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The map label for this gene is prmB

Identifier: 157161819

GI number: 157161819

Start: 2489079

End: 2490011

Strand: Reverse

Name: prmB

Synonym: EcHS_A2482

Alternate gene names: 157161819

Gene position: 2490011-2489079 (Counterclockwise)

Preceding gene: 157161821

Following gene: 157161818

Centisome position: 53.62

GC content: 53.05

Gene sequence:

>933_bases
GTGGATAAAATTTTCGTTGATGAAGCAGTAAATGAGCTGCAAACCATTCAGGACATGTTGCGCTGGTCGGTGAGCCGCTT
CAGCGCGGCGAATATCTGGTACGGCCACGGCACTGACAACCCGTGGGATGAAGCCGTGCAACTGGTGTTGCCTTCGCTCT
ACCTGCCGTTGGATATCCCGGAAGACATGCGCACCGCGCGTCTGACCTCCAGCGAAAAACACCGTATTGTTGAGCGCGTG
ATTCGCCGCGTCAACGAGCGCATTCCGGTGGCCTACCTGACCAACAAAGCCTGGTTCTGTGGCCATGAATTTTACGTCGA
TGAACGCGTGCTGGTGCCGCGTTCGCCGATTGGTGAACTGATCAACAATAAATTTGCCGGACTTATCAGCAAGCAACCGC
AGCATATTTTAGATATGTGTACTGGTAGCGGCTGCATCGCCATTGCCTGTGCTTATGCCTTCCCGGATGCAGAAGTCGAC
GCGGTGGATATCTCTCCAGACGCGCTGGCGGTTGCTGAACAGAACATCGAAGAACACGGTCTGATCCACAACGTCATTCC
GATTCGTTCCGATCTGTTCCGCGACTTGCCGAAAGTGCAGTACGACCTGATTGTCACTAACCCGCCGTATGTCGATGCGG
AAGATATGTCCGACCTGCCAAACGAATACCGCCACGAGCCGGAACTGGGCCTGGCATCTGGCACTGACGGCCTGAAACTG
ACGCGTCGCATTCTCGGTAACGCGGCAGATTACCTTGCTGATGATGGCGTGTTGATTTGTGAAGTCGGCAACAGCATGGT
ACATCTTATGGAACAATATCCGGATGTTCCGTTCACCTGGCTGGAGTTTGATAACGGCGGCGATGGTGTGTTTATGCTCA
CCAAAGAGCAGCTTATTGCCGCACGAGAACATTTCGCGATTTATAAAGATTAA

Upstream 100 bases:

>100_bases
ACGTATTTTTCCTCACTCACCGCGCGTGAATGCTGATTTATCGTCATCTTCATGGCAAACTAGCCGCCGAAATTATTGCG
ATCATGCCCTGGAGGAATAC

Downstream 100 bases:

>100_bases
GTAAACACGCAAACACAACAATAACGGAGCCGTGATGGCTGGAAACACAATTGGACAACTCTTTCGCGTAACCACCTTCG
GCGAATCGCACGGGCTGGCG

Product: N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDEAVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERV
IRRVNERIPVAYLTNKAWFCGHEFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSDLPNEYRHEPELGLASGTDGLKL
TRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDVPFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD

Sequences:

>Translated_310_residues
MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDEAVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERV
IRRVNERIPVAYLTNKAWFCGHEFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSDLPNEYRHEPELGLASGTDGLKL
TRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDVPFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD
>Mature_310_residues
MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDEAVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERV
IRRVNERIPVAYLTNKAWFCGHEFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSDLPNEYRHEPELGLASGTDGLKL
TRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDVPFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD

Specific function: Unknown

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family

Homologues:

Organism=Homo sapiens, GI7705409, Length=231, Percent_Identity=29.004329004329, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI87082085, Length=310, Percent_Identity=100, Blast_Score=642, Evalue=0.0,
Organism=Escherichia coli, GI1787463, Length=194, Percent_Identity=32.9896907216495, Blast_Score=102, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24582226, Length=199, Percent_Identity=27.6381909547739, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YFCB_ECOLI (P39199)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   M27714
- EMBL:   Y00720
- EMBL:   M33021
- PIR:   H65005
- RefSeq:   AP_002930.1
- RefSeq:   NP_416833.4
- ProteinModelPortal:   P39199
- SMR:   P39199
- DIP:   DIP-11976N
- MINT:   MINT-1267343
- STRING:   P39199
- EnsemblBacteria:   EBESCT00000001502
- EnsemblBacteria:   EBESCT00000015178
- GeneID:   946805
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5841
- KEGG:   eco:b2330
- EchoBASE:   EB2343
- EcoGene:   EG12449
- eggNOG:   COG2890
- GeneTree:   EBGT00050000009834
- HOGENOM:   HBG732041
- OMA:   VGNSQVH
- ProtClustDB:   PRK11805
- BioCyc:   EcoCyc:EG12449-MONOMER
- BioCyc:   MetaCyc:EG12449-MONOMER
- Genevestigator:   P39199
- GO:   GO:0005622
- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR017127
- InterPro:   IPR007848
- PIRSF:   PIRSF037167
- TIGRFAMs:   TIGR00536
- TIGRFAMs:   TIGR03533

Pfam domain/function: PF05175 MTS

EC number: =2.1.1.72

Molecular weight: Translated: 35002; Mature: 35002

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDEAVQLVLPSLYLPLDIP
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCC
EDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGHEFYVDERVLVPRSPIGEL
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECCEEEECCEEEECCEEECCCCHHHHH
INNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVDAVDISPDALAVAEQNIEEHG
HHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEHHCCCCCCEEEECCCCHHHHHHHCHHHCC
LIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSDLPNEYRHEPELGLASGTDGLKL
CCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCCHHHCCCCCCCCCCCCCHHHH
TRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDVPFTWLEFDNGGDGVFMLTKEQLIA
HHHHHCCHHHHHCCCCEEEEECCCHHHHHHHHCCCCCEEEEEECCCCCEEEEEEHHHHHH
AREHFAIYKD
HHHHEEEECC
>Mature Secondary Structure
MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDEAVQLVLPSLYLPLDIP
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCC
EDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGHEFYVDERVLVPRSPIGEL
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECCEEEECCEEEECCEEECCCCHHHHH
INNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVDAVDISPDALAVAEQNIEEHG
HHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEHHCCCCCCEEEECCCCHHHHHHHCHHHCC
LIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSDLPNEYRHEPELGLASGTDGLKL
CCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCCHHHCCCCCCCCCCCCCHHHH
TRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDVPFTWLEFDNGGDGVFMLTKEQLIA
HHHHHCCHHHHHCCCCEEEEECCCHHHHHHHHCCCCCEEEEEECCCCCEEEEEEHHHHHH
AREHFAIYKD
HHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2182772; 2969724; 7984428