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The map label for this gene is nirD

Identifier: 157162842

GI number: 157162842

Start: 3540895

End: 3541221

Strand: Direct

Name: nirD

Synonym: EcHS_A3562

Alternate gene names: 157162842

Gene position: 3540895-3541221 (Clockwise)

Preceding gene: 157162841

Following gene: 157162843

Centisome position: 76.25

GC content: 54.74

Gene sequence:

>327_bases
ATGAGCCAGTGGAAAGACATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCA
GGTAGCGATTTTCCGCCCGTATCACAGCGACCAGGTGTTTGCGATCAGCAACATCGATCCGTTCTTCGAGTCCAGCGTGC
TATCACGCGGGCTGATTGCGGAACACCAGGGCGAGCTGTGGGTCGCCAGCCCGCTGAAAAAACAGCGTTTTCGCTTAAGT
GATGGCTTGTGCATGGAAGACGAACAGTTTTCCGTCAAACATTACGAAGCACGAGTGAAAGACGGCGTGGTGCAGCTGCG
CGGTTAA

Upstream 100 bases:

>100_bases
ACAAGCGTGACCCGAACGTGCAGATGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCGGTA
ACTCTGGTGGAGGACAACGC

Downstream 100 bases:

>100_bases
TGTTTTAACGGGAGGCGCAATGCCTCCCCTTTTTGCATGGTCCTGTAATGACCTTCGGTATATTGCAGGACATTTTTTAA
ACTTTTTGTTTTATTTTTTT

Product: nitrite reductase small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 108; Mature: 107

Protein sequence:

>108_residues
MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISNIDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLS
DGLCMEDEQFSVKHYEARVKDGVVQLRG

Sequences:

>Translated_108_residues
MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISNIDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLS
DGLCMEDEQFSVKHYEARVKDGVVQLRG
>Mature_107_residues
SQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISNIDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSD
GLCMEDEQFSVKHYEARVKDGVVQLRG

Specific function: Required for activity of the reductase

COG id: COG2146

COG function: function code PR; Ferredoxin subunits of nitrite reductase and ring-hydroxylating dioxygenases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To B.subtilis nasE

Homologues:

Organism=Escherichia coli, GI1789766, Length=108, Percent_Identity=100, Blast_Score=224, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIRD_ECO57 (P0A9I9)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A91156
- PIR:   F86001
- RefSeq:   NP_289914.1
- RefSeq:   NP_312244.1
- ProteinModelPortal:   P0A9I9
- SMR:   P0A9I9
- EnsemblBacteria:   EBESCT00000024716
- EnsemblBacteria:   EBESCT00000060215
- GeneID:   915916
- GeneID:   958830
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4727
- KEGG:   ecs:ECs4217
- GeneTree:   EBGT00050000011425
- HOGENOM:   HBG668715
- OMA:   EDERFNV
- ProtClustDB:   PRK09511
- BioCyc:   ECOL83334:ECS4217-MONOMER
- GO:   GO:0005737
- InterPro:   IPR012748
- InterPro:   IPR017881
- InterPro:   IPR017941
- Gene3D:   G3DSA:2.102.10.10
- TIGRFAMs:   TIGR02378

Pfam domain/function: SSF50022 Rieske_dom

EC number: =1.7.1.4

Molecular weight: Translated: 12284; Mature: 12153

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS51300 NIRD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISNIDPFFESSVLSRGLIA
CCCCHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCCCEEEECCCCCHHHHHHHHCCCCC
EHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDGVVQLRG
CCCCCEEEECCCCHHHCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECC
>Mature Secondary Structure 
SQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISNIDPFFESSVLSRGLIA
CCCHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCCCEEEECCCCCHHHHHHHHCCCCC
EHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDGVVQLRG
CCCCCEEEECCCCHHHCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796