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The map label for this gene is ndk [H]

Identifier: 157161993

GI number: 157161993

Start: 2682072

End: 2682503

Strand: Reverse

Name: ndk [H]

Synonym: EcHS_A2669

Alternate gene names: 157161993

Gene position: 2682503-2682072 (Counterclockwise)

Preceding gene: 157161994

Following gene: 157161992

Centisome position: 57.77

GC content: 52.31

Gene sequence:

>432_bases
ATGGCTATTGAACGTACTTTTTCCATCATCAAACCGAACGCGGTAGCAAAAAACGTCATTGGTAATATCTTTGCGCGCTT
TGAAGCTGCAGGGTTCAAAATTGTTGGCACCAAAATGCTGCACCTGACCGTTGAACAGGCACGTGGCTTTTATGCTGAAC
ACGATGGAAAACCGTTCTTTGATGGTCTGGTTGAATTCATGACCTCTGGCCCGATCGTGGTTTCCGTGCTGGAAGGTGAA
AACGCCGTTCAGCGTCACCGCGATCTGCTGGGCGCGACCAATCCGGCAAACGCACTGGCTGGTACTCTGCGCGCTGATTA
CGCTGACAGCCTGACCGAAAACGGTACCCACGGTTCTGATTCCGTCGAATCTGCCGCTCGCGAAATCGCTTATTTCTTTG
GCGAAGGCGAAGTGTGCCCGCGCACCCGTTAA

Upstream 100 bases:

>100_bases
TGTTACCTTCATCAATAGTCAACGGCCCTGTTGCTCATTATAATCCGCGCCATCTCGTACGCTGGTACAGACAACAACAG
AACAATTTACAGAGGTAAAA

Downstream 100 bases:

>100_bases
TAATTTCGTAAATGCCGCGTGCAAACGTGGCATCCGTGCGCCAGAATTTGTACAATGCAGCGCCCCCGGACGAGCAGCCG
CTCACCGGGGCGTTTCTTTT

Product: nucleoside diphosphate kinase

Products: NA

Alternate protein names: NDK; NDP kinase; Nucleoside-2-P kinase [H]

Number of amino acids: Translated: 143; Mature: 142

Protein sequence:

>143_residues
MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTVEQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGE
NAVQRHRDLLGATNPANALAGTLRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR

Sequences:

>Translated_143_residues
MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTVEQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGE
NAVQRHRDLLGATNPANALAGTLRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR
>Mature_142_residues
AIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTVEQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGEN
AVQRHRDLLGATNPANALAGTLRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR

Specific function: Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate [H]

COG id: COG0105

COG function: function code F; Nucleoside diphosphate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NDK family [H]

Homologues:

Organism=Homo sapiens, GI37693993, Length=135, Percent_Identity=46.6666666666667, Blast_Score=129, Evalue=1e-30,
Organism=Homo sapiens, GI66392227, Length=136, Percent_Identity=44.1176470588235, Blast_Score=126, Evalue=5e-30,
Organism=Homo sapiens, GI66392205, Length=136, Percent_Identity=44.1176470588235, Blast_Score=126, Evalue=5e-30,
Organism=Homo sapiens, GI4505409, Length=136, Percent_Identity=44.1176470588235, Blast_Score=126, Evalue=5e-30,
Organism=Homo sapiens, GI66392192, Length=136, Percent_Identity=44.1176470588235, Blast_Score=126, Evalue=5e-30,
Organism=Homo sapiens, GI66392203, Length=136, Percent_Identity=44.1176470588235, Blast_Score=126, Evalue=5e-30,
Organism=Homo sapiens, GI4557797, Length=139, Percent_Identity=43.8848920863309, Blast_Score=125, Evalue=1e-29,
Organism=Homo sapiens, GI38045913, Length=130, Percent_Identity=45.3846153846154, Blast_Score=125, Evalue=1e-29,
Organism=Homo sapiens, GI4826862, Length=136, Percent_Identity=39.7058823529412, Blast_Score=114, Evalue=4e-26,
Organism=Homo sapiens, GI5031951, Length=136, Percent_Identity=40.4411764705882, Blast_Score=93, Evalue=9e-20,
Organism=Homo sapiens, GI37574614, Length=144, Percent_Identity=36.1111111111111, Blast_Score=92, Evalue=2e-19,
Organism=Homo sapiens, GI7019465, Length=144, Percent_Identity=36.1111111111111, Blast_Score=91, Evalue=2e-19,
Organism=Homo sapiens, GI4505413, Length=145, Percent_Identity=38.6206896551724, Blast_Score=87, Evalue=5e-18,
Organism=Homo sapiens, GI30023828, Length=135, Percent_Identity=34.8148148148148, Blast_Score=74, Evalue=3e-14,
Organism=Homo sapiens, GI148839372, Length=137, Percent_Identity=32.8467153284672, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1788866, Length=143, Percent_Identity=100, Blast_Score=293, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI17506807, Length=135, Percent_Identity=42.962962962963, Blast_Score=123, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6322783, Length=136, Percent_Identity=44.8529411764706, Blast_Score=130, Evalue=1e-31,
Organism=Drosophila melanogaster, GI45549037, Length=139, Percent_Identity=46.7625899280576, Blast_Score=136, Evalue=5e-33,
Organism=Drosophila melanogaster, GI18860097, Length=136, Percent_Identity=37.5, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001564
- InterPro:   IPR023005 [H]

Pfam domain/function: PF00334 NDK [H]

EC number: =2.7.4.6 [H]

Molecular weight: Translated: 15464; Mature: 15332

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00469 NDP_KINASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTVEQARGFYAEHDGKPFF
CCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCEEEECHHHHEEEHHHHCCCCCCCCCCHHH
DGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGTLRADYADSLTENGTHGSD
HHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCH
SVESAAREIAYFFGEGEVCPRTR
HHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTVEQARGFYAEHDGKPFF
CCCCCHHHHCCHHHHHHHHHHHHHHHHHCCEEEECHHHHEEEHHHHCCCCCCCCCCHHH
DGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGTLRADYADSLTENGTHGSD
HHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCH
SVESAAREIAYFFGEGEVCPRTR
HHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA