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The map label for this gene is dsrH

Identifier: 157162819

GI number: 157162819

Start: 3519021

End: 3519308

Strand: Reverse

Name: dsrH

Synonym: EcHS_A3539

Alternate gene names: 157162819

Gene position: 3519308-3519021 (Counterclockwise)

Preceding gene: 157162820

Following gene: 157162818

Centisome position: 75.79

GC content: 50.35

Gene sequence:

>288_bases
ATGCTGCACACGTTACATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGAAGGAGACGAACT
GCTATTATTGCAAGATGGCGTAACTGCCGCAGTTGACGGTAACCGCTACCTTGAAAGTCTGCGTAATGCCCCCATTAAGG
TCTATGCCCTGAACGAAGACCTTATTGCCCGCGGTTTGACTGGTCAAATTTCGAACGACATCATTCCCATTGACTATACT
GATTTCGTCAGACTTACGGTTAAGCACTCCAGCCAGATGGCCTGGTGA

Upstream 100 bases:

>100_bases
CCGCAGACACCCTTTGTTGTCGAAGCCACGCCGCTCGAAGCAGATGCCTTACGCCGCGAACTCGCCAACTACGATGTTAT
TTTGAGGTTTTGAGGCGTTT

Downstream 100 bases:

>100_bases
TGGCGGGATCGTTGTATATTTCTTGACACCTTTTCGGCATCGCCCTAAAATTCGGCGTCCTCATATTGTGTGAGGACGTT
TTATTACGTGTTTACGAAGC

Product: sulfur transfer complex subunit TusB

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein B

Number of amino acids: Translated: 95; Mature: 95

Protein sequence:

>95_residues
MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYLESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYT
DFVRLTVKHSSQMAW

Sequences:

>Translated_95_residues
MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYLESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYT
DFVRLTVKHSSQMAW
>Mature_95_residues
MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYLESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYT
DFVRLTVKHSSQMAW

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions

COG id: COG2168

COG function: function code P; Uncharacterized conserved protein involved in oxidation of intracellular sulfur

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dsrH/tusB family

Homologues:

Organism=Escherichia coli, GI1789741, Length=95, Percent_Identity=97.8947368421053, Blast_Score=187, Evalue=8e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TUSB_ECO24 (A7ZSL8)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001464797.1
- ProteinModelPortal:   A7ZSL8
- SMR:   A7ZSL8
- STRING:   A7ZSL8
- EnsemblBacteria:   EBESCT00000021425
- GeneID:   5586512
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_3812
- eggNOG:   COG2168
- GeneTree:   EBGT00050000011785
- HOGENOM:   HBG682569
- OMA:   SPWHADI
- ProtClustDB:   PRK13510
- BioCyc:   ECOL331111:ECE24377A_3812-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01564
- InterPro:   IPR007215
- TIGRFAMs:   TIGR03011

Pfam domain/function: PF04077 DsrH

EC number: NA

Molecular weight: Translated: 10692; Mature: 10692

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYLESLRNAPIKVYALNED
CCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCEEEECCHHHHHHHCCCCEEEEEECHH
LIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW
HHHCCCCCCCCCCEECCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYLESLRNAPIKVYALNED
CCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCEEEECCHHHHHHHCCCCEEEEEECHH
LIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW
HHHCCCCCCCCCCEECCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA