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The map label for this gene is speA [H]

Identifier: 157162397

GI number: 157162397

Start: 3111562

End: 3113538

Strand: Reverse

Name: speA [H]

Synonym: EcHS_A3096

Alternate gene names: 157162397

Gene position: 3113538-3111562 (Counterclockwise)

Preceding gene: 157162398

Following gene: 157162396

Centisome position: 67.05

GC content: 54.32

Gene sequence:

>1977_bases
ATGTCTGACGACATGTCTATGGGTTTGCCTTCGTCAGCGGGCGAACACGGTGTACTACGCTCCATGCAGGAGGTTGCAAT
GAGCTCCCAGGAAGCCAGCAAGATGCTGCGTACTTACAATATTGCCTGGTGGGGCAATAACTACTATGACGTTAACGAGC
TGGGCCACATCAGCGTGTGCCCGGACCCGGACGTCCCGGAAGCTCGCGTCGATCTCGCGCAGTTAGTGAAAACTCGTGAA
GCACAGGGTCAGCGTCTGCCTGCACTGTTCTGTTTCCCACAGATCCTGCAGCACCGTTTGCGTTCCATTAACGCCGCGTT
CAAACGTGCGCGGGAATCCTACGGCTATAACGGCGATTACTTCCTTGTTTATCCGATCAAAGTTAACCAGCACCGCCGCG
TGATTGAGTCCCTGATTCATTCGGGCGAACCGCTGGGACTGGAAGCAGGCTCGAAAGCAGAGTTGATGGCGGTGCTGGCA
CATGCTGGCATGACCCGTAGCGTCATCGTCTGCAACGGTTATAAAGACCGCGAATATATCCGCCTGGCATTAATTGGCGA
GAAGATGGGCCACAAGGTCTACCTGGTCATTGAGAAGATGTCAGAAATCGCCATTGTGCTGGATGAAGCAGAACGTCTGA
ATGTCGTTCCTCGTCTGGGCGTGCGTGCACGTCTGGCTTCGCAGGGCTCGGGTAAATGGCAGTCCTCCGGCGGGGAAAAA
TCGAAGTTCGGCCTGGCTGCGACTCAGGTACTGCAACTGGTTGAAACCCTGCGTGAAGCCGGGCGTCTCGACAGCCTGCA
ACTACTGCACTTCCACCTCGGTTCGCAGATGGCGAATATTCGCGATATCGCCACAGGCGTTCGTGAATCGGCGCGTTTCT
ATGTGGAACTGCACAAGCTGGGCGTCAATATTCAGTGCTTCGACGTCGGCGGCGGTCTGGGCGTGGACTATGAAGGCACC
CGTTCGCAGTCTGACTGCTCCGTAAACTACGGCCTCAATGAATATGCCAACAACATCATCTGGGCGATTGGTGATGCGTG
TGAAGAAAACGGTCTGCCGCATCCGACGGTAATCACCGAATCGGGTCGTGCGGTGACTGCGCATCACACCGTGCTGGTGT
CTAATATCATCGGCGTGGAACGTAACGAATACACGGTGCCGACCGCGCCTGCAGAAGATGCGCCGCGCGCGCTGCAAAGC
ATGTGGGAAACCTGGCAGGAGATGCACGAACCGGGAACTCGCCGTTCTCTGCGTGAATGGTTACACGACAGTCAGATGGA
TCTGCACGACATTCATATCGGCTACTCTTCCGGCACCTTTAGCCTGCAAGAACGTGCATGGGCAGAACAACTCTATCTGA
GCATGTGCCATGAAGTGCAGAAGCAACTGGATCCGCAAAACCGTGCGCATCGTCCGATAATCGACGAACTGCAGGAACGT
ATGGCGGACAAAATGTACGTCAATTTCTCGCTGTTCCAGTCAATGCCGGACGCATGGGGGATCGACCAGTTGTTCCCGGT
TCTGCCGCTGGAAGGGCTGGATCAAGTGCCGGAACGTCGCGCTGTGCTGCTGGATATTACCTGTGACTCTGACGGTGCTA
TCGACCACTATATTGATGGTGATGGTATTGCCACGACAATGCCAATGCCGGAGTACGATCCAGAGAATCCGCCGATGCTC
GGTTTCTTTATGGTCGGCGCATATCAGGAGATCCTTGGCAATATGCACAACCTGTTCGGTGATACCGAAGCGGTTGACGT
GTTCGTGTTCCCTGACGGTAGCGTAGAAGTAGAACTGTCTGACGAAGGCGATACCGTGGCGGACATGCTGCAATATGTAC
AGCTCGATCCGAAAACGCTGTTAACCCAGTTCCGCGATCAAGTGAAGAAAACCGATCTTGATGCTGAACTGCAACAACAG
TTCCTTGAAGAGTTCGAGGCAGGTTTGTACGGTTATACTTATCTTGAAGATGAGTAA

Upstream 100 bases:

>100_bases
CACTGCTGGAAAATCCATGTGCTTATGGGTTGTTATCGCAGTTCCAGGCTGCGATAGTCGTTAACTGTTTTACACTTAAT
AAAATAATTTGAGGTTCGCT

Downstream 100 bases:

>100_bases
GTCCTGTGTTACTTGAATCCGCTTAATTTAGCGGTGATAATCCGCCACAATTTATTGTGACAAATCCAACCCTTCCTCGT
CGGGCCTAACGACGCGGAAG

Product: arginine decarboxylase

Products: NA

Alternate protein names: ADC [H]

Number of amino acids: Translated: 658; Mature: 657

Protein sequence:

>658_residues
MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWWGNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTRE
AQGQRLPALFCFPQILQHRLRSINAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPRLGVRARLASQGSGKWQSSGGEK
SKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQMANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGT
RSQSDCSVNYGLNEYANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAEDAPRALQS
MWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQLYLSMCHEVQKQLDPQNRAHRPIIDELQER
MADKMYVNFSLFQSMPDAWGIDQLFPVLPLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPML
GFFMVGAYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQFRDQVKKTDLDAELQQQ
FLEEFEAGLYGYTYLEDE

Sequences:

>Translated_658_residues
MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWWGNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTRE
AQGQRLPALFCFPQILQHRLRSINAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPRLGVRARLASQGSGKWQSSGGEK
SKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQMANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGT
RSQSDCSVNYGLNEYANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAEDAPRALQS
MWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQLYLSMCHEVQKQLDPQNRAHRPIIDELQER
MADKMYVNFSLFQSMPDAWGIDQLFPVLPLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPML
GFFMVGAYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQFRDQVKKTDLDAELQQQ
FLEEFEAGLYGYTYLEDE
>Mature_657_residues
SDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWWGNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREA
QGQRLPALFCFPQILQHRLRSINAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLAH
AGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPRLGVRARLASQGSGKWQSSGGEKS
KFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQMANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTR
SQSDCSVNYGLNEYANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAEDAPRALQSM
WETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQLYLSMCHEVQKQLDPQNRAHRPIIDELQERM
ADKMYVNFSLFQSMPDAWGIDQLFPVLPLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLG
FFMVGAYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQFRDQVKKTDLDAELQQQF
LEEFEAGLYGYTYLEDE

Specific function: Catalyzes the biosynthesis of agmatine from arginine [H]

COG id: COG1166

COG function: function code E; Arginine decarboxylase (spermidine biosynthesis)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Orn/Lys/Arg decarboxylase class-II family. SpeA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789307, Length=658, Percent_Identity=99.8480243161094, Blast_Score=1367, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009006
- InterPro:   IPR002985
- InterPro:   IPR022643
- InterPro:   IPR022657
- InterPro:   IPR022644
- InterPro:   IPR022653
- InterPro:   IPR000183 [H]

Pfam domain/function: PF02784 Orn_Arg_deC_N; PF00278 Orn_DAP_Arg_deC [H]

EC number: =4.1.1.19 [H]

Molecular weight: Translated: 73887; Mature: 73756

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00878 ODR_DC_2_1 ; PS00879 ODR_DC_2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWWGNNYYDVNELGHISVC
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEECCCCCEEEEC
PDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRSINAAFKRARESYGYNGDY
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
FLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLAHAGMTRSVIVCNGYKDREYI
EEEEEEEECHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEE
RLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPRLGVRARLASQGSGKWQSSGGEK
EEEEEHHHHCCEEEEEEEHHCCCEEEECCHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCC
SKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQMANIRDIATGVRESARFYVELHKL
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHH
GVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNEYANNIIWAIGDACEENGLPHPTVITE
CCEEEEEECCCCCCCCCCCCCCCCCCEEECCHHHHHCCEEEEECCHHHHCCCCCCEEEEC
SGRAVTAHHTVLVSNIIGVERNEYTVPTAPAEDAPRALQSMWETWQEMHEPGTRRSLREW
CCCEEEHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LHDSQMDLHDIHIGYSSGTFSLQERAWAEQLYLSMCHEVQKQLDPQNRAHRPIIDELQER
HCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
MADKMYVNFSLFQSMPDAWGIDQLFPVLPLEGLDQVPERRAVLLDITCDSDGAIDHYIDG
HHHHHEEEHHHHHCCCCCCCHHHHHHCCCCCCHHCCCCCCEEEEEEEECCCCCEEEEECC
DGIATTMPMPEYDPENPPMLGFFMVGAYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELS
CCCEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEC
DEGDTVADMLQYVQLDPKTLLTQFRDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCC
>Mature Secondary Structure 
SDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWWGNNYYDVNELGHISVC
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEECCCCCEEEEC
PDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRSINAAFKRARESYGYNGDY
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
FLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLAHAGMTRSVIVCNGYKDREYI
EEEEEEEECHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEE
RLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPRLGVRARLASQGSGKWQSSGGEK
EEEEEHHHHCCEEEEEEEHHCCCEEEECCHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCC
SKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQMANIRDIATGVRESARFYVELHKL
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHH
GVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNEYANNIIWAIGDACEENGLPHPTVITE
CCEEEEEECCCCCCCCCCCCCCCCCCEEECCHHHHHCCEEEEECCHHHHCCCCCCEEEEC
SGRAVTAHHTVLVSNIIGVERNEYTVPTAPAEDAPRALQSMWETWQEMHEPGTRRSLREW
CCCEEEHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LHDSQMDLHDIHIGYSSGTFSLQERAWAEQLYLSMCHEVQKQLDPQNRAHRPIIDELQER
HCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
MADKMYVNFSLFQSMPDAWGIDQLFPVLPLEGLDQVPERRAVLLDITCDSDGAIDHYIDG
HHHHHEEEHHHHHCCCCCCCHHHHHHCCCCCCHHCCCCCCEEEEEEEECCCCCEEEEECC
DGIATTMPMPEYDPENPPMLGFFMVGAYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELS
CCCEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEC
DEGDTVADMLQYVQLDPKTLLTQFRDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12384590; 12704152 [H]