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The map label for this gene is gspF2 [H]

Identifier: 157162436

GI number: 157162436

Start: 3144205

End: 3145428

Strand: Reverse

Name: gspF2 [H]

Synonym: EcHS_A3136

Alternate gene names: 157162436

Gene position: 3145428-3144205 (Counterclockwise)

Preceding gene: 157162437

Following gene: 157162435

Centisome position: 67.74

GC content: 58.42

Gene sequence:

>1224_bases
ATGGCACTGTTTTACTATCAGGCGCTGGAGCGTAATGGTCGCAAAACCAAAGGTATGATTGAGGCGGATTCCGCGCGTCA
TGCCCGCCAGTTGTTGCGCGGTAAAGAGCTTATCCCCGTGCATATTGAAGCCCGGATGAATACTTCGTCAGGGGGGATGT
TGCAGCGTCGGCGGCACGCACATCGTCGCGTGGCGGCGGCAGATCTTGCGCTGTTCACGCGCCAACTGGCAACGCTGGTA
CAGGCAGCAATGCCGCTGGAAACCTGCTTACAGGCGGTCAGTGAGCAAAGTGAAAAACTGCATGTAAAAAGCCTCGGAAT
GGCGCTGCGCAGCCGGATTCAGGAAGGTTACACCCTGTCGGACAGCCTGCGCGAACATCCCCGCGTCTTTGATTCTCTGT
TTTGTTCGATGGTTGCTGCCGGAGAAAAATCCGGGCATCTCGACGTGGTGCTCAATCGCCTGGCAGATTACACCGAACAG
CGACAGCGCCTGAAATCACGTCTATTGCAGGCCATGCTCTATCCGCTGGTTCTGCTGGTGGTGGCAACGGGCGTAGTCAC
TATTTTGCTGACGGCAGTGGTGCCGAAAATCATCGAACAGTTCGATCACCTCGGACACGCGCTGCCCGCCACCACCCGCG
CGCTTATCGCCATGAGCGACGCGTTACAGGCCAGCGGCGTTTACTGGCTGGCGGGATTGCTGGCGCTTCTGGTGCTGGGG
CAACGGCTACTTAAAAATCCTGCTATGCGCCTGCGCCGGGATAAAACCTTGCTGCGTCTGCCCGTGACGGGCCGTGTTGC
GCGCGGGCTGAATACGGCGCGTTTTTCCCGCACATTAAGCATCCTCACCGCCAGCAGTGTTCCGCTACTGGAAGGCATTC
AGACCGCTGCCGCCGTGTCGGCAAATCGCTATGTCGAACAACAACTACTGCTGGCGGCAGATCGCGTCCGCGAAGGAAGC
AGTCTGCGTGCCGCGCTGGCGGAGTTGCGCCTGTTCCCCCCGATGATGCTGTACATGATCGCCTCCGGCGAACAGAGCGG
CGAACTGGAAACCATGCTTGAGCAGGCCGCTGTTAACCAGGAACGGGAATTTGATACCCAGGTGGGGCTGGCGTTAGGGC
TGTTTGAACCGGCGCTGGTGGTGATGATGGCGGGCGTGGTGCTGTTTATCGTCATCGCCATCCTCGAGCCGATGCTGCAA
CTGAACAATATGGTTGGAATGTAA

Upstream 100 bases:

>100_bases
TCCGACAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTGCTCGCCGGAGAAACCTCTCTCGATGAAGTTCTG
CGGGTAACCATGGAGGCGTA

Downstream 100 bases:

>100_bases
TTTACGGAGTTATCACATGAATTCGTTATCCCGCACACAAAAACCACGGGCAGGTTTTACCCTGCTGGAAGTGATGGTGG
TGATTGTTATTCTTGGCGTC

Product: general secretion pathway protein F

Products: NA

Alternate protein names: Cholera toxin secretion protein epsF [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEARMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLV
QAAMPLETCLQAVSEQSEKLHVKSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPATTRALIAMSDALQASGVYWLAGLLALLVLG
QRLLKNPAMRLRRDKTLLRLPVTGRVARGLNTARFSRTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGS
SLRAALAELRLFPPMMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVIAILEPMLQ
LNNMVGM

Sequences:

>Translated_407_residues
MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEARMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLV
QAAMPLETCLQAVSEQSEKLHVKSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPATTRALIAMSDALQASGVYWLAGLLALLVLG
QRLLKNPAMRLRRDKTLLRLPVTGRVARGLNTARFSRTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGS
SLRAALAELRLFPPMMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVIAILEPMLQ
LNNMVGM
>Mature_406_residues
ALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEARMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQ
AAMPLETCLQAVSEQSEKLHVKSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQR
QRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPATTRALIAMSDALQASGVYWLAGLLALLVLGQ
RLLKNPAMRLRRDKTLLRLPVTGRVARGLNTARFSRTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSS
LRAALAELRLFPPMMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVIAILEPMLQL
NNMVGM

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=403, Percent_Identity=45.409429280397, Blast_Score=329, Evalue=2e-91,
Organism=Escherichia coli, GI1786295, Length=409, Percent_Identity=25.6723716381418, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 44893; Mature: 44761

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEARMNTSSGGMLQRRRHA
CCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHHHH
HRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHVKSLGMALRSRIQEGYTLS
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
DSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQRQRLKSRLLQAMLYPLVLLV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VATGVVTILLTAVVPKIIEQFDHLGHALPATTRALIAMSDALQASGVYWLAGLLALLVLG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
QRLLKNPAMRLRRDKTLLRLPVTGRVARGLNTARFSRTLSILTASSVPLLEGIQTAAAVS
HHHHHCHHHHHHHCCCEEECCCCHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ANRYVEQQLLLAADRVREGSSLRAALAELRLFPPMMLYMIASGEQSGELETMLEQAAVNQ
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHCC
EREFDTQVGLALGLFEPALVVMMAGVVLFIVIAILEPMLQLNNMVGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEARMNTSSGGMLQRRRHA
CHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHHHH
HRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHVKSLGMALRSRIQEGYTLS
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
DSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQRQRLKSRLLQAMLYPLVLLV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VATGVVTILLTAVVPKIIEQFDHLGHALPATTRALIAMSDALQASGVYWLAGLLALLVLG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
QRLLKNPAMRLRRDKTLLRLPVTGRVARGLNTARFSRTLSILTASSVPLLEGIQTAAAVS
HHHHHCHHHHHHHCCCEEECCCCHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ANRYVEQQLLLAADRVREGSSLRAALAELRLFPPMMLYMIASGEQSGELETMLEQAAVNQ
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHCC
EREFDTQVGLALGLFEPALVVMMAGVVLFIVIAILEPMLQLNNMVGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]