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The map label for this gene is glcF

Identifier: 157162450

GI number: 157162450

Start: 3161870

End: 3163093

Strand: Reverse

Name: glcF

Synonym: EcHS_A3150

Alternate gene names: 157162450

Gene position: 3163093-3161870 (Counterclockwise)

Preceding gene: 157162451

Following gene: 157162449

Centisome position: 68.12

GC content: 55.64

Gene sequence:

>1224_bases
ATGCAAACCCAATTAACTGAAGAGATGCGGCAGAACGCGCGCGCGCTGGAAGCCGACAGCATCCTGCGCGCCTGTGTTCA
CTGCGGATTTTGTACCGCAACCTGCCCAACCTATCAGCTTCTGGGCGATGAACTGGACGGGCCGCGCGGGCGCATCTATC
TGATTAAACAGGTGCTGGAAGGCAACGAAGTCACGCTTAAAACACAGGAGCATCTCGATCGCTGCCTCACTTGCCGTAAT
TGTGAAACCACCTGTCCTTCTGGTGTGCGCTATCACAATTTGCTGGATATCGGGCGTGATATTGTCGAGCAGAAAGTGAA
ACGCCCACTGCCGGAGCGAATACTGCGCGAAGGATTGCGCCAGGTAGTGCCGCGTCCGGCGGTCTTCCGTGCGCTGACGC
AGGTAGGGCTGGTGCTGCGACCGTTTTTACCGGAACAGGTCAGAGCAAAACTGCCTGCTGAAACGGTGAAAGCTAAACCG
CGTCCGCCGCTGCGCCATAAGCGTCGGGTTTTAATGTTGGAAGGCTGCGCCCAGCCTACGCTTTCGCCCAACACCAACGC
GGCAACTGCGCGAGTGCTGGATCGTCTGGGGATCAGCGTCATGCCAGCTAACGAAGCAGGCTGTTGTGGCGCGGTGGACT
ATCATCTTAATGCGCAGGAGAAAGGGCTGGCACGGGCGCGCAATAATATTGATGCCTGGTGGCCCGCGATTGAAGCAGGT
GCCGAGGCAATTTTGCAAACCGCCAGCGGCTGCGGCGCGTTTGTCAAAGAGTATGGGCAGATGCTGAAAAACGATGCGTT
ATATGCCGATAAAGCGCGTCAGGTCAGTGAACTGGCGGTCGATTTAGTCGAACTTCTGCGCGAGGAACCGCTGGAAAAAC
TGGCAATTCGCGGCGATAAAAAGCTGGCCTTCCACTGTCCGTGTACCCTACAACATGCGCAAAAGCTGAACGGCGAAGTG
GAAAAAGTGTTGCTTCGTCTTGGATTTACCTTAACGGACGTTCCCGACAGCCATCTGTGCTGCGGTTCAGCGGGAACATA
TGCGTTAACGCATCCCGATCTGGCACGCCAGCTGCGGGATAACAAAATGAATGCGCTGGAAAGCGGCAAACCGGAAATGA
TCGTCACCGCCAACATTGGTTGCCAGACGCATCTGGCGAGCGCCGGTCGTACCTCTGTGCGTCACTGGATTGAAATTGTA
GAACAAGCCCTTGAAAAGGAATAA

Upstream 100 bases:

>100_bases
GCTCCTTTATTCCGCTATCACCAGCAGCTTAAACAGCAGCTCGACCCTTGCGGCGTGTTTAACCCCGGTCGCATGTACGC
GGAACTTTGAGGAGCAGGCT

Downstream 100 bases:

>100_bases
CAAAATGAAAACTAAAGTCATTCTTAGCCAGCAAATGGCGAGTGCAATTATTGCCGCAGGTCAGGAAGAGGCGCAGAAAA
ATAACTGGTCTGTTTCCATT

Product: glycolate oxidase iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRN
CETTCPSGVRYHNLLDIGRDIVEQKVKRPLPERILREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNAQEKGLARARNNIDAWWPAIEAG
AEAILQTASGCGAFVKEYGQMLKNDALYADKARQVSELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEV
EKVLLRLGFTLTDVPDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTSVRHWIEIV
EQALEKE

Sequences:

>Translated_407_residues
MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRN
CETTCPSGVRYHNLLDIGRDIVEQKVKRPLPERILREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNAQEKGLARARNNIDAWWPAIEAG
AEAILQTASGCGAFVKEYGQMLKNDALYADKARQVSELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEV
EKVLLRLGFTLTDVPDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTSVRHWIEIV
EQALEKE
>Mature_407_residues
MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRN
CETTCPSGVRYHNLLDIGRDIVEQKVKRPLPERILREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNAQEKGLARARNNIDAWWPAIEAG
AEAILQTASGCGAFVKEYGQMLKNDALYADKARQVSELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEV
EKVLLRLGFTLTDVPDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTSVRHWIEIV
EQALEKE

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI48994913, Length=407, Percent_Identity=100, Blast_Score=835, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLCF_ECOLI (P52074)

Other databases:

- EMBL:   L43490
- EMBL:   U28377
- EMBL:   U00096
- EMBL:   AP009048
- RefSeq:   AP_003532.1
- RefSeq:   YP_026190.1
- ProteinModelPortal:   P52074
- SMR:   P52074
- STRING:   P52074
- EnsemblBacteria:   EBESCT00000001800
- EnsemblBacteria:   EBESCT00000014289
- GeneID:   2847717
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5486
- KEGG:   eco:b4467
- EchoBASE:   EB3076
- EcoGene:   EG13291
- eggNOG:   COG0247
- GeneTree:   EBGT00050000011307
- HOGENOM:   HBG646784
- OMA:   DYMHLVD
- ProtClustDB:   PRK11274
- BioCyc:   EcoCyc:MONOMER0-561
- BioCyc:   MetaCyc:MONOMER0-561
- Genevestigator:   P52074
- GO:   GO:0006810
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257
- Gene3D:   G3DSA:1.10.1060.10
- PIRSF:   PIRSF000139

Pfam domain/function: PF02754 CCG

EC number: NA

Molecular weight: Translated: 45111; Mature: 45111

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLE
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHCCCCCCCCCHHHHHHHHHC
GNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIVEQKVKRPLPERILREGLR
CCCEEEEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
QVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKPRPPLRHKRRVLMLEGCAQPT
HHCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHCCCCCCCHHHHCCEEEEECCCCCC
LSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNAQEKGLARARNNIDAWWPAIEAG
CCCCCCHHHHHHHHHHCCEEECCCCCCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHHHH
AEAILQTASGCGAFVKEYGQMLKNDALYADKARQVSELAVDLVELLREEPLEKLAIRGDK
HHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCC
KLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDVPDSHLCCGSAGTYALTHPDLARQLRD
CEEEECCCHHHHHHHHCCHHHHHHHHHCCCEECCCCCCEEECCCCCEEEECHHHHHHHHH
NKMNALESGKPEMIVTANIGCQTHLASAGRTSVRHWIEIVEQALEKE
HHHHHHHCCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLE
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHCCCCCCCCCHHHHHHHHHC
GNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIVEQKVKRPLPERILREGLR
CCCEEEEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
QVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKPRPPLRHKRRVLMLEGCAQPT
HHCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHCCCCCCCHHHHCCEEEEECCCCCC
LSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNAQEKGLARARNNIDAWWPAIEAG
CCCCCCHHHHHHHHHHCCEEECCCCCCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHHHH
AEAILQTASGCGAFVKEYGQMLKNDALYADKARQVSELAVDLVELLREEPLEKLAIRGDK
HHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCC
KLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDVPDSHLCCGSAGTYALTHPDLARQLRD
CEEEECCCHHHHHHHHCCHHHHHHHHHCCCEECCCCCCEEECCCCCEEEECHHHHHHHHH
NKMNALESGKPEMIVTANIGCQTHLASAGRTSVRHWIEIVEQALEKE
HHHHHHHCCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503