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The map label for this gene is traJ [H]

Identifier: 157161972

GI number: 157161972

Start: 2661400

End: 2662044

Strand: Reverse

Name: traJ [H]

Synonym: EcHS_A2648

Alternate gene names: 157161972

Gene position: 2662044-2661400 (Counterclockwise)

Preceding gene: 157161975

Following gene: 157161971

Centisome position: 57.33

GC content: 42.17

Gene sequence:

>645_bases
ATGAAATCAGTTATGGCTGAGTTTATAACCACTGAAACGGTGACCGAGGCGCACTATATATCGAATAATTTTACTCGCGG
AATCCTGCATCAATACTCTAAGGAAACACAACATGTACACCTAAATACACATCGACGTGCACAAATATACTACGGCAACT
GGAGGGCAGGACATGTAAAGTGGGCCCACCGCTACGGTGTTCCAACTTTACCACTCCCTATTCGCCGCATCGCGGCTCAA
GGGCGTCCTGCTCTGCGAGGCTGCCAGGCTACCGCCTGTATGGAGAGCTATATGAAAGTTACTCAGACTAGAAAAAATAG
TACGCCAATAAAAGTTTATTGTTTACCCGAAGAAAAAATTCTCATCCAAGAAAATGCAGAAGCATCTGGATTAAGTGTTG
CCTGTTTTGTTCGTAGAGTTGCACTGGGTTATCAGGTTGATTCTATCGTAGATATAAAACAGGTTAATGAACTTAGCCGA
GTAAACGCTGATCTTGGAAGACTTGGCGGATTACTTAAATTATGGCTCGCAAATGATCCTCGAACAGTTGATTTTTCACC
CACACTGATAAGAACCTTGCTTGCGAAAATAGAATCAACTCGGCAAGAACTAAGGAACATTATGGATAAAATCTTGGATA
AATAA

Upstream 100 bases:

>100_bases
TTCTTTTTTTTGCATATAAAACAATTGGTTAGCTGTTGTTGGGGGGCTTTAAGAATTTTAGCCGCTAAAATCCGATAATT
TTTCTTGAGAGTTAGGGGCG

Downstream 100 bases:

>100_bases
ATGTGTGGTTTCCTATATACCTGAGTCTGTTAACAGACAGTGTGTTGAATATTACTGAACTGATGAAAAAGAGGTGAGCA
AATGAATTTAATGTTCCGAG

Product: hypothetical protein

Products: NA

Alternate protein names: Relaxosome protein [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MKSVMAEFITTETVTEAHYISNNFTRGILHQYSKETQHVHLNTHRRAQIYYGNWRAGHVKWAHRYGVPTLPLPIRRIAAQ
GRPALRGCQATACMESYMKVTQTRKNSTPIKVYCLPEEKILIQENAEASGLSVACFVRRVALGYQVDSIVDIKQVNELSR
VNADLGRLGGLLKLWLANDPRTVDFSPTLIRTLLAKIESTRQELRNIMDKILDK

Sequences:

>Translated_214_residues
MKSVMAEFITTETVTEAHYISNNFTRGILHQYSKETQHVHLNTHRRAQIYYGNWRAGHVKWAHRYGVPTLPLPIRRIAAQ
GRPALRGCQATACMESYMKVTQTRKNSTPIKVYCLPEEKILIQENAEASGLSVACFVRRVALGYQVDSIVDIKQVNELSR
VNADLGRLGGLLKLWLANDPRTVDFSPTLIRTLLAKIESTRQELRNIMDKILDK
>Mature_214_residues
MKSVMAEFITTETVTEAHYISNNFTRGILHQYSKETQHVHLNTHRRAQIYYGNWRAGHVKWAHRYGVPTLPLPIRRIAAQ
GRPALRGCQATACMESYMKVTQTRKNSTPIKVYCLPEEKILIQENAEASGLSVACFVRRVALGYQVDSIVDIKQVNELSR
VNADLGRLGGLLKLWLANDPRTVDFSPTLIRTLLAKIESTRQELRNIMDKILDK

Specific function: Transfer of plasmid RP4 during bacterial conjugation requires the plasmid-encoded traJ protein, which binds to a 19- base pair invert sequence repetition within the transfer origin. TraJ protein is bound to only one side of the DNA helix. This nucleoprote

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 24376; Mature: 24376

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSVMAEFITTETVTEAHYISNNFTRGILHQYSKETQHVHLNTHRRAQIYYGNWRAGHVK
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCCEEEEEECCCCCCCEE
WAHRYGVPTLPLPIRRIAAQGRPALRGCQATACMESYMKVTQTRKNSTPIKVYCLPEEKI
EHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCE
LIQENAEASGLSVACFVRRVALGYQVDSIVDIKQVNELSRVNADLGRLGGLLKLWLANDP
EEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCC
RTVDFSPTLIRTLLAKIESTRQELRNIMDKILDK
CEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKSVMAEFITTETVTEAHYISNNFTRGILHQYSKETQHVHLNTHRRAQIYYGNWRAGHVK
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCCEEEEEECCCCCCCEE
WAHRYGVPTLPLPIRRIAAQGRPALRGCQATACMESYMKVTQTRKNSTPIKVYCLPEEKI
EHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCE
LIQENAEASGLSVACFVRRVALGYQVDSIVDIKQVNELSRVNADLGRLGGLLKLWLANDP
EEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCC
RTVDFSPTLIRTLLAKIESTRQELRNIMDKILDK
CEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2663846; 1665997; 2538813; 2168553 [H]