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The map label for this gene is rhmR

Identifier: 157161733

GI number: 157161733

Start: 2402216

End: 2402998

Strand: Reverse

Name: rhmR

Synonym: EcHS_A2390

Alternate gene names: 157161733

Gene position: 2402998-2402216 (Counterclockwise)

Preceding gene: 157161734

Following gene: 157161732

Centisome position: 51.75

GC content: 50.96

Gene sequence:

>783_bases
ATGTTGGAATCAAGCAAAGTTCCCGCCCTGACACGGGCTATTGATATTCTCAATTTGATTGCCCGCATTGGCCCCTGTAG
TGCTGCGACGATCATTGACACATTGGGAATCCCTAAAAGCACGGCCTATTTGCTGCTTAATGAACTCAGGCGTCAGCGTT
TTCTTAGCCTTGATCACCAGGAAAATTTTTGTTTGTGGACCAGGCTGGTGGAGCTTTCCGGTCATGCGTTAAGCAAGATG
GATCTCCGGGAACTGGCTCGACCGCGTTTGACGCAACTGATGGATACCACCGGGCTGTTATGTCACCTGGGGATTATTGA
TAACGGCAGCGCTTATTACATTTTGAAAGTGGAGTCATCAGCCACTATCAGCGTGCGTTCCCATGAAGGAAAAAGCCTTT
CGCTTTATCGCTCCGGAATAGGTAAATGCCTGCTTGCCTGGCAACCTGCAGCGGTACAGCAAAGTATTATCGAAGGGCTG
GTCTGGGAGCAGGCGACACCCACAACGATTACTCATCCACAGCAACTTCACGAAGAACTGGCGCGTATTCGCCGTCAGGG
CTGGAGCTATGACAACGGCGAAGATTACGCCGATGTCCGTTGTGTCGCTGCGCCGGTGTTTAACGCCAATAACGAGCTTA
CCGCTGCGATCTCGGTGGTCGGTACCCGCTTACAAATCAATGAAGAATACCGTGATTATCTGGCGGGCAAGGCCATTGCC
TGCGCCAGGGATATTTCACGTCTGTTGGGATGGAAAAGTCCCTTTGACTTACAAGCCTCATAA

Upstream 100 bases:

>100_bases
TCTCCAGTCTGCTGGACGAGGAGCGGGATTTGATGCGCTTCACAATTTTACTCCCCCTGAGCAACGAGACTGATTCCAGT
ATCTAAGACTGGAGTCCAGT

Downstream 100 bases:

>100_bases
CAATGGAGAACATCATGACCCTACCAAAAATTAAACAGGTTCGCGCCTGGTTTACTGGCGGTGCGACAGCAGAAAAAGGC
GCTGGCGGCGGTGATTATCA

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYLLLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKM
DLRELARPRLTQLMDTTGLLCHLGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAISVVGTRLQINEEYRDYLAGKAIA
CARDISRLLGWKSPFDLQAS

Sequences:

>Translated_260_residues
MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYLLLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKM
DLRELARPRLTQLMDTTGLLCHLGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAISVVGTRLQINEEYRDYLAGKAIA
CARDISRLLGWKSPFDLQAS
>Mature_260_residues
MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYLLLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKM
DLRELARPRLTQLMDTTGLLCHLGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAISVVGTRLQINEEYRDYLAGKAIA
CARDISRLLGWKSPFDLQAS

Specific function: Unknown

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain

Homologues:

Organism=Escherichia coli, GI1788581, Length=260, Percent_Identity=100, Blast_Score=533, Evalue=1e-153,
Organism=Escherichia coli, GI1790752, Length=246, Percent_Identity=31.7073170731707, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1786468, Length=251, Percent_Identity=30.6772908366534, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI1788131, Length=222, Percent_Identity=32.8828828828829, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI87082359, Length=251, Percent_Identity=24.7011952191235, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1786715, Length=248, Percent_Identity=28.2258064516129, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1789999, Length=238, Percent_Identity=26.890756302521, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): RHMR_ECOLI (P77732)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   F64995
- RefSeq:   AP_002845.1
- RefSeq:   NP_416751.1
- ProteinModelPortal:   P77732
- SMR:   P77732
- DIP:   DIP-11956N
- IntAct:   P77732
- STRING:   P77732
- EnsemblBacteria:   EBESCT00000003331
- EnsemblBacteria:   EBESCT00000018260
- GeneID:   944752
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2242
- KEGG:   eco:b2248
- EchoBASE:   EB3839
- EcoGene:   EG14086
- eggNOG:   COG1414
- GeneTree:   EBGT00050000008790
- HOGENOM:   HBG739407
- OMA:   IESPATI
- ProtClustDB:   CLSK880340
- BioCyc:   EcoCyc:G7161-MONOMER
- Genevestigator:   P77732
- GO:   GO:0006350
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- SMART:   SM00346

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR

EC number: NA

Molecular weight: Translated: 28905; Mature: 28905

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: PS51077 HTH_ICLR; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYLLLNELRRQRFLSLDHQ
CCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCC
ENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCHLGIIDNGSAYYILKVESS
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEEEECCC
ATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGLVWEQATPTTITHPQQLHEEL
CEEEEECCCCCCHHHHHHCHHHEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
ARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAISVVGTRLQINEEYRDYLAGKAIA
HHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHEEECHHHHHHHHCCHHH
CARDISRLLGWKSPFDLQAS
HHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYLLLNELRRQRFLSLDHQ
CCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCC
ENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCHLGIIDNGSAYYILKVESS
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEEEECCC
ATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGLVWEQATPTTITHPQQLHEEL
CEEEEECCCCCCHHHHHHCHHHEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
ARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAISVVGTRLQINEEYRDYLAGKAIA
HHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHEEECHHHHHHHHCCHHH
CARDISRLLGWKSPFDLQAS
HHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503