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The map label for this gene is yhfX [H]

Identifier: 157162856

GI number: 157162856

Start: 3554158

End: 3555321

Strand: Reverse

Name: yhfX [H]

Synonym: EcHS_A3576

Alternate gene names: 157162856

Gene position: 3555321-3554158 (Counterclockwise)

Preceding gene: 157162857

Following gene: 157162855

Centisome position: 76.56

GC content: 54.81

Gene sequence:

>1164_bases
ATGTTTGTAGAAGCATTGAAACGCCAGAACCCGGCGCTGATTTCCGCCGCACTAAGCCTGTGGCAGCAGGGCAAGATCGC
CCCGGACAGCTGGGTGATCGACGTGGATCAGATACTGGAAAACGGTAAGCGGCTGATTGAGGCGGCGCGGCTTTACGGCA
TTGAACTGTATCTGATGACCAAGCAGTTTGGTCGTAATCCGTGGCTGGCGGAAAAATTGCTGGCATTAGGCTACAGCGGC
ATTGTGGCGGTGGATTACAAAGAGGCGCGAGTCATGCGCCGCGCTGGTTTGCCTGTGGCGCATCAGGGGCATCTGGTACA
AATCCCTTGTCATCAGGTTGCTGACGCCGTTGAACAGGGCACCGACGTCATCACCGTGTTTACTCTCGACAAAGCGCGGG
AAGTTTCTGCGGCGGCGGTGAAGGCCGGGCGAATCCAGTCTGTGCTGCTTAAAGTTTATAGCGACGATGATTTTCTTTAT
CCGGGCCAGGAGAGCGGTTTTGCCCTCAAGGTGTTGCCGGAGATTGTCGCCGAAATCCAGAATCTGCCAGGGCTGCATTT
AGCCGGACTTACCCATTTCCCTTGCCTGCTTTGGGATGAGGCTGTCGGAAAAGTTTTGCCGACACCGAATCTTCACACGC
TGATACAGGCACGGGATCAACTGGCGAAATCTGGTATTGCACTTGAGCAACTGAACGCGCCTTCAGCGACCAGCTGCACT
TCGCTGCCATTACTGGCGCAATACGGTGTGACTCATGCCGAACCCGGTCATGCGCTGACGGGCACTATTCCGGCAAACCA
GCAGGGCGATCAGCCTGAACGTATCGCGATGCTCTGGTTAAGTGAAATCTCCCATCATTTCCGTGGCGACAGCTACTGCT
ACGGCGGCGGTTACTATCGTCGTGGTCATGCGCAACATGCGCTGGTGTTTACGCCAGAAAATCAAAAGATTACTGAAACC
AATCTCAAAACTGTGGATGACAGCAGTATCGACTACACCCTGCCGCTGGCAGGCGAGTATCCGGTGAGCAGTGCAGTGGT
GCTCTGTTTCCGCACGCAGATTTTTGTCACCCGTAGCGATGTGGTGCTGGTGTCCGGTATTCACCGTGGCGAACCGAAAA
TCGTTGGTCGTTATGACAGTCTTGGAAACTCTCTGGGGGCGTAA

Upstream 100 bases:

>100_bases
CTAACGAAGAGGTTTGAAGCTCCCGACAAATCCACGTCGCACAACCTAAATATTCAAAAAAATGAATATTTAATTCAAGG
AACAGAAACGAGGCAACAAG

Downstream 100 bases:

>100_bases
TGGCGCGATTTGTGGTGTTAGTGATTGATAGCTTTGGCGTAGGGGCAATGAAAGATGTCACGCTGGTGCGTCCGCAAGAT
GCGGGAGCGAATACCTGTGG

Product: alanine racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKRLIEAARLYGIELYLMTKQFGRNPWLAEKLLALGYSG
IVAVDYKEARVMRRAGLPVAHQGHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRIQSVLLKVYSDDDFLY
PGQESGFALKVLPEIVAEIQNLPGLHLAGLTHFPCLLWDEAVGKVLPTPNLHTLIQARDQLAKSGIALEQLNAPSATSCT
SLPLLAQYGVTHAEPGHALTGTIPANQQGDQPERIAMLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITET
NLKTVDDSSIDYTLPLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA

Sequences:

>Translated_387_residues
MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKRLIEAARLYGIELYLMTKQFGRNPWLAEKLLALGYSG
IVAVDYKEARVMRRAGLPVAHQGHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRIQSVLLKVYSDDDFLY
PGQESGFALKVLPEIVAEIQNLPGLHLAGLTHFPCLLWDEAVGKVLPTPNLHTLIQARDQLAKSGIALEQLNAPSATSCT
SLPLLAQYGVTHAEPGHALTGTIPANQQGDQPERIAMLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITET
NLKTVDDSSIDYTLPLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA
>Mature_387_residues
MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKRLIEAARLYGIELYLMTKQFGRNPWLAEKLLALGYSG
IVAVDYKEARVMRRAGLPVAHQGHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRIQSVLLKVYSDDDFLY
PGQESGFALKVLPEIVAEIQNLPGLHLAGLTHFPCLLWDEAVGKVLPTPNLHTLIQARDQLAKSGIALEQLNAPSATSCT
SLPLLAQYGVTHAEPGHALTGTIPANQQGDQPERIAMLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITET
NLKTVDDSSIDYTLPLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA

Specific function: Unknown

COG id: COG3457

COG function: function code E; Predicted amino acid racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789782, Length=387, Percent_Identity=99.2248062015504, Blast_Score=791, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001608 [H]

Pfam domain/function: PF01168 Ala_racemase_N [H]

EC number: NA

Molecular weight: Translated: 42280; Mature: 42280

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKRLIEAARLYGIELYLMT
CCHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHEEEEEEEE
KQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQGHLVQIPCHQVADAVEQG
HHHCCCCCHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCEECCCCEEEECHHHHHHHHHCC
TDVITVFTLDKAREVSAAAVKAGRIQSVLLKVYSDDDFLYPGQESGFALKVLPEIVAEIQ
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHHEECCCCEECCCCCCCEEEHHHHHHHHHHH
NLPGLHLAGLTHFPCLLWDEAVGKVLPTPNLHTLIQARDQLAKSGIALEQLNAPSATSCT
CCCCCEECCHHHCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCC
SLPLLAQYGVTHAEPGHALTGTIPANQQGDQPERIAMLWLSEISHHFRGDSYCYGGGYYR
CCCHHHHCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCEEE
RGHAQHALVFTPENQKITETNLKTVDDSSIDYTLPLAGEYPVSSAVVLCFRTQIFVTRSD
CCCCCEEEEECCCCCEEECCCCEECCCCCCEEEEECCCCCCHHHHEEEEEEEEEEEEECC
VVLVSGIHRGEPKIVGRYDSLGNSLGA
EEEEECCCCCCCCEEECHHHHCCCCCC
>Mature Secondary Structure
MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKRLIEAARLYGIELYLMT
CCHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHEEEEEEEE
KQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQGHLVQIPCHQVADAVEQG
HHHCCCCCHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCEECCCCEEEECHHHHHHHHHCC
TDVITVFTLDKAREVSAAAVKAGRIQSVLLKVYSDDDFLYPGQESGFALKVLPEIVAEIQ
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHHEECCCCEECCCCCCCEEEHHHHHHHHHHH
NLPGLHLAGLTHFPCLLWDEAVGKVLPTPNLHTLIQARDQLAKSGIALEQLNAPSATSCT
CCCCCEECCHHHCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCC
SLPLLAQYGVTHAEPGHALTGTIPANQQGDQPERIAMLWLSEISHHFRGDSYCYGGGYYR
CCCHHHHCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCEEE
RGHAQHALVFTPENQKITETNLKTVDDSSIDYTLPLAGEYPVSSAVVLCFRTQIFVTRSD
CCCCCEEEEECCCCCEEECCCCEECCCCCCEEEEECCCCCCHHHHEEEEEEEEEEEEECC
VVLVSGIHRGEPKIVGRYDSLGNSLGA
EEEEECCCCCCCCEEECHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]