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The map label for this gene is speB [H]

Identifier: 157162396

GI number: 157162396

Start: 3110504

End: 3111424

Strand: Reverse

Name: speB [H]

Synonym: EcHS_A3095

Alternate gene names: 157162396

Gene position: 3111424-3110504 (Counterclockwise)

Preceding gene: 157162397

Following gene: 157162394

Centisome position: 67.01

GC content: 56.46

Gene sequence:

>921_bases
ATGAGCACCTTAGGTCATCAATACGATAACTCACTGGTTTCCAACGCCTTTGGTTTTTTACGCCTGCCGATGAACTTCCA
GCCGTATGACAGCGATGCCGACTGGGTGATCACCGGCGTGCCGTTCGACATGGCCACCTCTGGCCGTGCGGGTGGTCGCC
ACGGTCCGGCAGCGATCCGTCAGGTTTCGACGAATCTGGCCTGGGAACACAACCGCTTCCCGTGGAATTTCGACATGCGT
GAGCGTCTGAACGTCGTGGACTGCGGCGATCTGGTATATGCCTTTGGCGATGCCCGTGAGATGAGCGAGAAGCTGCAGGC
GCACGCCGAGAAGCTGCTGGCTGCCGGTAAGCGTATGCTCTCCTTCGGTGGTGACCACTTTGTTACGCTGCCGCTGCTGC
GTGCTCATGCGAAGCATTTCGGCAAAATGGCGCTGGTACACTTTGACGCCCACACCGATACCTACGCGAACGGTTGTGAA
TTTGACCACGGCACCATGTTCTATACCGCGCCGAAAGAAGGTCTGATCGACCCGAATCATTCCGTGCAGATTGGTATTCG
TACCGAGTTTGATAAAGACAACGGTTTTACCGTGCTGGACGCCTGCCAGGTGAACGATCGCAGCGTGGATGACGTTATCG
CCCAAGTGAAACAGATTGTGGGTGATATGTCGGTTTACCTGACCTTTGATATCGACTGCCTGGATCCTGCTTTCGCGCCG
GGAACCGGTACGCCAGTGATTGGCGGCCTGACCTCCGATCGCGCCATTAAACTGGTACGCGGCCTGAAAGATCTCAACAT
CGTTGGGATGGACGTAGTGGAAGTGGCTCCGGCATACGATCAGTCGGAAATCACCGCTCTGGCTGCGGCGACGCTGGCGC
TGGAGATGCTCTATATTCAGGCGGCGAAAAAGGGCGAGTAA

Upstream 100 bases:

>100_bases
TAATCCGCCACAATTTATTGTGACAAATCCAACCCTTCCTCGTCGGGCCTAACGACGCGGAAGGGTTTTTTTATATCGAC
TTTGTAATAGGAGTCCATCC

Downstream 100 bases:

>100_bases
GCACCAGATGCGATGCGCACGGGTAAAACGTGCCATTAATGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGTTCGG
CACCCGTAGGCCGGATAAGA

Product: agmatinase

Products: NA

Alternate protein names: Agmatine ureohydrolase; AUH [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDMATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMR
ERLNVVDCGDLVYAFGDAREMSEKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQIVGDMSVYLTFDIDCLDPAFAP
GTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVAPAYDQSEITALAAATLALEMLYIQAAKKGE

Sequences:

>Translated_306_residues
MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDMATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMR
ERLNVVDCGDLVYAFGDAREMSEKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQIVGDMSVYLTFDIDCLDPAFAP
GTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVAPAYDQSEITALAAATLALEMLYIQAAKKGE
>Mature_305_residues
STLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDMATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRE
RLNVVDCGDLVYAFGDAREMSEKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCEF
DHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQIVGDMSVYLTFDIDCLDPAFAPG
TGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVAPAYDQSEITALAAATLALEMLYIQAAKKGE

Specific function: Catalyzes the formation of putrescine from agmatine [H]

COG id: COG0010

COG function: function code E; Arginase/agmatinase/formimionoglutamate hydrolase, arginase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the arginase family. Agmatinase subfamily [H]

Homologues:

Organism=Homo sapiens, GI37537722, Length=292, Percent_Identity=40.7534246575342, Blast_Score=202, Evalue=4e-52,
Organism=Escherichia coli, GI1789306, Length=306, Percent_Identity=99.6732026143791, Blast_Score=634, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71996843, Length=294, Percent_Identity=22.108843537415, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005925
- InterPro:   IPR006035
- InterPro:   IPR020855 [H]

Pfam domain/function: PF00491 Arginase [H]

EC number: =3.5.3.11 [H]

Molecular weight: Translated: 33547; Mature: 33416

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00147 ARGINASE_1 ; PS00148 ARGINASE_2 ; PS01053 ARGINASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDMATSGRAGGRHGPAAIR
CCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHH
QVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMSEKLQAHAEKLLAAGKRML
HHHHCCCEECCCCCCCCCHHHCCCEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
SFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCEFDHGTMFYTAPKEGLIDPNH
HCCCCCEEEHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCC
SVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQIVGDMSVYLTFDIDCLDPAFAP
CEEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCC
GTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVAPAYDQSEITALAAATLALEMLYIQ
CCCCCEECCCCCHHHHHHHHCCCCCCEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AAKKGE
HHCCCC
>Mature Secondary Structure 
STLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDMATSGRAGGRHGPAAIR
CCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHH
QVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMSEKLQAHAEKLLAAGKRML
HHHHCCCEECCCCCCCCCHHHCCCEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
SFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCEFDHGTMFYTAPKEGLIDPNH
HCCCCCEEEHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCC
SVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQIVGDMSVYLTFDIDCLDPAFAP
CEEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCC
GTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVAPAYDQSEITALAAATLALEMLYIQ
CCCCCEECCCCCHHHHHHHHCCCCCCEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AAKKGE
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA