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The map label for this gene is nuoE

Identifier: 157161773

GI number: 157161773

Start: 2443123

End: 2443623

Strand: Reverse

Name: nuoE

Synonym: EcHS_A2434

Alternate gene names: 157161773

Gene position: 2443623-2443123 (Counterclockwise)

Preceding gene: 157161774

Following gene: 157161772

Centisome position: 52.62

GC content: 55.29

Gene sequence:

>501_bases
ATGCACGAGAATCAACAACCACAAACCGAGGCTTTTGAGCTGAGTGCGGCAGAGCGTGAAGCGATTGAGCACGAGATGCA
CCACTACGAAGACCCGCGTGCGGCGTCCATTGAAGCGCTGAAAATCGTTCAGAAGCAGCGTGGCTGGGTGCCGGATGGTG
CGATCCACGCGATCGCCGATGTGCTGGGTATTCCGGCAAGCGACGTCGAAGGTGTGGCAACGTTCTACAGTCAGATCTTC
CGTCAGCCGGTTGGTCGCCATGTGATCCGTTATTGCGACAGCGTGGTCTGTCATATCAACGGGTATCAGGGTATTCAGGC
GGCGCTCGAGAAAAAGCTGAACATCAAACCAGGGCAAACGACATTTGATGGCCGCTTTACGCTGCTGCCAACTTGCTGCC
TGGGGAACTGTGATAAAGGGCCAAACATGATGATCGATGAGGACACTCACGCGCATCTGACCCCGGAAGCGATCCCTGAA
CTGCTGGAGCGGTATAAATGA

Upstream 100 bases:

>100_bases
TGCAGCAAATTCCGGCGGCGATCCGCGGCAGCCTGGTGTCTGACCTGATTGTTTATCTGGGCAGTATCGATTTTGTTATG
TCAGATGTGGACCGCTAATT

Downstream 100 bases:

>100_bases
AAAACATTATCCGTACTCCCGAAACGCATCCGCTGACCTGGCGTCTGCGCGATGACAAACAGCCAGTGTGGCTGGACGAA
TACCGCAGCAAAAACGGTTA

Product: NADH dehydrogenase subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E; NUO5

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQKQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIF
RQPVGRHVIRYCDSVVCHINGYQGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
LLERYK

Sequences:

>Translated_166_residues
MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQKQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIF
RQPVGRHVIRYCDSVVCHINGYQGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
LLERYK
>Mature_166_residues
MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQKQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIF
RQPVGRHVIRYCDSVVCHINGYQGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
LLERYK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family

Homologues:

Organism=Homo sapiens, GI222080062, Length=157, Percent_Identity=35.031847133758, Blast_Score=103, Evalue=5e-23,
Organism=Escherichia coli, GI1788621, Length=166, Percent_Identity=100, Blast_Score=349, Evalue=7e-98,
Organism=Caenorhabditis elegans, GI17561328, Length=157, Percent_Identity=36.9426751592357, Blast_Score=112, Evalue=8e-26,
Organism=Drosophila melanogaster, GI18859877, Length=157, Percent_Identity=33.7579617834395, Blast_Score=102, Evalue=8e-23,
Organism=Drosophila melanogaster, GI21355353, Length=153, Percent_Identity=30.0653594771242, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUOE_ECOL6 (P0AFD2)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_754712.1
- ProteinModelPortal:   P0AFD2
- SMR:   P0AFD2
- EnsemblBacteria:   EBESCT00000040439
- GeneID:   1038365
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c2826
- GeneTree:   EBGT00050000011839
- HOGENOM:   HBG616790
- OMA:   IVQKERG
- ProtClustDB:   PRK07539
- BRENDA:   1.6.99.5
- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335
- Gene3D:   G3DSA:3.40.30.10
- PANTHER:   PTHR10371
- PIRSF:   PIRSF000216
- TIGRFAMs:   TIGR01958

Pfam domain/function: PF01257 Complex1_24kDa; SSF52833 Thiordxn-like_fd

EC number: =1.6.99.5

Molecular weight: Translated: 18590; Mature: 18590

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQKQRGWVPDGAIHAIAD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
VLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGYQGIQAALEKKLNIKPGQT
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCCCCCC
TFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPELLERYK
CCCCCEEEEHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQKQRGWVPDGAIHAIAD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
VLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGYQGIQAALEKKLNIKPGQT
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCCCCCC
TFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPELLERYK
CCCCCEEEEHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157