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The map label for this gene is ispB [H]

Identifier: 157162671

GI number: 157162671

Start: 3384636

End: 3385607

Strand: Direct

Name: ispB [H]

Synonym: EcHS_A3380

Alternate gene names: 157162671

Gene position: 3384636-3385607 (Clockwise)

Preceding gene: 157162666

Following gene: 157162672

Centisome position: 72.89

GC content: 52.37

Gene sequence:

>972_bases
ATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGTTAATGCGGCAATCCTTGAGCAGCTTAATTC
CGACGTCCAACTGATCAATCAGTTAGGCTATTACATCGTCAGCGGCGGCGGTAAACGTATTCGTCCGATGATTGCTGTAC
TGGCTGCACGAGCTGTTGGCTATGAGGGAAATGCGCATGTCACCATTGCTGCCTTGATCGAGTTTATCCACACGGCGACT
CTGCTACACGACGACGTTGTGGATGAATCAGATATGCGCAGGGGTAAAGCTACCGCCAACGCCGCATTTGGCAATGCCGC
CAGCGTGCTGGTAGGCGATTTTATTTATACCCGCGCTTTCCAGATGATGACCAGCCTCGGTTCACTCAAAGTGCTGGAAG
TCATATCAGAAGCCGTAAACGTCATCGCAGAAGGTGAAGTTCTGCAACTGATGAATGTTAACGATCCGGACATCACTGAA
GAAAACTACATGCGCGTTATCTATAGCAAAACCGCGCGTCTGTTTGAGGCTGCCGCGCAGTGTTCCGGAATTCTGGCTGG
CTGTACGCCGGAGGAGGAGAAAGGCCTGCAGGATTATGGGCGCTATCTCGGCACTGCTTTCCAGTTGATCGACGATTTAC
TCGATTACAATGCCGATGGCGAACAGTTAGGTAAAAACGTCGGCGACGATCTGAACGAAGGTAAACCGACGCTGCCGCTG
CTGCATGCGATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCT
TCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGCGCCGAGGAAGAAGCAGACA
AAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAA
CGCGATCGTTAA

Upstream 100 bases:

>100_bases
TGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGG
CGAAAAGCCCGGCTTTTGCG

Downstream 100 bases:

>100_bases
TCCCCTCCCCTCATCCCGCGCAGTGCGCGGGATGATTCCAGTAAACTCCATAAGAATACTTATTCAGCGTAATAATGCAC
TGAAAAACCAGTGTATACAT

Product: octaprenyl diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGGKRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVISEAVNVIAEGEVLQLMNVNDPDITE
ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNADGEQLGKNVGDDLNEGKPTLPL
LHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMNACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQ
RDR

Sequences:

>Translated_323_residues
MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGGKRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVISEAVNVIAEGEVLQLMNVNDPDITE
ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNADGEQLGKNVGDDLNEGKPTLPL
LHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMNACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQ
RDR
>Mature_323_residues
MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGGKRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVISEAVNVIAEGEVLQLMNVNDPDITE
ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNADGEQLGKNVGDDLNEGKPTLPL
LHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMNACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQ
RDR

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=323, Percent_Identity=30.6501547987616, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI4758430, Length=278, Percent_Identity=23.021582733813, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI83700220, Length=278, Percent_Identity=23.021582733813, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1789578, Length=323, Percent_Identity=99.6904024767802, Blast_Score=660, Evalue=0.0,
Organism=Escherichia coli, GI1786623, Length=254, Percent_Identity=29.9212598425197, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17505681, Length=351, Percent_Identity=33.048433048433, Blast_Score=152, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6319475, Length=300, Percent_Identity=33.3333333333333, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6325188, Length=227, Percent_Identity=25.1101321585903, Blast_Score=82, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24651612, Length=298, Percent_Identity=31.5436241610738, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI281365769, Length=282, Percent_Identity=21.6312056737589, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24660002, Length=282, Percent_Identity=21.6312056737589, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35199; Mature: 35199

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGGKRIRPMIAVLAARAVG
CCHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHC
YEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAF
CCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHH
QMMTSLGSLKVLEVISEAVNVIAEGEVLQLMNVNDPDITEENYMRVIYSKTARLFEAAAQ
HHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
CSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNADGEQLGKNVGDDLNEGKPTLPL
HCCHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCHHH
LHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMNACGSLEWTRQRAEEEADKAIAALQ
HHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VLPDTPWREALIGLAHIAVQRDR
HCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGGKRIRPMIAVLAARAVG
CCHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHC
YEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAF
CCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHH
QMMTSLGSLKVLEVISEAVNVIAEGEVLQLMNVNDPDITEENYMRVIYSKTARLFEAAAQ
HHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
CSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNADGEQLGKNVGDDLNEGKPTLPL
HCCHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCHHH
LHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMNACGSLEWTRQRAEEEADKAIAALQ
HHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VLPDTPWREALIGLAHIAVQRDR
HCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]