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The map label for this gene is frlD [H]

Identifier: 157162849

GI number: 157162849

Start: 3547512

End: 3548297

Strand: Direct

Name: frlD [H]

Synonym: EcHS_A3569

Alternate gene names: 157162849

Gene position: 3547512-3548297 (Clockwise)

Preceding gene: 157162848

Following gene: 157162850

Centisome position: 76.4

GC content: 57.38

Gene sequence:

>786_bases
ATGAAAACCCTGGCGACAATCGGCGATAACTGCGTCGATATCTACCCGCAACTGAATAAAGCGTTTTCTGGCGGTAATGC
GGTCAATGTGGCGGTGTACTGCACTCGCTACGGCATACAGCCGGGATGCATTACCTGGGTGGGTGACGATGACTACGGCA
CAAAGCTGAAGCAGGATCTCGCCCGCATGGGCGTCGATATCAGCCATGTCCATACGAAACACGGCGTTACCGCACAAACT
CAGGTGGAACTGCACGACAATGATCGCGTTTTTGGCGACTACACCGAAGGCGTGATGGCCGACTTTGCCCTGAGTGAAGA
GGATTACGCCTGGCTGGCGCAGTATGACATTGTGCACGCCGCAATCTGGGGACATGCGGAAGACGCATTCCCACAGCTGC
ACGCTGCGGGCAAACTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTC
GATTTTGCCTTTGCCTCCGCACCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGG
AACAGTGATTGTCACGCTGGGTGAAAACGGCAGCATTGCCTGGGATGGCGCGCAGTTCTGGCGTCAGGCTCCTGAACCGG
TGACGGTTATCGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTA
CCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAG

Upstream 100 bases:

>100_bases
ACTGTACGGTGGAGCTGGTGACGATGTATATGAACGAGCCCAGACTCTATGCCCGCCAGGCGCTGGAACGCTTTCGCGCG
CTGCTGCCGGAGGATGAGAG

Downstream 100 bases:

>100_bases
GTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATT
CTTTATTTCAGGGTCGATTA

Product: fructoselysine 6-kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCITWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQT
QVELHDNDRVFGDYTEGVMADFALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVIDTMGAGDSFIAGFLCGWSAGMTL
PQAIAQGTACAAKTIQYHGAW

Sequences:

>Translated_261_residues
MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCITWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQT
QVELHDNDRVFGDYTEGVMADFALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVIDTMGAGDSFIAGFLCGWSAGMTL
PQAIAQGTACAAKTIQYHGAW
>Mature_261_residues
MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCITWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQT
QVELHDNDRVFGDYTEGVMADFALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVIDTMGAGDSFIAGFLCGWSAGMTL
PQAIAQGTACAAKTIQYHGAW

Specific function: Phosphorylates fructoselysine to yield fructoselysine 6- phosphate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1789775, Length=261, Percent_Identity=100, Blast_Score=539, Evalue=1e-155,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: NA

Molecular weight: Translated: 28332; Mature: 28332

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00583 PFKB_KINASES_1 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCITWVGDDDYGTKLKQDL
CCCHHHCCCCCEEECHHHHHHCCCCCEEEEEEEEEECCCCCCEEEEECCCCCCHHHHHHH
ARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADFALSEEDYAWLAQYDIVHA
HHHCCCHHHHHHCCCCCEEEEEEECCCCCEECCHHHHHHHHHCCCCCCCCHHHHHHHHHH
AIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHLDFAFASAPQEDETLRLKMKA
HHHCCCCHHCHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHEEEEE
IVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVIDTMGAGDSFIAGFLCGWSAGMTL
HHCCCCCEEEEEECCCCCEEECCHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCH
PQAIAQGTACAAKTIQYHGAW
HHHHHCCCHHHHHHEEECCCC
>Mature Secondary Structure
MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCITWVGDDDYGTKLKQDL
CCCHHHCCCCCEEECHHHHHHCCCCCEEEEEEEEEECCCCCCEEEEECCCCCCHHHHHHH
ARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADFALSEEDYAWLAQYDIVHA
HHHCCCHHHHHHCCCCCEEEEEEECCCCCEECCHHHHHHHHHCCCCCCCCHHHHHHHHHH
AIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHLDFAFASAPQEDETLRLKMKA
HHHCCCCHHCHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHEEEEE
IVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVIDTMGAGDSFIAGFLCGWSAGMTL
HHCCCCCEEEEEECCCCCEEECCHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCH
PQAIAQGTACAAKTIQYHGAW
HHHHHCCCHHHHHHEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]