemblBacteria:   EBESCT00000023870
- EnsemblBacteria:   EBESCT00000057451
- GeneID:   915915
- GeneID:   958843
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4740
- KEGG:   ecs:ECs4229
- GeneTree:   EBGT00050000011734
- HOGENOM:   HBG586381
- OMA:   KARRTIS
- ProtClustDB:   PRK10904
- BioCyc:   ECOL83334:ECS4229-MONOMER
- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327
- Gene3D:   G3DSA:1.10.1020.10
- PRINTS:   PR00505
- TIGRFAMs:   TIGR00571

Pfam domain/function: PF02086 MethyltransfD12

EC number: =2.1.1.72

Molecular weight: Translated: 32100; Mature: 32100

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS00092 N6_MTASE

Important sites: BINDING 10-10 BINDING 14-14 BINDING 54-54 BINDING 181-181

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLNTDFSRYILADINSDLI
CCCCCEEEEECCCCCCHHHHHHHHCCCCCEEECCCCCCCEEEEECCCCCEEEECCCHHHH
SLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQDPFRRAVLFLYLNRYGYN
HHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
GLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFFYCESYADSMARADDASVVYC
CEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCEEEEE
DPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVERHIPVLISNHDTMLTREWYQRA
CCCCCCCCCCCCEEEEECCCEEHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHH
KLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPAKK
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLNTDFSRYILADINSDLI
CCCCCEEEEECCCCCCHHHHHHHHCCCCCEEECCCCCCCEEEEECCCCCEEEECCCHHHH
SLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQDPFRRAVLFLYLNRYGYN
HHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
GLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFFYCESYADSMARADDASVVYC
CEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCEEEEE
DPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVERHIPVLISNHDTMLTREWYQRA
CCCCCCCCCCCCEEEEECCCEEHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHH
KLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPAKK
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796