Click here to switch to the map view.

The map label for this gene is nuoF [H]

Identifier: 157161772

GI number: 157161772

Start: 2441789

End: 2443126

Strand: Reverse

Name: nuoF [H]

Synonym: EcHS_A2433

Alternate gene names: 157161772

Gene position: 2443126-2441789 (Counterclockwise)

Preceding gene: 157161773

Following gene: 157161771

Centisome position: 52.61

GC content: 56.43

Gene sequence:

>1338_bases
ATGAAAAACATTATCCGTACTCCCGAAACGCATCCGCTGACCTGGCGTCTGCGCGATGACAAACAGCCAGTGTGGCTGGA
CGAATACCGCAGCAAAAACGGTTACGAAGGGGCGCGTAAGGCGCTGACCGGGCTGTCTCCGGACGAAATCGTTAATCAGG
TAAAAGACGCTGGTCTGAAAGGGCGCGGCGGCGCGGGCTTTTCGACTGGCCTGAAGTGGAGCCTGATGCCGAAAGACGAA
TCCATGAACATCCGTTACCTGCTGTGTAACGCCGATGAAATGGAGCCGGGCACCTATAAAGACCGCCTGTTGATGGAGCA
ACTGCCGCACCTGCTGGTGGAAGGTATGCTCATCTCCGCGTTTGCGCTGAAAGCTTACCGTGGCTACATCTTCCTGCGTG
GCGAATATATCGAAGCGGCAGTAAATCTGCGCCGCGCCATTGCCGAAGCCACTGAAGCGGGCTTGCTTGGCAAAAACATT
ATGGGAACAGGTTTCGACTTCGAACTGTTCGTCCATACCGGGGCAGGGCGCTATATCTGCGGGGAAGAAACAGCGTTAAT
CAACTCCCTGGAAGGGCGTCGTGCTAACCCACGCTCGAAACCCCCCTTCCCGGCAACCTCCGGCGTATGGGGCAAACCGA
CCTGTGTCAACAACGTCGAAACCCTGTGTAACGTTCCGGCGATCCTCGCTAACGGCGTGGAGTGGTATCAGAACATCTCG
AAAAGTAAAGATGCTGGCACCAAGCTGATGGGCTTCTCCGGTCGGGTGAAAAATCCGGGACTGTGGGAACTGCCGTTTGG
TACTACCGCGCGCGAGATCCTCGAAGATTACGCCGGTGGTATGCGTGACGGTCTGAAATTCAAAGCCTGGCAGCCAGGCG
GCGCGGGCACCGACTTCCTGACCGAAGCGCACCTTGACCTGCCGATGGAATTCGAAAGTATCGGTAAAGCGGGAAGCCGT
CTGGGTACGGCGCTGGCGATGGCGGTTGACCATGAGATCAACATGGTGTCGCTGGTGCGTAACCTGGAAGAGTTTTTCGC
CCGTGAGTCCTGCGGCTGGTGTACGCCGTGCCGCGACGGTCTGCCGTGGAGCGTGAAAATTCTGCGTGCGCTGGAGCGTG
GTGAAGGTCAGCCGGGCGATATCGAAACACTTGAGCAACTGTGTCGATTCTTAGGCCCGGGTAAAACTTTCTGTGCCCAC
GCACCTGGTGCAGTGGAGCCGTTACAGAGCGCCATCAAATATTTCCGCGAAGAATTTGAGGCGGGAATCAAACAGCCGTT
CAGCAATACCCATTTGATTAATGGGATTCAGCCGAACCTGCTGAAAGAGCGCTGGTAA

Upstream 100 bases:

>100_bases
GCCTGGGGAACTGTGATAAAGGGCCAAACATGATGATCGATGAGGACACTCACGCGCATCTGACCCCGGAAGCGATCCCT
GAACTGCTGGAGCGGTATAA

Downstream 100 bases:

>100_bases
CCGAATTTCGATTAACGCTCAGTCTCTGACTGAGAAAACTGGAAGCATGCTAATGGCTACAATTCATGTAGACGGCAAAG
AATACGAGGTCAACGGAGCG

Product: NADH dehydrogenase I subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F; NDH-1 subunit F; NUO6 [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTGLSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDE
SMNIRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNNVETLCNVPAILANGVEWYQNIS
KSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILEDYAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSR
LGTALAMAVDHEINMVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLGPGKTFCAH
APGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW

Sequences:

>Translated_445_residues
MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTGLSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDE
SMNIRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNNVETLCNVPAILANGVEWYQNIS
KSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILEDYAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSR
LGTALAMAVDHEINMVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLGPGKTFCAH
APGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW
>Mature_445_residues
MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTGLSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDE
SMNIRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNNVETLCNVPAILANGVEWYQNIS
KSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILEDYAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSR
LGTALAMAVDHEINMVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLGPGKTFCAH
APGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=376, Percent_Identity=44.4148936170213, Blast_Score=324, Evalue=9e-89,
Organism=Homo sapiens, GI20149568, Length=376, Percent_Identity=44.4148936170213, Blast_Score=324, Evalue=1e-88,
Organism=Escherichia coli, GI1788620, Length=445, Percent_Identity=99.7752808988764, Blast_Score=915, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17531727, Length=375, Percent_Identity=42.4, Blast_Score=305, Evalue=3e-83,
Organism=Drosophila melanogaster, GI19920794, Length=375, Percent_Identity=44.8, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI45550452, Length=378, Percent_Identity=44.1798941798942, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI24653819, Length=377, Percent_Identity=34.7480106100796, Blast_Score=241, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24653817, Length=352, Percent_Identity=32.6704545454545, Blast_Score=209, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 49321; Mature: 49321

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTGLSPDEIVNQVKDAGLK
CCCCCCCCCCCCEEEEECCCCCCCCHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCC
GRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLISA
CCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNIMGTGFDFELFVHTGAGRYIC
HHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEE
GEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNNVETLCNVPAILANGVEWYQNIS
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHCCHHHHHHHH
KSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILEDYAGGMRDGLKFKAWQPGGAGTDFL
CCCCCCCEEECCCCCCCCCCEEECCCCCHHHHHHHHHCCCHHCCCEEEEECCCCCCCHHH
TEAHLDLPMEFESIGKAGSRLGTALAMAVDHEINMVSLVRNLEEFFARESCGWCTPCRDG
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
LPWSVKILRALERGEGQPGDIETLEQLCRFLGPGKTFCAHAPGAVEPLQSAIKYFREEFE
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHH
AGIKQPFSNTHLINGIQPNLLKERW
HHHHCCCCCCCEECCCCCHHHHCCC
>Mature Secondary Structure
MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTGLSPDEIVNQVKDAGLK
CCCCCCCCCCCCEEEEECCCCCCCCHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCC
GRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLISA
CCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNIMGTGFDFELFVHTGAGRYIC
HHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEE
GEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNNVETLCNVPAILANGVEWYQNIS
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHCCHHHHHHHH
KSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILEDYAGGMRDGLKFKAWQPGGAGTDFL
CCCCCCCEEECCCCCCCCCCEEECCCCCHHHHHHHHHCCCHHCCCEEEEECCCCCCCHHH
TEAHLDLPMEFESIGKAGSRLGTALAMAVDHEINMVSLVRNLEEFFARESCGWCTPCRDG
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
LPWSVKILRALERGEGQPGDIETLEQLCRFLGPGKTFCAHAPGAVEPLQSAIKYFREEFE
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHH
AGIKQPFSNTHLINGIQPNLLKERW
HHHHCCCCCCCEECCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7690854; 8157582; 9205837; 9278503; 8366049 [H]