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The map label for this gene is glpA

Identifier: 157161725

GI number: 157161725

Start: 2393629

End: 2395257

Strand: Direct

Name: glpA

Synonym: EcHS_A2382

Alternate gene names: 157161725

Gene position: 2393629-2395257 (Clockwise)

Preceding gene: 157161724

Following gene: 157161726

Centisome position: 51.55

GC content: 57.27

Gene sequence:

>1629_bases
ATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTG
TGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGC
TGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGC
ATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTT
TATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTA
ACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTGGAT
GCCAAAGAACACGGTGCCGTTATCCTTACCGCTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGTGT
TCGTGTACGTAACCATCTCACCGGCGAAACTCAGGCCCTTCATGCACCTGTCGTGGTTAATGCCGCTGGGATCTGGGGGC
AACACATTGCCGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGATCGCTGCTGATCATGGATCACCGCATT
AACCAGCATGTGATCAACCGCTGCCGTAAACCTTCCGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTGATTGG
TACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAGGTTGATATTCTGCTGCGTG
AAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGGGCCTATTCTGGCGTGCGCCCGCTGGTTGCCAGC
GATGACGACCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGATT
TATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGG
GCAACACGCGCCCCTGTACGACTGCCGATCTGGCACTGCCTGGTTCACAAGAACCCGCTGAAGTTACCTTGCGTAAAGTC
ATCTCCCTGCCTGCCCCGCTGCGCGGTTCTGCGGTTTATCGTCATGGCGATCGCACGCCTGCCTGGCTGAGCGAAGGCCG
TCTGCACCGTAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCGGTAGAAAATTTAAACGTTA
ATAGCCTGCTGGATTTACGCCGTCGTACCCGTGTGGGGATGGGCACCTGCCAGGGCGAACTCTGCGCCTGCCGCGCTGCC
GGACTGCTGCAACGTTTTAACGTCACGACGTCTGCGCAATCTATCGAGCAACTTTCCACCTTCCTTAACGAACGCTGGAA
AGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTC
TGGAGAAGGAGCAGAAAGATGCGCTTTGA

Upstream 100 bases:

>100_bases
CACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAA
CGAACTTCAGAGGGATAACA

Downstream 100 bases:

>100_bases
TACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTG
TCACGCGTGGTCAAAGCGCG

Product: sn-glycerol-3-phosphate dehydrogenase subunit A

Products: NA

Alternate protein names: G-3-P dehydrogenase

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIATGATGRNHGLLHSGARYAVTDAESARECISENQILKR
IARHCVEPTNGLFITLPEDDLSFQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAEYADLRIRMFPAKGSLLIMDHRI
NQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYNEIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVAS
DDDPSGRNVSRGIVLLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEPAEVTLRKV
ISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVENLNVNSLLDLRRRTRVGMGTCQGELCACRAA
GLLQRFNVTTSAQSIEQLSTFLNERWKGVQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL

Sequences:

>Translated_542_residues
MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIATGATGRNHGLLHSGARYAVTDAESARECISENQILKR
IARHCVEPTNGLFITLPEDDLSFQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAEYADLRIRMFPAKGSLLIMDHRI
NQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYNEIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVAS
DDDPSGRNVSRGIVLLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEPAEVTLRKV
ISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVENLNVNSLLDLRRRTRVGMGTCQGELCACRAA
GLLQRFNVTTSAQSIEQLSTFLNERWKGVQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL
>Mature_542_residues
MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIATGATGRNHGLLHSGARYAVTDAESARECISENQILKR
IARHCVEPTNGLFITLPEDDLSFQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAEYADLRIRMFPAKGSLLIMDHRI
NQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYNEIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVAS
DDDPSGRNVSRGIVLLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEPAEVTLRKV
ISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVENLNVNSLLDLRRRTRVGMGTCQGELCACRAA
GLLQRFNVTTSAQSIEQLSTFLNERWKGVQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL

Specific function: Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses fumarate or nitrate as electron acceptor

COG id: COG0578

COG function: function code C; Glycerol-3-phosphate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=Loosely bound to the cytoplasmic membrane often occurring in vesicles associated with fumarate reductase (By similarity)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI285002233, Length=544, Percent_Identity=25.5514705882353, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI285002231, Length=544, Percent_Identity=25.5514705882353, Blast_Score=116, Evalue=6e-26,
Organism=Escherichia coli, GI1788574, Length=542, Percent_Identity=100, Blast_Score=1104, Evalue=0.0,
Organism=Escherichia coli, GI2367226, Length=380, Percent_Identity=25.5263157894737, Blast_Score=79, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI115534726, Length=417, Percent_Identity=26.8585131894484, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17509253, Length=414, Percent_Identity=29.4685990338164, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI161077125, Length=414, Percent_Identity=28.9855072463768, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24653942, Length=414, Percent_Identity=28.9855072463768, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24653944, Length=414, Percent_Identity=28.9855072463768, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI20130025, Length=414, Percent_Identity=28.9855072463768, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24586295, Length=394, Percent_Identity=25.1269035532995, Blast_Score=80, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLPA_ECO57 (P0A9C1)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   F91019
- RefSeq:   NP_288817.1
- RefSeq:   NP_311153.1
- ProteinModelPortal:   P0A9C1
- SMR:   P0A9C1
- EnsemblBacteria:   EBESCT00000025390
- EnsemblBacteria:   EBESCT00000057701
- GeneID:   916834
- GeneID:   959442
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3499
- KEGG:   ecs:ECs3126
- GeneTree:   EBGT00050000009190
- HOGENOM:   HBG720894
- OMA:   KTFLESC
- ProtClustDB:   PRK11101
- BioCyc:   ECOL83334:ECS3126-MONOMER
- InterPro:   IPR007419
- InterPro:   IPR006076
- InterPro:   IPR017752
- InterPro:   IPR000447
- PRINTS:   PR01001
- TIGRFAMs:   TIGR03377

Pfam domain/function: PF01266 DAO; PF04324 Fer2_BFD

EC number: =1.1.5.3

Molecular weight: Translated: 58959; Mature: 58959

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00977 FAD_G3PDH_1; PS00978 FAD_G3PDH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIATGATGRNHGLLHSGARY
CCCCCCCCCCEEEEECCCCCCCHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCEECCCEE
AVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLSFQATFIRACEEAGISAEA
EEECHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCC
IDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLDAKEHGAVILTAHEVTGLIRE
CCCHHHEEECCCCCCEEEEEEECCCCCCCCEEEHHHHHHCHHHCCEEEEEHHHHHHHHHC
GATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAEYADLRIRMFPAKGSLLIMDHRI
CCCEEEHHHHHCCCCCCCEEECCEEEEECCHHHHHHHHHHCEEEEEEECCCCEEEEECCC
NQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYNEIDDNRVTAEEVDILLREGEKLAP
CHHHHHHCCCCCCCEEEECCCEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHCCCHHHH
VMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIVLLDHAERDGLDGFITITGGKLMTYRL
HHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCEEEHHH
MAEWATDAVCRKLGNTRPCTTADLALPGSQEPAEVTLRKVISLPAPLRGSAVYRHGDRTP
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCC
AWLSEGRLHRSLVCECEAVTAGEVQYAVENLNVNSLLDLRRRTRVGMGTCQGELCACRAA
HHHHCCCCCHHHEEEECCCCCCHHEEHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
GLLQRFNVTTSAQSIEQLSTFLNERWKGVQPIAWGDALRESEFTRWVYQGLCGLEKEQKD
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHC
AL
CC
>Mature Secondary Structure
MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIATGATGRNHGLLHSGARY
CCCCCCCCCCEEEEECCCCCCCHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCEECCCEE
AVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLSFQATFIRACEEAGISAEA
EEECHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCC
IDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLDAKEHGAVILTAHEVTGLIRE
CCCHHHEEECCCCCCEEEEEEECCCCCCCCEEEHHHHHHCHHHCCEEEEEHHHHHHHHHC
GATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAEYADLRIRMFPAKGSLLIMDHRI
CCCEEEHHHHHCCCCCCCEEECCEEEEECCHHHHHHHHHHCEEEEEEECCCCEEEEECCC
NQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYNEIDDNRVTAEEVDILLREGEKLAP
CHHHHHHCCCCCCCEEEECCCEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHCCCHHHH
VMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIVLLDHAERDGLDGFITITGGKLMTYRL
HHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCEEEHHH
MAEWATDAVCRKLGNTRPCTTADLALPGSQEPAEVTLRKVISLPAPLRGSAVYRHGDRTP
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCC
AWLSEGRLHRSLVCECEAVTAGEVQYAVENLNVNSLLDLRRRTRVGMGTCQGELCACRAA
HHHHCCCCCHHHEEEECCCCCCHHEEHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
GLLQRFNVTTSAQSIEQLSTFLNERWKGVQPIAWGDALRESEFTRWVYQGLCGLEKEQKD
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHC
AL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796