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The map label for this gene is rhmD [H]

Identifier: 157161732

GI number: 157161732

Start: 2400996

End: 2402213

Strand: Reverse

Name: rhmD [H]

Synonym: EcHS_A2389

Alternate gene names: 157161732

Gene position: 2402213-2400996 (Counterclockwise)

Preceding gene: 157161733

Following gene: 157161731

Centisome position: 51.73

GC content: 52.22

Gene sequence:

>1218_bases
ATGGAGAACATCATGACCCTACCAAAAATTAAACAGGTTCGCGCCTGGTTTACTGGCGGTGCGACAGCAGAAAAAGGCGC
TGGCGGCGGTGATTATCACGACCAGGGGGCGAATCACTGGATAGACGATCATATTGCCACCCCGATGAGTAAATACCGCG
ATTACGAGCAGTCACGCCAGTCATTTGGCATTAACGTTCTTGGCACGTTGGTTGTTGAAGTCGAAGCAGAAAACGGCCAG
ACCGGATTCGCCGTTTCGACAGCCGGTGAAATGGGCTGTTTTATTGTCGAAAAACATCTTAACCGTTTCATTGAGGGTAA
ATGTGTCAGTGATATCAAACTGATCCACGATCAAATGCTCAGTGCCACCCTGTATTACTCCGGCTCTGGTGGCCTGGTGA
TGAATACGATTTCCTGTGTCGATCTGGCTCTGTGGGATCTGTTCGGCAAAGTGGTCGGGCTTCCGGTTTATAAACTTTTA
GGCGGCGCTGTTCGTGATGAGATTCAGTTCTACGCCACAGGTGCGCGTCCGGATCTGGCAAAAGAGATGGGCTTTATCGG
TGGCAAAATGCCGACGCACTGGGGGCCACATGATGGCGATGCGGGGATCCGCAAAGATGCCGCTATGGTCGCGGATATGC
GTGAAAAATGCGGTGAGGATTTCTGGTTAATGCTCGACTGCTGGATGAGTCAGGACGTGAACTATGCGACCAAACTGGCC
CACGCTTGCGCGCCCTATAACCTGAAATGGATCGAAGAGTGCCTGCCGCCACAGCAGTATGAAGGTTATCGCGAACTGAA
ACGCAACGCGCCAGTCGGAATGATGGTCACCAGCGGTGAGCACCACGGCACACTGCAATCTTTTCGTACGCTTTCAGAAA
CCGGTATCGACATTATGCAGCCGGATGTTGGCTGGTGCGGTGGTTTAACCACGCTGGTGGAAATTGCCGCAATCGCCAAA
TCCAGGGGGCAACTGGTGGTGCCGCACGGTTCGTCTGTTTACTCTCACCATGCGGTGATCACCTTCACCAATACGCCATT
CAGCGAATTCCTGATGACCAGCCCGGATTGTTCAACGATGCGTCCGCAGTTTGACCCGATTCTGCTCAATGAGCCGGTTC
CGGTGAATGGTCGTATTCATAAATCAGTGCTTGATAAACCCGGTTTTGGCGTCGAACTCAATCGTGACTGCAATTTGAAA
CGCCCCTACAGCCACTAA

Upstream 100 bases:

>100_bases
AATACCGTGATTATCTGGCGGGCAAGGCCATTGCCTGCGCCAGGGATATTTCACGTCTGTTGGGATGGAAAAGTCCCTTT
GACTTACAAGCCTCATAACA

Downstream 100 bases:

>100_bases
TCACTCCGGGCGTTGCTGTGACAACGGTAACGCCCACATATTCTAAGGAGCTTTTTATGAGCACCGCTTTGCTTGACGCC
GTGGTGAAGAAAAACCGTGT

Product: mandelate racemase/muconate lactonizing enzyme family protein

Products: NA

Alternate protein names: RhamD [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MENIMTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATPMSKYRDYEQSRQSFGINVLGTLVVEVEAENGQ
TGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQMLSATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLL
GGAVRDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFWLMLDCWMSQDVNYATKLA
HACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGEHHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAK
SRGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRDCNLK
RPYSH

Sequences:

>Translated_405_residues
MENIMTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATPMSKYRDYEQSRQSFGINVLGTLVVEVEAENGQ
TGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQMLSATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLL
GGAVRDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFWLMLDCWMSQDVNYATKLA
HACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGEHHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAK
SRGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRDCNLK
RPYSH
>Mature_405_residues
MENIMTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATPMSKYRDYEQSRQSFGINVLGTLVVEVEAENGQ
TGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQMLSATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLL
GGAVRDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFWLMLDCWMSQDVNYATKLA
HACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGEHHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAK
SRGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRDCNLK
RPYSH

Specific function: Catalyzes the dehydration of L-rhamnonate to 2-keto-3- deoxy-L-rhamnonate (KDR) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily [H]

Homologues:

Organism=Escherichia coli, GI226510960, Length=401, Percent_Identity=99.7506234413965, Blast_Score=840, Evalue=0.0,
Organism=Escherichia coli, GI48994953, Length=243, Percent_Identity=30.8641975308642, Blast_Score=91, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.90 [H]

Molecular weight: Translated: 44685; Mature: 44685

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENIMTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATPMSKYRDYEQSRQ
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHH
SFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQML
HCCCEEEEEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
SATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAVRDEIQFYATGARPDLA
HEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHHCEEEEECCCCCHHH
KEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFWLMLDCWMSQDVNYATKLA
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHCCCCCHHHHHH
HACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGEHHGTLQSFRTLSETGIDIMQ
HHHCCCCHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEC
PDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTM
CCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCEECCEEEEEECCCCHHHHHCCCCCCCCC
RPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRDCNLKRPYSH
CCCCCCEEECCCCCCCCCHHHHHHCCCCCCEEECCCCCCCCCCCC
>Mature Secondary Structure
MENIMTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATPMSKYRDYEQSRQ
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHH
SFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQML
HCCCEEEEEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
SATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAVRDEIQFYATGARPDLA
HEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHHCEEEEECCCCCHHH
KEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFWLMLDCWMSQDVNYATKLA
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHCCCCCHHHHHH
HACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGEHHGTLQSFRTLSETGIDIMQ
HHHCCCCHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEC
PDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTM
CCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCEECCEEEEEECCCCHHHHHCCCCCCCCC
RPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRDCNLKRPYSH
CCCCCCEEECCCCCCCCCHHHHHHCCCCCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA