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The map label for this gene is envR

Identifier: 157162745

GI number: 157162745

Start: 3457146

End: 3457808

Strand: Reverse

Name: envR

Synonym: EcHS_A3457

Alternate gene names: 157162745

Gene position: 3457808-3457146 (Counterclockwise)

Preceding gene: 157162754

Following gene: 157162730

Centisome position: 74.46

GC content: 44.19

Gene sequence:

>663_bases
ATGGCAAAAAGAACCAAAGCCGAAGCTCTGAAGACCCGGCAAGAACTGATTGAAACTGCCATCGCCCAGTTTGCGCAACA
TGGCGTAAGCAAGACGACGCTCAACGACATTGCCGATGCCGCTAACGTTACGCGTGGCGCTATCTACTGGCACTTCGAGA
ACAAGACCCAACTGTTTAATGAGATGTGGTTGCAACAGCCTTCATTGCGGGAGTTAATCCAGGAACACTTGACGGCTGGA
TTAGAGCATGACCCGTTTCAACAATTGCGTGAAAAATTGATTGTCGGCTTGCAATATATTGCCAAAATTCCCCGCCAGCA
GGCGTTGCTGAAAATCTTATATCACAAATGTGAATTTAATGATGAGATGCTGGCCGAGGGAGTGATACGCGAAAAGATGG
GCTTTAATCCGCAGACTCTCCGCGAAGTATTGCAGGCGTGTCAGCAACAAGGTTGTGTAGCAAATAACCTCGATTTAGAT
GTTGTGATGATTATTATTGATGGTGCCTTCAGCGGAATTGTTCAAAACTGGTTAATGAATATGGCGGGTTATGATCTTTA
TAAACAAGCACCCGCGCTGGTCGATAACGTATTAAGAATGTTCATGCCAGATGAAAACATAACGAAATTAATTCATCAAA
CGAATGAATTAAGTGTCATGTAA

Upstream 100 bases:

>100_bases
TCAATGTTACCCTCTCGTATAAATACACATTAGATGATAGATTAACCTTCGCTATTTTCTCACCCTGTGTCGAATATATT
TATTTCCTGAATAATTAATC

Downstream 100 bases:

>100_bases
ATTCACATTCTGTCCTGAAAATTCAGTTCGTCACAGTTAACCAGTTTTAGTGGATTAACTGAGTTCATATTACGGCATTT
GTCTGTTTCTGTTGCCAGCA

Product: DNA-binding transcriptional regulator EnvR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVTRGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAG
LEHDPFQQLREKLIVGLQYIAKIPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELSVM

Sequences:

>Translated_220_residues
MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVTRGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAG
LEHDPFQQLREKLIVGLQYIAKIPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELSVM
>Mature_219_residues
AKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVTRGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGL
EHDPFQQLREKLIVGLQYIAKIPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLDV
VMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELSVM

Specific function: Potential regulator protein for the acrEF/envCD genes

COG id: COG1309

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1789664, Length=220, Percent_Identity=100, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI1786669, Length=204, Percent_Identity=38.2352941176471, Blast_Score=134, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ENVR_ECO57 (P0ACT3)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D85991
- PIR:   H91145
- RefSeq:   NP_289832.1
- RefSeq:   NP_312163.1
- ProteinModelPortal:   P0ACT3
- SMR:   P0ACT3
- EnsemblBacteria:   EBESCT00000027868
- EnsemblBacteria:   EBESCT00000055574
- GeneID:   916016
- GeneID:   958706
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4624
- KEGG:   ecs:ECs4136
- GeneTree:   EBGT00050000009447
- HOGENOM:   HBG701145
- OMA:   REKIGFH
- ProtClustDB:   PRK09975
- BioCyc:   ECOL83334:ECS4136-MONOMER
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR013572
- InterPro:   IPR001647
- Gene3D:   G3DSA:1.10.10.60
- Gene3D:   G3DSA:1.10.357.10
- PRINTS:   PR00455

Pfam domain/function: PF08361 TetR_C_2; PF00440 TetR_N; SSF46689 Homeodomain_like; SSF48498 TetR_like_C

EC number: NA

Molecular weight: Translated: 25199; Mature: 25067

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS01081 HTH_TETR_1; PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVTRGAIYWHFENKTQLFN
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCEEEEEECCHHHHHH
EMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAKIPRQQALLKILYHKCEFN
HHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
DEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLDVVMIIIDGAFSGIVQNWLMN
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
MAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELSVM
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVTRGAIYWHFENKTQLFN
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCEEEEEECCHHHHHH
EMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAKIPRQQALLKILYHKCEFN
HHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
DEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLDVVMIIIDGAFSGIVQNWLMN
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
MAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELSVM
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796