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The map label for this gene is 157161975

Identifier: 157161975

GI number: 157161975

Start: 2663465

End: 2664313

Strand: Reverse

Name: 157161975

Synonym: EcHS_A2651

Alternate gene names: NA

Gene position: 2664313-2663465 (Counterclockwise)

Preceding gene: 157161976

Following gene: 157161972

Centisome position: 57.38

GC content: 40.28

Gene sequence:

>849_bases
ATGACAAACGATAAGGTAAAGTTAAATACAAGAAATGTAGATAAACTCATCGCTATACATAAGTGCAGTGCTCTGGAGGC
TAATGAAATTGGTTTCATTGCTCGGTTTTTAATTAAGGCAACCCTGCCACACAGCAAGCCAAGATTGAATGAGTGGAGCC
GTAAAAATGGTAATTTAACCATGCATATGATGGCACCATCAGCAGTAGGTTTGCCTTACGGTTGTTATGCTCGATTACTA
TTGGTTTGGATTAGTACTCAGGCAATTAGAAATAAATCAAAATTAGACAAAGGTATTATCACTGAACAGGAAGTTCGTAA
GCTTGAATTAGGACGATCTCAGCGACGTTTTATGGAAGCACTGGGGCTACGTTCAAGCGGTGGTAAAAATGGCTCTATTG
TCCCGCTTCGTGAACAGATGCGGCGATTATTCAAAACGACGATTAGTATTATGTTAGCAGAGATAAATGAGGAAGATGGA
TATATTTGTGAAGATGAAATGGGAGCTAGGGTCGCCGATATTTCACATATTTGGTGGAGTGCAAATTATCCTGAACGAAA
TTTATTGAGTCAATCTTGGGTAGAACTTTCGCCTAAATTTTTTCAACTAATAATAGATAAACCTGTTCCGTTGGATATGA
GGGTTCTGCGGCTGATTAAACGTTCTCCGATGGCCTTAGATATATATTGCTGGGCGACTTATCGGGTGAGTTATTTAAAG
CGAGTCACAATTATCCCTTGGTCAGGTTTGATGGAGCAAATGGGATCTAATTATGCCGATGTCAAAGACTTTAAACGACG
GTTTAATGATGGGCTTGAAAAGGTACAGCTGGCATGGCCGGGGCGGTAA

Upstream 100 bases:

>100_bases
AGCAAGACCCGGTTTTATGTGGTCAGTGTGAAGCATCATGACGATTAAAAATGTAATAGATGTTCAGTCTTGGGAAACGA
CTCAGAAAAAAAACGTCTCT

Downstream 100 bases:

>100_bases
TGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAAC
GACAGCGACTTCCGTCCCAG

Product: plasmid encoded RepA protein

Products: NA

Alternate protein names: Plasmid Replication Protein; Replication Protein; Plasmid Replication Initiator RepA; Replicase; RepA Replicase Protein; Replication Protein A; Replicase Protein RepA; RepA Replication Protein; Replication Protein W; Protein Kinase

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTNDKVKLNTRNVDKLIAIHKCSALEANEIGFIARFLIKATLPHSKPRLNEWSRKNGNLTMHMMAPSAVGLPYGCYARLL
LVWISTQAIRNKSKLDKGIITEQEVRKLELGRSQRRFMEALGLRSSGGKNGSIVPLREQMRRLFKTTISIMLAEINEEDG
YICEDEMGARVADISHIWWSANYPERNLLSQSWVELSPKFFQLIIDKPVPLDMRVLRLIKRSPMALDIYCWATYRVSYLK
RVTIIPWSGLMEQMGSNYADVKDFKRRFNDGLEKVQLAWPGR

Sequences:

>Translated_282_residues
MTNDKVKLNTRNVDKLIAIHKCSALEANEIGFIARFLIKATLPHSKPRLNEWSRKNGNLTMHMMAPSAVGLPYGCYARLL
LVWISTQAIRNKSKLDKGIITEQEVRKLELGRSQRRFMEALGLRSSGGKNGSIVPLREQMRRLFKTTISIMLAEINEEDG
YICEDEMGARVADISHIWWSANYPERNLLSQSWVELSPKFFQLIIDKPVPLDMRVLRLIKRSPMALDIYCWATYRVSYLK
RVTIIPWSGLMEQMGSNYADVKDFKRRFNDGLEKVQLAWPGR
>Mature_281_residues
TNDKVKLNTRNVDKLIAIHKCSALEANEIGFIARFLIKATLPHSKPRLNEWSRKNGNLTMHMMAPSAVGLPYGCYARLLL
VWISTQAIRNKSKLDKGIITEQEVRKLELGRSQRRFMEALGLRSSGGKNGSIVPLREQMRRLFKTTISIMLAEINEEDGY
ICEDEMGARVADISHIWWSANYPERNLLSQSWVELSPKFFQLIIDKPVPLDMRVLRLIKRSPMALDIYCWATYRVSYLKR
VTIIPWSGLMEQMGSNYADVKDFKRRFNDGLEKVQLAWPGR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 32533; Mature: 32401

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNDKVKLNTRNVDKLIAIHKCSALEANEIGFIARFLIKATLPHSKPRLNEWSRKNGNLT
CCCCEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEE
MHMMAPSAVGLPYGCYARLLLVWISTQAIRNKSKLDKGIITEQEVRKLELGRSQRRFMEA
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHH
LGLRSSGGKNGSIVPLREQMRRLFKTTISIMLAEINEEDGYICEDEMGARVADISHIWWS
HCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHCCCHHHHHHEEEC
ANYPERNLLSQSWVELSPKFFQLIIDKPVPLDMRVLRLIKRSPMALDIYCWATYRVSYLK
CCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEEHHHHHHHHHH
RVTIIPWSGLMEQMGSNYADVKDFKRRFNDGLEKVQLAWPGR
HEEECCCHHHHHHHCCCCCHHHHHHHHHHCCHHEEEEECCCC
>Mature Secondary Structure 
TNDKVKLNTRNVDKLIAIHKCSALEANEIGFIARFLIKATLPHSKPRLNEWSRKNGNLT
CCCEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEE
MHMMAPSAVGLPYGCYARLLLVWISTQAIRNKSKLDKGIITEQEVRKLELGRSQRRFMEA
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHH
LGLRSSGGKNGSIVPLREQMRRLFKTTISIMLAEINEEDGYICEDEMGARVADISHIWWS
HCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHCCCHHHHHHEEEC
ANYPERNLLSQSWVELSPKFFQLIIDKPVPLDMRVLRLIKRSPMALDIYCWATYRVSYLK
CCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEEHHHHHHHHHH
RVTIIPWSGLMEQMGSNYADVKDFKRRFNDGLEKVQLAWPGR
HEEECCCHHHHHHHCCCCCHHHHHHHHHHCCHHEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA