Click here to switch to the map view.

The map label for this gene is gspO [H]

Identifier: 157162809

GI number: 157162809

Start: 3509886

End: 3510563

Strand: Direct

Name: gspO [H]

Synonym: EcHS_A3529

Alternate gene names: 157162809

Gene position: 3509886-3510563 (Clockwise)

Preceding gene: 157162808

Following gene: 157162824

Centisome position: 75.59

GC content: 50.44

Gene sequence:

>678_bases
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCACCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Upstream 100 bases:

>100_bases
CACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTG
TCCTTCAGGGAGCAACAATA

Downstream 100 bases:

>100_bases
CCGGGAGGGTTCTCCCTCCCGACACGGCTCAACCTTCTTCGCGGATCTGTGCTTGCAGATAATTTTGCAGGCCCATCTTC
TGAATCAGATCAAGTTCCGT

Product: type 4 prepilin peptidase

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFHQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Sequences:

>Translated_225_residues
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFHQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY
>Mature_224_residues
TMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFHQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALF
LYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFKL
LAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI1789732, Length=225, Percent_Identity=99.1111111111111, Blast_Score=442, Evalue=1e-126,
Organism=Escherichia coli, GI87082194, Length=140, Percent_Identity=39.2857142857143, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 24880; Mature: 24749

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFHQVLVHFRQKKYAWHDTVPLILC
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHCHHHHHHH
VAAAIACALAPFTPIVTGALFLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYG
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
LIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFKLLAAAGAWCGWQTLPMILLI
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHH
ASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFHQVLVHFRQKKYAWHDTVPLILC
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHCHHHHHHH
VAAAIACALAPFTPIVTGALFLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYG
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
LIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFKLLAAAGAWCGWQTLPMILLI
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHH
ASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7959070; 9278503; 2661540; 1676385 [H]