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The map label for this gene is gph

Identifier: 157162861

GI number: 157162861

Start: 3557976

End: 3558734

Strand: Reverse

Name: gph

Synonym: EcHS_A3581

Alternate gene names: 157162861

Gene position: 3558734-3557976 (Counterclockwise)

Preceding gene: 157162862

Following gene: 157162860

Centisome position: 76.64

GC content: 52.96

Gene sequence:

>759_bases
ATGAGTAAGTTTGAAGATATTCGCGGCGTCGCTTTTGATCTCGACGGTACGCTGGTGGACAGTGCTCCCGGTCTTGCTGC
TGCGGTAGATATGGCGCTGTATGCGTTGGAGTTACCCGTCGCAGGTGAAGAACGCGTTATTACCTGGATTGGTAACGGCG
CAGATGTTCTGATGGAGCGCGCATTGACCTGGGCGCGTCAGGAACGTGCGACTCTGCGTAAAACAATGGGTAAACCGCCC
GTTGATGACGACATTCCGGCAGAAGAACAGGTACGTATTCTGCGTAAACTGTTCGATCGCTATTATGGCGAGGTTGCCGA
AGAGGGGACGTTTTTGTTCCCGCACGTTGCCGATACGTTGGGCGCGTTGCAGGCTAAAGGCCTGCCGCTAGGCCTGGTCA
CCAACAAACCGACGCCGTTCGTCGCGCCGCTGCTCGAAGCCTTAGATATTGCCAAATACTTTAGCGTGGTTATCGGCGGC
GATGATGTGCAAAACAAAAAACCTCATCCGGACCCGCTGTTACTGGTGGCTGAGCGGATGGGAATTGCCCCACAACAGAT
GCTGTTTGTCGGCGACTCACGCAATGATATTCAGGCGGCAAAAGCGGCAGGTTGCCCATCCGTTGGCTTAACCTACGGAT
ATAACTACGGCGAGGCTATCGATCTCAGCCAGCCTGATGTAATTTATCAGTCTATAAATGACCTTCTGCCCGCATTAGGG
CTTCCGCATAGCGAAAATCAGGAATCGAAAAATGACTAA

Upstream 100 bases:

>100_bases
GCGGGCGCGGATATGTTCGTCGCCGGTTCGGCAATCTTCGACCAGCCAGACTATAAAAAAGTCATTGATGAAATGCGCAG
TGAACTGGCAAAGGTAAGTC

Downstream 100 bases:

>100_bases
GCCCATCGTTTTTAGTGGCGCACAGCCCTCAGGTGAATTGACCATTGGTAACTACATGGGTGCGCTGCGTCAGTGGGTAA
ACATGCAGGATGACTACCAT

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEERVITWIGNGADVLMERALTWARQERATLRKTMGKPP
VDDDIPAEEQVRILRKLFDRYYGEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEAIDLSQPDVIYQSINDLLPALG
LPHSENQESKND

Sequences:

>Translated_252_residues
MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEERVITWIGNGADVLMERALTWARQERATLRKTMGKPP
VDDDIPAEEQVRILRKLFDRYYGEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEAIDLSQPDVIYQSINDLLPALG
LPHSENQESKND
>Mature_251_residues
SKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEERVITWIGNGADVLMERALTWARQERATLRKTMGKPPV
DDDIPAEEQVRILRKLFDRYYGEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGGD
DVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEAIDLSQPDVIYQSINDLLPALGL
PHSENQESKND

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family

Homologues:

Organism=Escherichia coli, GI1789787, Length=252, Percent_Identity=99.2063492063492, Blast_Score=508, Evalue=1e-145,
Organism=Escherichia coli, GI1787576, Length=211, Percent_Identity=23.696682464455, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GPH_ECO57 (P58422)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A86003
- PIR:   C91157
- RefSeq:   NP_289924.1
- RefSeq:   NP_312254.1
- ProteinModelPortal:   P58422
- SMR:   P58422
- EnsemblBacteria:   EBESCT00000027545
- EnsemblBacteria:   EBESCT00000057304
- GeneID:   915918
- GeneID:   958842
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4738
- KEGG:   ecs:ECs4227
- GeneTree:   EBGT00050000009691
- HOGENOM:   HBG742904
- OMA:   YNYGESI
- ProtClustDB:   PRK13222
- BioCyc:   ECOL83334:ECS4227-MONOMER
- HAMAP:   MF_00495
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198
- Gene3D:   G3DSA:3.40.50.1000
- Gene3D:   G3DSA:1.10.150.240
- PRINTS:   PR00413
- TIGRFAMs:   TIGR01549
- TIGRFAMs:   TIGR01509
- TIGRFAMs:   TIGR01449

Pfam domain/function: PF00702 Hydrolase; SSF56784 SSF56784

EC number: =3.1.3.18

Molecular weight: Translated: 27347; Mature: 27216

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: NA

Important sites: ACT_SITE 13-13

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEERVITWIGNGADVLMER
CCCHHHHCCEEEECCCEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
ALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYYGEVAEEGTFLFPHVADTL
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHH
GALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGGDDVQNKKPHPDPLLLVAERM
HHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
GIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEAIDLSQPDVIYQSINDLLPALG
CCCHHHEEEECCCCCHHHHHHHCCCCCCCCEECCCCCCEECCCCCHHHHHHHHHHHHHHC
LPHSENQESKND
CCCCCCCCCCCC
>Mature Secondary Structure 
SKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEERVITWIGNGADVLMER
CCHHHHCCEEEECCCEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
ALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYYGEVAEEGTFLFPHVADTL
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHH
GALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGGDDVQNKKPHPDPLLLVAERM
HHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
GIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEAIDLSQPDVIYQSINDLLPALG
CCCHHHEEEECCCCCHHHHHHHCCCCCCCCEECCCCCCEECCCCCHHHHHHHHHHHHHHC
LPHSENQESKND
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796