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The map label for this gene is pioO [H]

Identifier: 157162796

GI number: 157162796

Start: 3497851

End: 3498270

Strand: Reverse

Name: pioO [H]

Synonym: EcHS_A3516

Alternate gene names: 157162796

Gene position: 3498270-3497851 (Counterclockwise)

Preceding gene: 157162797

Following gene: 157162795

Centisome position: 75.34

GC content: 46.19

Gene sequence:

>420_bases
ATGTTTGAATTCTATATCGCCGCGCGTGAGCAGAAAGAAACAGGTCACCCTGGTATCTTCTCCCGACAAAAACACAGCAC
CATAATATATGTAATTTGTTTATTACTGATTTGCCTGTGGTTTGCAGGAATGGTGCTGGTTGGTGGGTATGCCAGGCAGC
TATGGGTGCTTTGGATAGTAAAAGCCGAAGTCACTGTAGAGGCTGAAACGCCCGCTTTTAAACAATCAACACAACACTAT
TTCTTCAAAAAACAGCCACTCCCCGTCGTCGAATCCGTCGAGGAGGAAGATGACCCAGGCGTCGCGGTTGAGAATGCCCC
CTCTTCTTCTGAAGACGAAGAAAATACTGTGGAAGAAAGCGACGAGAAAGCCGGTTTAAGAGAGCGTGTCAAAAATGCAC
TGAACGAACTTGAGAGATAA

Upstream 100 bases:

>100_bases
TCAACGTTAGTACATCTCTGGCAGGTGGCAGGGGAGAGTGCCTACTTATATCGGGATGAAGCAAATATTTCCCCGGAAAC
AACAGTGAAGGGGAAATGAC

Downstream 100 bases:

>100_bases
CCCGAAGGCTGTTTACTTTACTAGCAATACGCTTGCGTTCGGTGGTTAAGTATGTATAATGCGCGGGCTTGTCGTAGTTG
ACAGCAGGTTCAATCTGAAC

Product: chromosome replication protein PioO

Products: NA

Alternate protein names: Protein pinO [H]

Number of amino acids: Translated: 139; Mature: 139

Protein sequence:

>139_residues
MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGMVLVGGYARQLWVLWIVKAEVTVEAETPAFKQSTQHY
FFKKQPLPVVESVEEEDDPGVAVENAPSSSEDEENTVEESDEKAGLRERVKNALNELER

Sequences:

>Translated_139_residues
MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGMVLVGGYARQLWVLWIVKAEVTVEAETPAFKQSTQHY
FFKKQPLPVVESVEEEDDPGVAVENAPSSSEDEENTVEESDEKAGLRERVKNALNELER
>Mature_139_residues
MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGMVLVGGYARQLWVLWIVKAEVTVEAETPAFKQSTQHY
FFKKQPLPVVESVEEEDDPGVAVENAPSSSEDEENTVEESDEKAGLRERVKNALNELER

Specific function: Calcium-binding protein that may be required for the initiation of chromosome replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789719, Length=139, Percent_Identity=99.2805755395683, Blast_Score=285, Evalue=8e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 15862; Mature: 15862

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGMVLVGGYARQLWVLWIV
CCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
KAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPGVAVENAPSSSEDEENTVEES
EHEEEEECCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCEEECCCCCCCCCHHHHHHC
DEKAGLRERVKNALNELER
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGMVLVGGYARQLWVLWIV
CCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
KAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPGVAVENAPSSSEDEENTVEES
EHEEEEECCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCEEECCCCCCCCCHHHHHHC
DEKAGLRERVKNALNELER
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7037196; 9278503; 1925011; 1938934 [H]