Click here to switch to the map view.

The map label for this gene is mnmC [H]

Identifier: 157161812

GI number: 157161812

Start: 2483335

End: 2485341

Strand: Direct

Name: mnmC [H]

Synonym: EcHS_A2475

Alternate gene names: 157161812

Gene position: 2483335-2485341 (Clockwise)

Preceding gene: 157161809

Following gene: 157161820

Centisome position: 53.48

GC content: 52.72

Gene sequence:

>2007_bases
GTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGT
CTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTACGTTTTTCTGGGAGGTAACCAATTAGAGGTACGCTTTCCTG
AGCATCCACATCCTCTGTTTGTGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTTTGGCAGGCATTT
GATCAGTTTCGCGAAGCACATCCGCAAGCGCGATTACAACGCTTACATTTCATTAGTTTTGAGAAATTTCCCCTCACCCG
TGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGC
CCTTGCCCGGTTGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTG
ACCAGCCAACTGGACGATTCGCTAAATCAAAAAGTAGATGCCTGGTTTCTGGACGGCTTTGCGCCAGCTAAAAACCCGGA
TATGTGGACGCAAAATCTGTTTAACGCCATGGCAAGGTTGGCGCGTCCAGGCGGCACGCTGGCGACATTTACGTCTGCCG
GTTTTGTCCGCCGCGGTTTGCAGGAAGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTT
TGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGC
GGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATT
GCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAG
GCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGA
CTGGTGCGGCGTCACGCAGTTAGGCTGGGATGAGAAAAGCCAGCATAAAATCGCACAGATGTTGTCAATGGATTTACCCG
CAGAACTGGCTGTAGCCGTTGAGGCAAATGCGGTTGAACAAATTACGGGCGTTGCGACAAATTGCAGCGGCATTACTTAT
CCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTA
TTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACAC
ACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCC
GGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCC
ACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGACG
ATCAGCAGCAGAATCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAA
GAGGCGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACT
CGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGG
CTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATGAGCGACGAACCGATTCCG
ATGGATGCCAGTACGCTGGCGGCGTTAAACCCGAATCGGTTATGGGTGCGGAAATTGTTGAAGGGTAAAGCGGTTAAGGC
GGGGTAA

Upstream 100 bases:

>100_bases
GTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGC
CTGTAAGACGAGTAACTTAC

Downstream 100 bases:

>100_bases
TCTGCTCTCGCAGTGTTTGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCG
ATAAGCGTCGCATCAGGCAT

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 668; Mature: 668

Protein sequence:

>668_residues
MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAF
DQFREAHPQARLQRLHFISFEKFPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRRGLQEAGFTMQKRKGFGRKREML
CGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIASALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDE
ALNRFFSNAFTFARRFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVATNCSGITY
PQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQQATHSVVVLANGHQISRFSQTSTLPVYSVA
GQVSHIPTTPELAELKQVLCYDGYLTPQNPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDK
EARCGVRCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAPLCAEILAAQMSDEPIP
MDASTLAALNPNRLWVRKLLKGKAVKAG

Sequences:

>Translated_668_residues
MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAF
DQFREAHPQARLQRLHFISFEKFPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRRGLQEAGFTMQKRKGFGRKREML
CGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIASALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDE
ALNRFFSNAFTFARRFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVATNCSGITY
PQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQQATHSVVVLANGHQISRFSQTSTLPVYSVA
GQVSHIPTTPELAELKQVLCYDGYLTPQNPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDK
EARCGVRCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAPLCAEILAAQMSDEPIP
MDASTLAALNPNRLWVRKLLKGKAVKAG
>Mature_668_residues
MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAF
DQFREAHPQARLQRLHFISFEKFPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRRGLQEAGFTMQKRKGFGRKREML
CGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIASALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDE
ALNRFFSNAFTFARRFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVATNCSGITY
PQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQQATHSVVVLANGHQISRFSQTSTLPVYSVA
GQVSHIPTTPELAELKQVLCYDGYLTPQNPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDK
EARCGVRCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAPLCAEILAAQMSDEPIP
MDASTLAALNPNRLWVRKLLKGKAVKAG

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=668, Percent_Identity=99.7005988023952, Blast_Score=1386, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 74477; Mature: 74477

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNQLEVRFPEHPHPLF
CCCCCCCCCCEEECCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCEEEEECCCCCCCEE
VVAESGFGTGLNFLTLWQAFDQFREAHPQARLQRLHFISFEKFPLTRADLALAHQHWPEL
EEEECCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHEECCCCCCHHHHHHHHHCCCCC
APWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINELTSQLDDSLNQKVDAWFLDGF
CHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEECCC
APAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRRGLQEAGFTMQKRKGFGRKREML
CCCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHH
CGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIASALLSLALLRRGWQVTLYCADEAPA
HHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCCC
LGASGNRQGALYPLLSKHDEALNRFFSNAFTFARRFYDQLPVKFDHDWCGVTQLGWDEKS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHH
QHKIAQMLSMDLPAELAVAVEANAVEQITGVATNCSGITYPQGGWLCPAELTRNVLELAQ
HHHHHHHHHCCCCCCEEEEEECHHHHHHHCCEECCCCCCCCCCCEECHHHHHHHHHHHHH
QQGLQIYYQYQLQNLSRKDDCWLLNFAGDQQATHSVVVLANGHQISRFSQTSTLPVYSVA
HCCCEEEEEEEHHCCCCCCCEEEEEECCCCCCCCEEEEEECCCEECHHCCCCCCCHHHHC
GQVSHIPTTPELAELKQVLCYDGYLTPQNPANQHHCIGASYHRGSEDTAYSEDDQQQNRQ
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHH
RLIDCFPQAQWAKEVDVSDKEARCGVRCATRDHLPMVGNVPDYEATLVEYASLAEQKDEA
HHHHHCCCHHHHHHCCCCCCHHCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VSAPVFDDLFMFAALGSRGLCSAPLCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLL
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHEECCCCHHHHHHHH
KGKAVKAG
CCCCCCCC
>Mature Secondary Structure
MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNQLEVRFPEHPHPLF
CCCCCCCCCCEEECCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCEEEEECCCCCCCEE
VVAESGFGTGLNFLTLWQAFDQFREAHPQARLQRLHFISFEKFPLTRADLALAHQHWPEL
EEEECCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHEECCCCCCHHHHHHHHHCCCCC
APWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINELTSQLDDSLNQKVDAWFLDGF
CHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEECCC
APAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRRGLQEAGFTMQKRKGFGRKREML
CCCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHH
CGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIASALLSLALLRRGWQVTLYCADEAPA
HHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCCC
LGASGNRQGALYPLLSKHDEALNRFFSNAFTFARRFYDQLPVKFDHDWCGVTQLGWDEKS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHH
QHKIAQMLSMDLPAELAVAVEANAVEQITGVATNCSGITYPQGGWLCPAELTRNVLELAQ
HHHHHHHHHCCCCCCEEEEEECHHHHHHHCCEECCCCCCCCCCCEECHHHHHHHHHHHHH
QQGLQIYYQYQLQNLSRKDDCWLLNFAGDQQATHSVVVLANGHQISRFSQTSTLPVYSVA
HCCCEEEEEEEHHCCCCCCCEEEEEECCCCCCCCEEEEEECCCEECHHCCCCCCCHHHHC
GQVSHIPTTPELAELKQVLCYDGYLTPQNPANQHHCIGASYHRGSEDTAYSEDDQQQNRQ
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHH
RLIDCFPQAQWAKEVDVSDKEARCGVRCATRDHLPMVGNVPDYEATLVEYASLAEQKDEA
HHHHHCCCHHHHHHCCCCCCHHCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VSAPVFDDLFMFAALGSRGLCSAPLCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLL
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHEECCCCHHHHHHHH
KGKAVKAG
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA