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The map label for this gene is csrD

Identifier: 157162730

GI number: 157162730

Start: 3444980

End: 3446920

Strand: Reverse

Name: csrD

Synonym: EcHS_A3442

Alternate gene names: 157162730

Gene position: 3446920-3444980 (Counterclockwise)

Preceding gene: 157162745

Following gene: 157162728

Centisome position: 74.23

GC content: 50.7

Gene sequence:

>1941_bases
ATGAGATTAACGACGAAATTTTCGGCCTTTGTTACGCTGCTCACCGGGTTAACAATTTTTGTGACTTTGCTGGGCTGTTC
GCTAAGTTTCTACAACGCCATTCAGTATAAGTTTAGTCATCGCGTTCAGGCGGTGGCGACGGCGATTGATACCCACCTTG
TGTCGAATGACTTCAGCGTATTAAGGCCACAAATTACCGAATTAATGATGTCGGCAGATATCGTTCGTGTAGACCTGCTC
CATGGTGATAAACAGGTTTATACCCTGGCCAGAAATGGTAGTTATCGTCCAGTTGGCTCCAGCGATCTGTTTCGCGAACT
GAGCGTTCCGTTGATAAAGCATCCGGGGATGTCGTTGCGTCTGGTTTATCAGGATCCGATGGGCAACTATTTCCATTCGT
TGATGACCACCGCGCCGCTCACGGGGGCGATTGGCTTTATCATTGTTATGCTCTTCCTGGCGGTACGCTGGTTACAACGG
CAACTTGCCGGGCAAGAATTGCTGGAAACCCGGGCTACTCGTATCTTAAACGGTGAGCGTGGCTCTAATGTGTTGGGAAC
CATCTATGAATGGCCGCCCAGAACCAGCAGTGCGCTGGATACGCTGCTTCGTGAAATTCAGAACGCACGCGAACAACACA
GCCGTCTTGATACGCTGATCCGCTCTTATGCCGCCCAGGACGTGAAAACCGGCCTCAATAACCGACTCTTTTTCGATAAT
CAGTTAGCAACGTTACTGGAAGATCAGGAGAAAGTAGGTACCCACGGGATCGTGATGATGATTCGTCTGCCGGATTTCAA
TATGTTGAGCGATACCTGGGGGCACAGCCAGGTTGAAGAACAGTTCTTCACTCTGACGAATCTGCTGTCGACATTTATGA
TGCGCTACCCTGGCGCACTGCTGGCGCGTTACCACCGCAGTGATTTTGCTGCGCTGTTACCGCACCGGACGTTAAAAGAG
GCAGAGAGCATCGCCGGTCAGTTAATCAAAGCCGTTGATACCTTGCCGAACAATAAAATGCTCGATCGCGACGATATGAT
CCACATTGGTATCTGCGCCTGGCGTAGTGGTCAGGATACCGAGCAGGTAATGGAACATGCAGAGTCTGCCACGCGTAATG
CGGGATTGCAGGGCGGCAATAGCTGGGCTATTTACGATGACTCGTTGCCTGAAAAAGGACGCGGTAATGTTCGCTGGCGT
ACGCTTATCGAGCAAATGCTCAGTCGCGGCGGCCCGCGCCTTTATCAAAAACCGGCGGTTACTCGCGAAGGTCAGGTTCA
TCATCGCGAACTCATGTGCCGCATCTTCGATGGTAATGAAGAGGTTAGCTCGGCGGAGTATATGCCGATGGTCTTGCAGT
TTGGCTTATCGGAAGAGTATGACCGTCTGCAAATCAGCCGTCTTATTCCACTATTGCGTTACTGGCCAGAGGAAAATCTG
GCGATTCAGGTTACCGTTGAGTCGCTGATTCGCCCGCGTTTTCAGCGTTGGCTGCGCGATACGTTAATGCAATGTGAAAA
ATCACAACGAAAACGCATAATTATTGAACTTGCAGAGGCCGATGTAGGTCAACATATCAGTCGTTTACAACCTGTTATTC
GTTTAGTGAATGCTTTAGGGGTACGGGTAGCCGTCAACCAGGCTGGTTTGACGCTGGTAAGTACCAGTTGGATCAAAGAA
CTTAATGTTGAGTTACTCAAGCTCCATCCGGGGCTGGTCAGAAACATTGAGAAGCGAACGGAGAACCAGCTGCTGGTTCA
AAGCCTGGTGGAAGCCTGCTCCGGGACCAGCACCCAGGTTTACGCCACCGGCGTGCGTTCGCGAAGCGAGTGGCAGACCC
TGATTCAGCGCGGTGTTACAGGCGGGCAAGGGGATTTTTTCGCGTCCTCACAGCCACTTGATACTAACGTGAAAAAATAT
TCACAAAGATACTCGGTTTAA

Upstream 100 bases:

>100_bases
CTCGCTAATGGAAAAGCAAAGCCGATGAAGCGCAATAAATTGCGAGATAAATCTGATTTGCTAGTATGCCCGCTTCCTCA
CTATCGGAGTTAACACAAGG

Downstream 100 bases:

>100_bases
CCTGCCGTTTAATCCGTTTTCACGTAGAATAACGCGCGCTGCGTCTCATGGGAGTGTGCTTGTCTGCTCGCCAGATTGTT
GCAGCACATATGCAGATGAA

Product: regulatory protein CsrD

Products: NA

Alternate protein names: Regulator of CsrB and CsrC decay CsrD

Number of amino acids: Translated: 646; Mature: 646

Protein sequence:

>646_residues
MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQAVATAIDTHLVSNDFSVLRPQITELMMSADIVRVDLL
HGDKQVYTLARNGSYRPVGSSDLFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDTLIRSYAAQDVKTGLNNRLFFDN
QLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHSQVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKE
AESIAGQLIKAVDTLPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEKGRGNVRWR
TLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPMVLQFGLSEEYDRLQISRLIPLLRYWPEENL
AIQVTVESLIRPRFQRWLRDTLMQCEKSQRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKE
LNVELLKLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQGDFFASSQPLDTNVKKY
SQRYSV

Sequences:

>Translated_646_residues
MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQAVATAIDTHLVSNDFSVLRPQITELMMSADIVRVDLL
HGDKQVYTLARNGSYRPVGSSDLFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDTLIRSYAAQDVKTGLNNRLFFDN
QLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHSQVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKE
AESIAGQLIKAVDTLPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEKGRGNVRWR
TLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPMVLQFGLSEEYDRLQISRLIPLLRYWPEENL
AIQVTVESLIRPRFQRWLRDTLMQCEKSQRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKE
LNVELLKLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQGDFFASSQPLDTNVKKY
SQRYSV
>Mature_646_residues
MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQAVATAIDTHLVSNDFSVLRPQITELMMSADIVRVDLL
HGDKQVYTLARNGSYRPVGSSDLFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDTLIRSYAAQDVKTGLNNRLFFDN
QLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHSQVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKE
AESIAGQLIKAVDTLPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEKGRGNVRWR
TLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPMVLQFGLSEEYDRLQISRLIPLLRYWPEENL
AIQVTVESLIRPRFQRWLRDTLMQCEKSQRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKE
LNVELLKLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQGDFFASSQPLDTNVKKY
SQRYSV

Specific function: Serves as a specificity factor required for RNase E- mediated decay of the small global regulatory RNAs CsrB and CsrC, it is probably not a nuclease. Nor does its activity involve c-di- GMP, despite its domain composition. Positively modulates motility ge

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain

Homologues:

Organism=Escherichia coli, GI1789650, Length=646, Percent_Identity=100, Blast_Score=1333, Evalue=0.0,
Organism=Escherichia coli, GI1787541, Length=437, Percent_Identity=21.5102974828375, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1788849, Length=236, Percent_Identity=22.8813559322034, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787410, Length=121, Percent_Identity=26.4462809917355, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CSRD_ECOLI (P13518)

Other databases:

- EMBL:   U18997
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   M22055
- PIR:   F65117
- RefSeq:   AP_003793.1
- RefSeq:   NP_417718.1
- ProteinModelPortal:   P13518
- DIP:   DIP-12292N
- MINT:   MINT-1286606
- STRING:   P13518
- PRIDE:   P13518
- EnsemblBacteria:   EBESCT00000004605
- EnsemblBacteria:   EBESCT00000016596
- GeneID:   947702
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3221
- KEGG:   eco:b3252
- EchoBASE:   EB0018
- EcoGene:   EG10018
- eggNOG:   COG2200
- GeneTree:   EBGT00050000008778
- HOGENOM:   HBG677930
- OMA:   WFMYDKG
- ProtClustDB:   PRK11059
- BioCyc:   EcoCyc:EG10018-MONOMER
- Genevestigator:   P13518
- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- SMART:   SM00052
- SMART:   SM00267
- TIGRFAMs:   TIGR00254

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; SSF55073 A/G_cyclase

EC number: NA

Molecular weight: Translated: 73340; Mature: 73340

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS50883 EAL; PS50887 GGDEF; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x17a6dc20)-; HASH(0x1773ad88)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQAVATAIDTHLVSNDFSV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSDLFRELSVPLIKHPGMSLR
HHHHHHHHHHHHHHHEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHCCCHHCCCCCEEE
LVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQRQLAGQELLETRATRILNGER
EEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDTLIRSYAAQDVKTGLNNRLFFDN
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECH
QLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHSQVEEQFFTLTNLLSTFMMRYPGAL
HHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHH
LARYHRSDFAALLPHRTLKEAESIAGQLIKAVDTLPNNKMLDRDDMIHIGICAWRSGQDT
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCH
EQVMEHAESATRNAGLQGGNSWAIYDDSLPEKGRGNVRWRTLIEQMLSRGGPRLYQKPAV
HHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
TREGQVHHRELMCRIFDGNEEVSSAEYMPMVLQFGLSEEYDRLQISRLIPLLRYWPEENL
CCCCCHHHHHHHHHEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
AIQVTVESLIRPRFQRWLRDTLMQCEKSQRKRIIIELAEADVGQHISRLQPVIRLVNALG
EEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHC
VRVAVNQAGLTLVSTSWIKELNVELLKLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQV
CEEEECCCCCEEHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
YATGVRSRSEWQTLIQRGVTGGQGDFFASSQPLDTNVKKYSQRYSV
EEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCH
>Mature Secondary Structure
MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQAVATAIDTHLVSNDFSV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSDLFRELSVPLIKHPGMSLR
HHHHHHHHHHHHHHHEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHCCCHHCCCCCEEE
LVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQRQLAGQELLETRATRILNGER
EEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDTLIRSYAAQDVKTGLNNRLFFDN
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECH
QLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHSQVEEQFFTLTNLLSTFMMRYPGAL
HHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHH
LARYHRSDFAALLPHRTLKEAESIAGQLIKAVDTLPNNKMLDRDDMIHIGICAWRSGQDT
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCH
EQVMEHAESATRNAGLQGGNSWAIYDDSLPEKGRGNVRWRTLIEQMLSRGGPRLYQKPAV
HHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
TREGQVHHRELMCRIFDGNEEVSSAEYMPMVLQFGLSEEYDRLQISRLIPLLRYWPEENL
CCCCCHHHHHHHHHEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
AIQVTVESLIRPRFQRWLRDTLMQCEKSQRKRIIIELAEADVGQHISRLQPVIRLVNALG
EEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHC
VRVAVNQAGLTLVSTSWIKELNVELLKLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQV
CEEEECCCCCEEHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
YATGVRSRSEWQTLIQRGVTGGQGDFFASSQPLDTNVKKYSQRYSV
EEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 3049542