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The map label for this gene is acrE

Identifier: 157162746

GI number: 157162746

Start: 3458207

End: 3459364

Strand: Direct

Name: acrE

Synonym: EcHS_A3458

Alternate gene names: 157162746

Gene position: 3458207-3459364 (Clockwise)

Preceding gene: 157162744

Following gene: 157162747

Centisome position: 74.47

GC content: 52.42

Gene sequence:

>1158_bases
ATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCCGGTTGTAACGATAA
GGGAGAAGAGAAAGCTCACGTCGGTGAACCGCAGGTTACCGTTCATATTGTGAAAACGGCCCCGTTAGAAGTTAAGACTG
AATTACCAGGCCGCACCAATGCTTACCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGATCGTACTGAATCGTAATTTC
ACTGAAGGCAGCGATGTACAAGCAGGCCAGTCCCTGTACCAGATCGATCCCGCGACCTATCAGGCAAATTATGACAGCGC
GAAAGGCGAACTGGCGAAAAGTGAAGCCGCCGCCGCCATCGCGCATTTGACGGTAAAACGTTACGTTCCGCTCGTGGGTA
CGAAATACATCAGCCAGCAGGAGTACGACCAGGCCATTGCTGATGCTCGCCAGGCCGATGCCGCCGTGATTGCCGCAAAA
GCCACAGTCGAAAGCGCTCGCATCAATCTCGCTTATACCAAAGTGACTGCGCCAATTAGCGGACGTATCGGCAAATCGAC
CGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTG
ATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACC
AGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCTGATGTGACCGTTGA
TGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTG
CACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCA
ACCGTGCTGATTGTTAACGATAAAAGTCAGGTGGAAGCGCGCCCTGTCGTTGCCAGTCAGGCGATTGGCGATAAATGGTT
GATTAGCGAAGGTCTGAAATCTGGCGATCAAGTCATTGTCAGCGGCCTGCAAAAAGCGCGTCCGGGAGAGCAGGTTAAAG
CCACTACCGATACCCCCGCAGATACTGCATCGAAGTAA

Upstream 100 bases:

>100_bases
ATGATAATTTGTAGGATAGCAAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTT
TGGTTTTTTGAGGAATAGTA

Downstream 100 bases:

>100_bases
GGTAATCTGACATGGCAAACTTTTTTATTCGACGACCGATATTTGCATGGGTGCTGGCCATTATTCTGATGATGGCGGGC
GCACTGGCGATCCTACAATT

Product: acriflavine resistance protein E

Products: NA

Alternate protein names: Protein envC

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIVKTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNF
TEGSDVQAGQSLYQIDPATYQANYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQSSNDFMRLKQSVEQGNLHKENAT
SNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLRAVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDA
TVLIVNDKSQVEARPVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK

Sequences:

>Translated_385_residues
MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIVKTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNF
TEGSDVQAGQSLYQIDPATYQANYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQSSNDFMRLKQSVEQGNLHKENAT
SNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLRAVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDA
TVLIVNDKSQVEARPVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK
>Mature_384_residues
TKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIVKTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFT
EGSDVQAGQSLYQIDPATYQANYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAKA
TVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQSSNDFMRLKQSVEQGNLHKENATS
NVELVMENGQTYPLKGTLQFSDVTVDESTGSITLRAVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDAT
VLIVNDKSQVEARPVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK

Specific function: May affect specific membrane functions, such as septum formation during cell division, and cell membrane permeability

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family

Homologues:

Organism=Escherichia coli, GI1789665, Length=385, Percent_Identity=100, Blast_Score=785, Evalue=0.0,
Organism=Escherichia coli, GI1786668, Length=375, Percent_Identity=69.3333333333333, Blast_Score=508, Evalue=1e-145,
Organism=Escherichia coli, GI1789929, Length=382, Percent_Identity=49.4764397905759, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI87082036, Length=320, Percent_Identity=29.0625, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI87081796, Length=205, Percent_Identity=27.3170731707317, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ACRE_ECOLI (P24180)

Other databases:

- EMBL:   M96848
- EMBL:   X57948
- EMBL:   U18997
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   C65119
- RefSeq:   AP_003805.1
- RefSeq:   NP_417731.1
- ProteinModelPortal:   P24180
- SMR:   P24180
- STRING:   P24180
- EnsemblBacteria:   EBESCT00000001673
- EnsemblBacteria:   EBESCT00000016918
- GeneID:   947706
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3233
- KEGG:   eco:b3265
- EchoBASE:   EB0262
- EcoGene:   EG10266
- eggNOG:   COG0845
- GeneTree:   EBGT00050000009284
- HOGENOM:   HBG742930
- OMA:   SRNFTEG
- ProtClustDB:   CLSK880627
- BioCyc:   EcoCyc:EG10266-MONOMER
- Genevestigator:   P24180
- InterPro:   IPR006143
- TIGRFAMs:   TIGR01730

Pfam domain/function: PF00529 HlyD

EC number: NA

Molecular weight: Translated: 41318; Mature: 41187

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIVKTAPLEVKTELPGRTN
CCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCEEEEECCCCCCC
AYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQANYDSAKGELAKSEAAAAI
CEEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEECCCEEECCCCCCCCHHHHHHHHHHH
AHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAKATVESARINLAYTKVTAPIS
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEEEEEEEEEEEEECCCC
GRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQSSNDFMRLKQSVEQGNLHKENAT
CCCCCCCCCCCEEEECCCCCEEEHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCC
SNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLRAVFPNPQHTLLPGMFVRARIDEGV
CCEEEEEECCCEECEEEEEEEEEEEEECCCCCEEEEEECCCCCCCCCCCEEEEEECCCCC
QPDAILIPQQGVSRTPRGDATVLIVNDKSQVEARPVVASQAIGDKWLISEGLKSGDQVIV
CCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHCCCHHHHHHHCCCCHHHHH
SGLQKARPGEQVKATTDTPADTASK
HHHHHCCCCCCEEECCCCCCCCCCC
>Mature Secondary Structure 
TKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIVKTAPLEVKTELPGRTN
CCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCEEEEECCCCCCC
AYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQANYDSAKGELAKSEAAAAI
CEEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEECCCEEECCCCCCCCHHHHHHHHHHH
AHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAKATVESARINLAYTKVTAPIS
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEEEEEEEEEEEEECCCC
GRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQSSNDFMRLKQSVEQGNLHKENAT
CCCCCCCCCCCEEEECCCCCEEEHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCC
SNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLRAVFPNPQHTLLPGMFVRARIDEGV
CCEEEEEECCCEECEEEEEEEEEEEEECCCCCEEEEEECCCCCCCCCCCEEEEEECCCCC
QPDAILIPQQGVSRTPRGDATVLIVNDKSQVEARPVVASQAIGDKWLISEGLKSGDQVIV
CCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHCCCHHHHHHHCCCCHHHHH
SGLQKARPGEQVKATTDTPADTASK
HHHHHCCCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1720861; 9278503